Nuclear accumulation of p53 in response to treatment with DNA-damaging agents
A number of agents which damage DNA also trigger the nuclear accumulation of the tumor suppressor protein p53. Here we show the correlation with different p53 detection methods. As an example we investigated the effects of the cancer therapy drug mitomycin C on different mammalian cell lines. Our fi...
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| Veröffentlicht in: | Toxicology letters 1994-06, Vol.72 (1), p.43-52 |
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| container_title | Toxicology letters |
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| creator | Hess, Ralf Plaumann, Bettina Lutum, Annegret Schulze Haessler, Christel Heinz, Barbara Fritsche, Michael Brandner, Gerhard |
| description | A number of agents which damage DNA also trigger the nuclear accumulation of the tumor suppressor protein p53. Here we show the correlation with different p53 detection methods. As an example we investigated the effects of the cancer therapy drug mitomycin C on different mammalian cell lines. Our findings demonstrate that either the immunofluorescence techniques (indirect immunofluorescence staining or flow cytometric analysis) or ELISA or immunoblot assays are useful methods in detecting p53 accumulation. Simultaneously we measured DNA damage with the terminal deoxynucleotidyl transferase assay. Compatible data were obtained. Thus p53 accumulation may be used as indicator of DNA injury. |
| doi_str_mv | 10.1016/0378-4274(94)90008-6 |
| format | Article |
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Here we show the correlation with different p53 detection methods. As an example we investigated the effects of the cancer therapy drug mitomycin C on different mammalian cell lines. Our findings demonstrate that either the immunofluorescence techniques (indirect immunofluorescence staining or flow cytometric analysis) or ELISA or immunoblot assays are useful methods in detecting p53 accumulation. Simultaneously we measured DNA damage with the terminal deoxynucleotidyl transferase assay. Compatible data were obtained. Thus p53 accumulation may be used as indicator of DNA injury.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Cell Nucleus - metabolism</subject><subject>Cells, Cultured</subject><subject>Cercopithecus aethiops</subject><subject>Chemical agents</subject><subject>DNA Damage</subject><subject>DNA Nucleotidylexotransferase - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>Immunoblotting</subject><subject>Immunochemistry</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C3H</subject><subject>Mitomycin - toxicity</subject><subject>p53</subject><subject>p53 nuclear accumulation</subject><subject>Tumor Suppressor Protein p53 - analysis</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><subject>Tumors</subject><issn>0378-4274</issn><issn>1879-3169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLBDEMgIsouj7-gUIPInoYbaedTnsRFt_g46LnUjvpWpnH2nYU_71ddtmjEAgkX0LyIXRIyTklVFwQVsuClzU_VfxMEUJkITbQhMpaFYwKtYkma2QH7cb4mRnBRbWNtmVJSlVVE_T0PNoWTMDG2rEbW5P80OPB4XnFsO9xgDgf-gg4DTgFMKmDPuEfnz7w9fO0aExnZr6fYTPL9biPtpxpIxys8h56u715vbovHl_uHq6mj4XltE6FpAQc48SxunFGEFYxZ6VjtHq3SjIQ3KhK2aYUhDaltAyU4k5xwURpieJsD50s987D8DVCTLrz0ULbmh6GMer8vaRC0gzyJWjDEGMAp-fBdyb8akr0wqJeKNILRVrlWFjUIo8drfaP7x0066GVttw_XvVNtKZ1wfTWxzXGafZcy4xdLjHILr49BB2th95C4wPYpJvB_3_HH61pjEU</recordid><startdate>19940601</startdate><enddate>19940601</enddate><creator>Hess, Ralf</creator><creator>Plaumann, Bettina</creator><creator>Lutum, Annegret Schulze</creator><creator>Haessler, Christel</creator><creator>Heinz, Barbara</creator><creator>Fritsche, Michael</creator><creator>Brandner, Gerhard</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19940601</creationdate><title>Nuclear accumulation of p53 in response to treatment with DNA-damaging agents</title><author>Hess, Ralf ; Plaumann, Bettina ; Lutum, Annegret Schulze ; Haessler, Christel ; Heinz, Barbara ; Fritsche, Michael ; Brandner, Gerhard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-810ef340f37dfa60353fc8f315bc983e64a959cd2601d28c3e994f946362c0943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Cell Nucleus - metabolism</topic><topic>Cells, Cultured</topic><topic>Cercopithecus aethiops</topic><topic>Chemical agents</topic><topic>DNA Damage</topic><topic>DNA Nucleotidylexotransferase - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique</topic><topic>Immunoblotting</topic><topic>Immunochemistry</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C3H</topic><topic>Mitomycin - toxicity</topic><topic>p53</topic><topic>p53 nuclear accumulation</topic><topic>Tumor Suppressor Protein p53 - analysis</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hess, Ralf</creatorcontrib><creatorcontrib>Plaumann, Bettina</creatorcontrib><creatorcontrib>Lutum, Annegret Schulze</creatorcontrib><creatorcontrib>Haessler, Christel</creatorcontrib><creatorcontrib>Heinz, Barbara</creatorcontrib><creatorcontrib>Fritsche, Michael</creatorcontrib><creatorcontrib>Brandner, Gerhard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hess, Ralf</au><au>Plaumann, Bettina</au><au>Lutum, Annegret Schulze</au><au>Haessler, Christel</au><au>Heinz, Barbara</au><au>Fritsche, Michael</au><au>Brandner, Gerhard</au><au>Alink, GM</au><au>Hadnagy, W (eds)</au><au>Seemayer, NH</au><au>Heussen, GAH</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear accumulation of p53 in response to treatment with DNA-damaging agents</atitle><jtitle>Toxicology letters</jtitle><addtitle>Toxicol Lett</addtitle><date>1994-06-01</date><risdate>1994</risdate><volume>72</volume><issue>1</issue><spage>43</spage><epage>52</epage><pages>43-52</pages><issn>0378-4274</issn><eissn>1879-3169</eissn><coden>TOLED5</coden><abstract>A number of agents which damage DNA also trigger the nuclear accumulation of the tumor suppressor protein p53. Here we show the correlation with different p53 detection methods. As an example we investigated the effects of the cancer therapy drug mitomycin C on different mammalian cell lines. Our findings demonstrate that either the immunofluorescence techniques (indirect immunofluorescence staining or flow cytometric analysis) or ELISA or immunoblot assays are useful methods in detecting p53 accumulation. Simultaneously we measured DNA damage with the terminal deoxynucleotidyl transferase assay. Compatible data were obtained. Thus p53 accumulation may be used as indicator of DNA injury.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>8202955</pmid><doi>10.1016/0378-4274(94)90008-6</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0378-4274 |
| ispartof | Toxicology letters, 1994-06, Vol.72 (1), p.43-52 |
| issn | 0378-4274 1879-3169 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 3T3 Cells Animals Biological and medical sciences Carcinogenesis, carcinogens and anticarcinogens Cell Nucleus - metabolism Cells, Cultured Cercopithecus aethiops Chemical agents DNA Damage DNA Nucleotidylexotransferase - metabolism Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorescent Antibody Technique Immunoblotting Immunochemistry Medical sciences Mice Mice, Inbred BALB C Mice, Inbred C3H Mitomycin - toxicity p53 p53 nuclear accumulation Tumor Suppressor Protein p53 - analysis Tumor Suppressor Protein p53 - metabolism Tumors |
| title | Nuclear accumulation of p53 in response to treatment with DNA-damaging agents |
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