Detection of Membrane-Bound HLA-G Translated Products with a Specific Monoclonal Antibody

A monomorphic anti-HLA-G monoclonal antibody (mAb) was obtained by immunization of HLA-B27/human β2-microglobulin double-transgenic mice with transfected murine L cells expressing both HLA-G and human β2-microglobulin. This mAb, designated BFL.1, specifically recognizes, by flow cytometry analysis,...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1995-10, Vol.92 (22), p.10292-10296
Hauptverfasser:	Bensussan, Armand, Mansur, Indra-Gusti, Mallet, Valerie, Rodriguez, Anne-Marie, Girr, Maryse, Weiss, Elisabeth H., Brem, Gottfried, Boumsell, Laurence, Gluckman, Elaine, Dausset, Jean, Carosella, Edgardo, Le Bouteiller, Philippe
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Antibody Specificity Cell Line Cell lines Cell Membrane - immunology Cellular biology Choriocarcinoma Cytometry Female Flow Cytometry Fluorescent Antibody Technique, Indirect Histocompatibility Antigens Class I - analysis Histocompatibility Antigens Class I - biosynthesis HLA antigens HLA Antigens - analysis HLA Antigens - biosynthesis HLA-G Antigens Humans L cells Mice Mice, Inbred Strains Mice, Transgenic Molecules PC12 cells Placenta Pregnancy Pregnancy Trimester, First Protein isoforms Recombinant Proteins - analysis Recombinant Proteins - biosynthesis Trophoblasts Trophoblasts - immunology Tumor Cells, Cultured
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