A survey of vitreous cell components performed using liquid‐based cytology
Purpose To confirm the efficacy of liquid‐based cytology (LBC) method in the observation of vitreous cells in various vitreoretinal diseases in human. Methods Vitreous fluid samples from 30 eyes were obtained by 23‐gauge 3‐port pars plana vitrectomy. After making three ports, we collected vitreous s...
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| Veröffentlicht in: | Acta ophthalmologica (Oxford, England) England), 2015-08, Vol.93 (5), p.e386-e390 |
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| container_title | Acta ophthalmologica (Oxford, England) |
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| creator | Narumi, Mari Nishitsuka, Koichi Yamakawa, Mitsunori Yamashita, Hidetoshi |
| description | Purpose
To confirm the efficacy of liquid‐based cytology (LBC) method in the observation of vitreous cells in various vitreoretinal diseases in human.
Methods
Vitreous fluid samples from 30 eyes were obtained by 23‐gauge 3‐port pars plana vitrectomy. After making three ports, we collected vitreous specimen from the core vitreous cavity without infusion. We divided the samples into a quiescent group and an active group based on clinical signs of inflammation. To confirm availability of LBC preparation slides for immunostaining, we also performed immunocytochemistry (ICC) for CD68, RPE65 and DEC‐205 (CD205) using LBC slides of 10 cell‐rich cases including retinal detachment and endophthalmitis.
Results
Using LBC method, small amounts of vitreous cells were observed efficiently. Vitreous cells were observed in inflammatory quiescent cases including macular pucker and macular hole. The number of vitreous cells increased significantly in the cases with clinically active inflammation (2297 versus 207 cells/ml, respectively, p |
| doi_str_mv | 10.1111/aos.12623 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1698967260</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3750909731</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5243-2c2048f6baf87ed2687602c9a380c8324b0199508783610ac971d169fd40b6a53</originalsourceid><addsrcrecordid>eNp10M9KwzAABvAgipvTgy8gAS962Jakzb_jGP6DwQ4qeCtpmo6OrumSdtKbj-Az-iRmbu4gmEtC-PHx8QFwidEIhzNW1o8wYSQ6An3MKR1GnInjw5u-9cCZ90uEGGYsPgU9QjklhLA-mE2gb93GdNDmcFM0ztjWQ23KEmq7qm1lqsbD2rjcupXJYOuLagHLYt0W2dfHZ6p8-NRdY0u76M7BSa5Kby729wC83t-9TB-Hs_nD03QyG2pK4mhINEGxyFmqcsFNRpjgDBEtVSSQFhGJU4SlpEhwETGMlJYcZ5jJPItRyhSNBuBml1s7u26Nb5JV4bedVbWtnwQrJOOEoUCv_9ClbV0V2m0Vl0xSKoO63SntrPfO5EntipVyXYJRsp04CRMnPxMHe7VPbNOwyEH-bhrAeAfei9J0_yclk_nzLvIbhDWE7w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1697969559</pqid></control><display><type>article</type><title>A survey of vitreous cell components performed using liquid‐based cytology</title><source>MEDLINE</source><source>Wiley Free Content</source><source>Wiley Online Library All Journals</source><creator>Narumi, Mari ; Nishitsuka, Koichi ; Yamakawa, Mitsunori ; Yamashita, Hidetoshi</creator><creatorcontrib>Narumi, Mari ; Nishitsuka, Koichi ; Yamakawa, Mitsunori ; Yamashita, Hidetoshi</creatorcontrib><description>Purpose
To confirm the efficacy of liquid‐based cytology (LBC) method in the observation of vitreous cells in various vitreoretinal diseases in human.
Methods
Vitreous fluid samples from 30 eyes were obtained by 23‐gauge 3‐port pars plana vitrectomy. After making three ports, we collected vitreous specimen from the core vitreous cavity without infusion. We divided the samples into a quiescent group and an active group based on clinical signs of inflammation. To confirm availability of LBC preparation slides for immunostaining, we also performed immunocytochemistry (ICC) for CD68, RPE65 and DEC‐205 (CD205) using LBC slides of 10 cell‐rich cases including retinal detachment and endophthalmitis.
Results
Using LBC method, small amounts of vitreous cells were observed efficiently. Vitreous cells were observed in inflammatory quiescent cases including macular pucker and macular hole. The number of vitreous cells increased significantly in the cases with clinically active inflammation (2297 versus 207 cells/ml, respectively, p < 0.01, Mann–Whitney U‐test). The ICC results showed the presence of CD68+ cells in all 10 cases. Large numbers of DEC‐205+ cells were observed in one case with infectious endophthalmitis. In the cases with retinal detachment, the predominant cell type was RPE65+. Neutrophils and lymphocytes were also observed.
Conclusions
The LBC method makes it possible to examine vitreous specimens easily and efficiently, facilitating the expedient diagnosis of vitreoretinal diseases, and the preparation slides are available for immunocytochemistry. This study also showed that vitreoretinal disease involves the migration of various types of cells including macrophages, neutrophils, lymphocytes, RPE65+ pigmented cells and DEC‐205+ cells.</description><identifier>ISSN: 1755-375X</identifier><identifier>EISSN: 1755-3768</identifier><identifier>DOI: 10.1111/aos.12623</identifier><identifier>PMID: 25752226</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Antigens, CD - metabolism ; Antigens, Differentiation, Myelomonocytic - metabolism ; Biomarkers - metabolism ; Cellular Structures - metabolism ; cis-trans-Isomerases - metabolism ; Cytological Techniques - methods ; Eye Diseases - diagnosis ; Female ; Humans ; immunocytochemistry ; Immunoenzyme Techniques ; Lectins, C-Type - metabolism ; liquid‐based cytology ; Male ; Middle Aged ; Minor Histocompatibility Antigens ; Ophthalmology ; Receptors, Cell Surface - metabolism ; Retinal Diseases - diagnosis ; Retrospective Studies ; Vitrectomy ; vitreoretinal diseases ; Vitreous Body - metabolism ; Vitreous Body - pathology ; vitreous cell</subject><ispartof>Acta ophthalmologica (Oxford, England), 2015-08, Vol.93 (5), p.e386-e390</ispartof><rights>2015 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd</rights><rights>2015 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2015 Acta Ophthalmologica Scandinavica Foundation</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5243-2c2048f6baf87ed2687602c9a380c8324b0199508783610ac971d169fd40b6a53</citedby><cites>FETCH-LOGICAL-c5243-2c2048f6baf87ed2687602c9a380c8324b0199508783610ac971d169fd40b6a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faos.12623$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faos.12623$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,27923,27924,45573,45574,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25752226$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Narumi, Mari</creatorcontrib><creatorcontrib>Nishitsuka, Koichi</creatorcontrib><creatorcontrib>Yamakawa, Mitsunori</creatorcontrib><creatorcontrib>Yamashita, Hidetoshi</creatorcontrib><title>A survey of vitreous cell components performed using liquid‐based cytology</title><title>Acta ophthalmologica (Oxford, England)</title><addtitle>Acta Ophthalmol</addtitle><description>Purpose
To confirm the efficacy of liquid‐based cytology (LBC) method in the observation of vitreous cells in various vitreoretinal diseases in human.
Methods
Vitreous fluid samples from 30 eyes were obtained by 23‐gauge 3‐port pars plana vitrectomy. After making three ports, we collected vitreous specimen from the core vitreous cavity without infusion. We divided the samples into a quiescent group and an active group based on clinical signs of inflammation. To confirm availability of LBC preparation slides for immunostaining, we also performed immunocytochemistry (ICC) for CD68, RPE65 and DEC‐205 (CD205) using LBC slides of 10 cell‐rich cases including retinal detachment and endophthalmitis.
Results
Using LBC method, small amounts of vitreous cells were observed efficiently. Vitreous cells were observed in inflammatory quiescent cases including macular pucker and macular hole. The number of vitreous cells increased significantly in the cases with clinically active inflammation (2297 versus 207 cells/ml, respectively, p < 0.01, Mann–Whitney U‐test). The ICC results showed the presence of CD68+ cells in all 10 cases. Large numbers of DEC‐205+ cells were observed in one case with infectious endophthalmitis. In the cases with retinal detachment, the predominant cell type was RPE65+. Neutrophils and lymphocytes were also observed.
Conclusions
The LBC method makes it possible to examine vitreous specimens easily and efficiently, facilitating the expedient diagnosis of vitreoretinal diseases, and the preparation slides are available for immunocytochemistry. This study also showed that vitreoretinal disease involves the migration of various types of cells including macrophages, neutrophils, lymphocytes, RPE65+ pigmented cells and DEC‐205+ cells.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antigens, CD - metabolism</subject><subject>Antigens, Differentiation, Myelomonocytic - metabolism</subject><subject>Biomarkers - metabolism</subject><subject>Cellular Structures - metabolism</subject><subject>cis-trans-Isomerases - metabolism</subject><subject>Cytological Techniques - methods</subject><subject>Eye Diseases - diagnosis</subject><subject>Female</subject><subject>Humans</subject><subject>immunocytochemistry</subject><subject>Immunoenzyme Techniques</subject><subject>Lectins, C-Type - metabolism</subject><subject>liquid‐based cytology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Minor Histocompatibility Antigens</subject><subject>Ophthalmology</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Retinal Diseases - diagnosis</subject><subject>Retrospective Studies</subject><subject>Vitrectomy</subject><subject>vitreoretinal diseases</subject><subject>Vitreous Body - metabolism</subject><subject>Vitreous Body - pathology</subject><subject>vitreous cell</subject><issn>1755-375X</issn><issn>1755-3768</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10M9KwzAABvAgipvTgy8gAS962Jakzb_jGP6DwQ4qeCtpmo6OrumSdtKbj-Az-iRmbu4gmEtC-PHx8QFwidEIhzNW1o8wYSQ6An3MKR1GnInjw5u-9cCZ90uEGGYsPgU9QjklhLA-mE2gb93GdNDmcFM0ztjWQ23KEmq7qm1lqsbD2rjcupXJYOuLagHLYt0W2dfHZ6p8-NRdY0u76M7BSa5Kby729wC83t-9TB-Hs_nD03QyG2pK4mhINEGxyFmqcsFNRpjgDBEtVSSQFhGJU4SlpEhwETGMlJYcZ5jJPItRyhSNBuBml1s7u26Nb5JV4bedVbWtnwQrJOOEoUCv_9ClbV0V2m0Vl0xSKoO63SntrPfO5EntipVyXYJRsp04CRMnPxMHe7VPbNOwyEH-bhrAeAfei9J0_yclk_nzLvIbhDWE7w</recordid><startdate>201508</startdate><enddate>201508</enddate><creator>Narumi, Mari</creator><creator>Nishitsuka, Koichi</creator><creator>Yamakawa, Mitsunori</creator><creator>Yamashita, Hidetoshi</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>201508</creationdate><title>A survey of vitreous cell components performed using liquid‐based cytology</title><author>Narumi, Mari ; Nishitsuka, Koichi ; Yamakawa, Mitsunori ; Yamashita, Hidetoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5243-2c2048f6baf87ed2687602c9a380c8324b0199508783610ac971d169fd40b6a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antigens, CD - metabolism</topic><topic>Antigens, Differentiation, Myelomonocytic - metabolism</topic><topic>Biomarkers - metabolism</topic><topic>Cellular Structures - metabolism</topic><topic>cis-trans-Isomerases - metabolism</topic><topic>Cytological Techniques - methods</topic><topic>Eye Diseases - diagnosis</topic><topic>Female</topic><topic>Humans</topic><topic>immunocytochemistry</topic><topic>Immunoenzyme Techniques</topic><topic>Lectins, C-Type - metabolism</topic><topic>liquid‐based cytology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Minor Histocompatibility Antigens</topic><topic>Ophthalmology</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Retinal Diseases - diagnosis</topic><topic>Retrospective Studies</topic><topic>Vitrectomy</topic><topic>vitreoretinal diseases</topic><topic>Vitreous Body - metabolism</topic><topic>Vitreous Body - pathology</topic><topic>vitreous cell</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Narumi, Mari</creatorcontrib><creatorcontrib>Nishitsuka, Koichi</creatorcontrib><creatorcontrib>Yamakawa, Mitsunori</creatorcontrib><creatorcontrib>Yamashita, Hidetoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta ophthalmologica (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Narumi, Mari</au><au>Nishitsuka, Koichi</au><au>Yamakawa, Mitsunori</au><au>Yamashita, Hidetoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A survey of vitreous cell components performed using liquid‐based cytology</atitle><jtitle>Acta ophthalmologica (Oxford, England)</jtitle><addtitle>Acta Ophthalmol</addtitle><date>2015-08</date><risdate>2015</risdate><volume>93</volume><issue>5</issue><spage>e386</spage><epage>e390</epage><pages>e386-e390</pages><issn>1755-375X</issn><eissn>1755-3768</eissn><abstract>Purpose
To confirm the efficacy of liquid‐based cytology (LBC) method in the observation of vitreous cells in various vitreoretinal diseases in human.
Methods
Vitreous fluid samples from 30 eyes were obtained by 23‐gauge 3‐port pars plana vitrectomy. After making three ports, we collected vitreous specimen from the core vitreous cavity without infusion. We divided the samples into a quiescent group and an active group based on clinical signs of inflammation. To confirm availability of LBC preparation slides for immunostaining, we also performed immunocytochemistry (ICC) for CD68, RPE65 and DEC‐205 (CD205) using LBC slides of 10 cell‐rich cases including retinal detachment and endophthalmitis.
Results
Using LBC method, small amounts of vitreous cells were observed efficiently. Vitreous cells were observed in inflammatory quiescent cases including macular pucker and macular hole. The number of vitreous cells increased significantly in the cases with clinically active inflammation (2297 versus 207 cells/ml, respectively, p < 0.01, Mann–Whitney U‐test). The ICC results showed the presence of CD68+ cells in all 10 cases. Large numbers of DEC‐205+ cells were observed in one case with infectious endophthalmitis. In the cases with retinal detachment, the predominant cell type was RPE65+. Neutrophils and lymphocytes were also observed.
Conclusions
The LBC method makes it possible to examine vitreous specimens easily and efficiently, facilitating the expedient diagnosis of vitreoretinal diseases, and the preparation slides are available for immunocytochemistry. This study also showed that vitreoretinal disease involves the migration of various types of cells including macrophages, neutrophils, lymphocytes, RPE65+ pigmented cells and DEC‐205+ cells.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>25752226</pmid><doi>10.1111/aos.12623</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Antigens, CD - metabolism Antigens, Differentiation, Myelomonocytic - metabolism Biomarkers - metabolism Cellular Structures - metabolism cis-trans-Isomerases - metabolism Cytological Techniques - methods Eye Diseases - diagnosis Female Humans immunocytochemistry Immunoenzyme Techniques Lectins, C-Type - metabolism liquid‐based cytology Male Middle Aged Minor Histocompatibility Antigens Ophthalmology Receptors, Cell Surface - metabolism Retinal Diseases - diagnosis Retrospective Studies Vitrectomy vitreoretinal diseases Vitreous Body - metabolism Vitreous Body - pathology vitreous cell |
| title | A survey of vitreous cell components performed using liquid‐based cytology |
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