Bcl-2 protein localizes to the chromosomes of mitotic nuclei and is correlated with the cell cycle in cultured epithelial cell lines
bcl-2 gene expression confers a survival advantage by preventing cells from entering apoptosis. In contrast to the previously described cytoplasmic localization of Bcl-2 in epithelial cells in vivo, in this study we have demonstrated, in a series of human epithelial cell lines, that Bcl-2 also local...
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| Veröffentlicht in: | Journal of cell science 1994-02, Vol.107 (2), p.363-371 |
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| container_title | Journal of cell science |
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| creator | QI-LONG LU HANBY, A. M NASSER HAJIBAGHERI, M. A GSCHMEISSNER, S. E PEI-JUAN LU TAYLOR-PAPADIMITRIOU, J KRAJEWSKI, S REED, J. C WRIGHT, N. A |
| description | bcl-2 gene expression confers a survival advantage by preventing cells from entering apoptosis. In contrast to the previously described cytoplasmic localization of Bcl-2 in epithelial cells in vivo, in this study we have demonstrated, in a series of human epithelial cell lines, that Bcl-2 also localizes to mitotic nuclei. Both immunocytochemical and immunoelectron microscopical examinations localize this protein to nuclei and in particular to chromosomes. Nuclear Bcl-2 expression in these cell lines is correlated with the cell cycle. There is relatively strong expression during mitosis, most intense during prophase and metaphase, declining in telophase and then the protein becomes undetectable soon after separation of the two daughter cells. The expression and distribution of Bcl-2 is influenced by treatment with excessive thymidine. These results indicate that Bcl-2 may protect the cells from apoptosis occurring during mitosis and suggest a possible role for the protein in cell immortalization. |
| doi_str_mv | 10.1242/jcs.107.2.363 |
| format | Article |
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M ; NASSER HAJIBAGHERI, M. A ; GSCHMEISSNER, S. E ; PEI-JUAN LU ; TAYLOR-PAPADIMITRIOU, J ; KRAJEWSKI, S ; REED, J. C ; WRIGHT, N. A</creator><creatorcontrib>QI-LONG LU ; HANBY, A. M ; NASSER HAJIBAGHERI, M. A ; GSCHMEISSNER, S. E ; PEI-JUAN LU ; TAYLOR-PAPADIMITRIOU, J ; KRAJEWSKI, S ; REED, J. C ; WRIGHT, N. A</creatorcontrib><description>bcl-2 gene expression confers a survival advantage by preventing cells from entering apoptosis. In contrast to the previously described cytoplasmic localization of Bcl-2 in epithelial cells in vivo, in this study we have demonstrated, in a series of human epithelial cell lines, that Bcl-2 also localizes to mitotic nuclei. Both immunocytochemical and immunoelectron microscopical examinations localize this protein to nuclei and in particular to chromosomes. Nuclear Bcl-2 expression in these cell lines is correlated with the cell cycle. There is relatively strong expression during mitosis, most intense during prophase and metaphase, declining in telophase and then the protein becomes undetectable soon after separation of the two daughter cells. The expression and distribution of Bcl-2 is influenced by treatment with excessive thymidine. These results indicate that Bcl-2 may protect the cells from apoptosis occurring during mitosis and suggest a possible role for the protein in cell immortalization.</description><identifier>ISSN: 0021-9533</identifier><identifier>EISSN: 1477-9137</identifier><identifier>DOI: 10.1242/jcs.107.2.363</identifier><identifier>PMID: 8207068</identifier><identifier>CODEN: JNCSAI</identifier><language>eng</language><publisher>Cambridge: Company of Biologists</publisher><subject>Biological and medical sciences ; Cell Cycle - genetics ; Cell Cycle - physiology ; Cell cycle, cell proliferation ; Cell Line ; Cell Nucleus - metabolism ; Cell Nucleus - ultrastructure ; Cell physiology ; Cell Transformation, Neoplastic - genetics ; Cell Transformation, Neoplastic - metabolism ; Cell Transformation, Neoplastic - ultrastructure ; Chromosomes, Human - metabolism ; Chromosomes, Human - ultrastructure ; Epithelial Cells ; Epithelium - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Neoplastic ; Humans ; Immunoblotting ; Immunohistochemistry ; Microscopy, Immunoelectron ; Mitosis - genetics ; Mitosis - physiology ; Molecular and cellular biology ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; Proto-Oncogene Proteins c-bcl-2</subject><ispartof>Journal of cell science, 1994-02, Vol.107 (2), p.363-371</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-c5fde7115d224e9597d633403a87d15119b6241c07a801ae28e29b57ef0a6f143</citedby><cites>FETCH-LOGICAL-c383t-c5fde7115d224e9597d633403a87d15119b6241c07a801ae28e29b57ef0a6f143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3665,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3975087$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8207068$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>QI-LONG LU</creatorcontrib><creatorcontrib>HANBY, A. 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Both immunocytochemical and immunoelectron microscopical examinations localize this protein to nuclei and in particular to chromosomes. Nuclear Bcl-2 expression in these cell lines is correlated with the cell cycle. There is relatively strong expression during mitosis, most intense during prophase and metaphase, declining in telophase and then the protein becomes undetectable soon after separation of the two daughter cells. The expression and distribution of Bcl-2 is influenced by treatment with excessive thymidine. 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Psychology</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Microscopy, Immunoelectron</subject><subject>Mitosis - genetics</subject><subject>Mitosis - physiology</subject><subject>Molecular and cellular biology</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Proto-Oncogene Proteins c-bcl-2</subject><issn>0021-9533</issn><issn>1477-9137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLxDAURoMo4_hYuhSyEHcd82h7m6UOvkBwo-uSSW-ZSNqMSYro2h9uZIZZ5ZLv3I_kEHLB2YKLUtx8mLjgDBZiIWt5QOa8BCgUl3BI5owJXqhKymNyEuMHYwyEghmZNYIBq5s5-b0zrhB0E3xCO1LnjXb2ByNNnqY1UrMOfvDRD_nK93SwySdr6DgZh5bqsaM2UuNDQKcTdvTLpvV2EZ2j5jtjNPeayaUp5Bw3GUBntdsSzo4Yz8hRr13E8915St4f7t-WT8XL6-Pz8valMLKRqTBV3yFwXnVClKgqBV0tZcmkbqDjFedqVYuSGwa6YVyjaFCoVQXYM133vJSn5Hrbm7_7OWFM7WDj_zP0iH6KLa9VA6KEDBZb0AQfY8C-3QQ76PDdctb-W2-z9TxDK9psPfOXu-JpNWC3p3eac361y3XMgvugR2PjHpMKKtaA_AOwe4tv</recordid><startdate>19940201</startdate><enddate>19940201</enddate><creator>QI-LONG LU</creator><creator>HANBY, A. 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Psychology</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Microscopy, Immunoelectron</topic><topic>Mitosis - genetics</topic><topic>Mitosis - physiology</topic><topic>Molecular and cellular biology</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Proto-Oncogene Proteins c-bcl-2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>QI-LONG LU</creatorcontrib><creatorcontrib>HANBY, A. M</creatorcontrib><creatorcontrib>NASSER HAJIBAGHERI, M. A</creatorcontrib><creatorcontrib>GSCHMEISSNER, S. E</creatorcontrib><creatorcontrib>PEI-JUAN LU</creatorcontrib><creatorcontrib>TAYLOR-PAPADIMITRIOU, J</creatorcontrib><creatorcontrib>KRAJEWSKI, S</creatorcontrib><creatorcontrib>REED, J. C</creatorcontrib><creatorcontrib>WRIGHT, N. 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| ispartof | Journal of cell science, 1994-02, Vol.107 (2), p.363-371 |
| issn | 0021-9533 1477-9137 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Company of Biologists |
| subjects | Biological and medical sciences Cell Cycle - genetics Cell Cycle - physiology Cell cycle, cell proliferation Cell Line Cell Nucleus - metabolism Cell Nucleus - ultrastructure Cell physiology Cell Transformation, Neoplastic - genetics Cell Transformation, Neoplastic - metabolism Cell Transformation, Neoplastic - ultrastructure Chromosomes, Human - metabolism Chromosomes, Human - ultrastructure Epithelial Cells Epithelium - metabolism Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Neoplastic Humans Immunoblotting Immunohistochemistry Microscopy, Immunoelectron Mitosis - genetics Mitosis - physiology Molecular and cellular biology Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-bcl-2 |
| title | Bcl-2 protein localizes to the chromosomes of mitotic nuclei and is correlated with the cell cycle in cultured epithelial cell lines |
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