Easy-to-perform modified Elek test to identify Shiga-like toxin-producing diarrhoeogenic Escherichia coli
We determined whether Shiga-like toxin I (SLT-I) -producing diarrhoeogenic Escherichia coli could be detected by a modified Elek test. The test (SLT Elek test) is based on the principle of the Elek test and the Ouchterlony double-gel diffusion. The development of the SLT Elek test was preceded by a...
Gespeichert in:
| Veröffentlicht in: | Research in microbiology 1994, Vol.145 (4), p.333-340 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 340 |
|---|---|
| container_issue | 4 |
| container_start_page | 333 |
| container_title | Research in microbiology |
| container_volume | 145 |
| creator | Germani, Y. Degaud, E. Desperrier, J.M. |
| description | We determined whether Shiga-like toxin I (SLT-I) -producing diarrhoeogenic
Escherichia coli could be detected by a modified Elek test. The test (SLT Elek test) is based on the principle of the Elek test and the Ouchterlony double-gel diffusion. The development of the SLT Elek test was preceded by a preliminary study; the purpose of the latter was to establish whether a simplified purification procedure of SLT-I (involving bacterial sonic extract, “Affi-Gel Blue” chromatography and anion- and cation-exchange liquid chromatography) could be employed in the preparation of rabbit antisera to SLT-I. SLT-I-specific antisera were obtained after adsorption of sera with bacterial sonic extract from non-toxigenic
E. coli. A total of 135 strains of
E. coli were tested by the SLT Elek test (100 SLT-I-negative and 35 SLT-I-positive). The results of the SLT Elek test and the Vero cell test correlated well: 30 strains gave positive results and 100 strains gave negative results in both tests. Only 5 strains gave discrepant results: they were weakly positive in the Vero cell assay, whereas 3 gave borderline reactions and 2 were negative in the SLT Elek test. Positive predictive value was 1, negative predictive value was 0.98; the SLT Elek test was 91 % sensitive and 100 % specific. |
| doi_str_mv | 10.1016/0923-2508(94)90188-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16978501</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0923250894901880</els_id><sourcerecordid>16978501</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3780-34e0e0e441499dbc1ae0125659dc14048ae3f3b4ebe84f6a0b3512fcf8c1a9983</originalsourceid><addsrcrecordid>eNp9kMGKFDEQhoMo6-zqGyjkIKKHaNJJdycXQZZZFRY8qOeQTioz5XZ3xqRHnLc34wxzlDoUVH1V_HyEvBD8neCie89NI1nTcv3GqLeGC60Zf0RWou8M60UjH5PVBXlKrkv5yblo-15dkavemL5T3Yrg2pUDWxLbQY4pT3RKASNCoOsRHugCZaFLohhgXjAe6Lctbhwb8QHq-A_ObJdT2HucNzSgy3mbIG1gRk_XxW8ho9-ioz6N-Iw8iW4s8Pzcb8iPu_X328_s_uunL7cf75mXveZMKuC1lBLKmDB44YCLpu1aE7xQXGkHMspBwQBaxc7xQbaiiT7qihqj5Q15ffpbk_3a1_x2wuJhHN0MaV-s6EyvWy4qqE6gz6mUDNHuMk4uH6zg9mjYHvXZoz5rlP1n2PJ69vL8fz9MEC5HZ6V1_-q8d8W7MWY3eywXTAktOW8q9uGEQXXxGyHb4hFmDwEz-MWGhP_P8Rd4xJg3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16978501</pqid></control><display><type>article</type><title>Easy-to-perform modified Elek test to identify Shiga-like toxin-producing diarrhoeogenic Escherichia coli</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Germani, Y. ; Degaud, E. ; Desperrier, J.M.</creator><creatorcontrib>Germani, Y. ; Degaud, E. ; Desperrier, J.M.</creatorcontrib><description>We determined whether Shiga-like toxin I (SLT-I) -producing diarrhoeogenic
Escherichia coli could be detected by a modified Elek test. The test (SLT Elek test) is based on the principle of the Elek test and the Ouchterlony double-gel diffusion. The development of the SLT Elek test was preceded by a preliminary study; the purpose of the latter was to establish whether a simplified purification procedure of SLT-I (involving bacterial sonic extract, “Affi-Gel Blue” chromatography and anion- and cation-exchange liquid chromatography) could be employed in the preparation of rabbit antisera to SLT-I. SLT-I-specific antisera were obtained after adsorption of sera with bacterial sonic extract from non-toxigenic
E. coli. A total of 135 strains of
E. coli were tested by the SLT Elek test (100 SLT-I-negative and 35 SLT-I-positive). The results of the SLT Elek test and the Vero cell test correlated well: 30 strains gave positive results and 100 strains gave negative results in both tests. Only 5 strains gave discrepant results: they were weakly positive in the Vero cell assay, whereas 3 gave borderline reactions and 2 were negative in the SLT Elek test. Positive predictive value was 1, negative predictive value was 0.98; the SLT Elek test was 91 % sensitive and 100 % specific.</description><identifier>ISSN: 0923-2508</identifier><identifier>EISSN: 1769-7123</identifier><identifier>DOI: 10.1016/0923-2508(94)90188-0</identifier><identifier>PMID: 7997646</identifier><language>eng</language><publisher>Paris: Elsevier SAS</publisher><subject>Bacterial Toxins - biosynthesis ; Bacteriological methods and techniques used in bacteriology ; Bacteriological Techniques ; Bacteriology ; Biological and medical sciences ; Chromatography, Gel ; Chromatography, Ion Exchange ; Diarrhea - microbiology ; Diarrhoea, Escherichia coli, Toxigenesis ; Elek test, Identification, Shiga-like toxin I ; Escherichia coli ; Escherichia coli - isolation & purification ; Escherichia coli - metabolism ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunodiffusion ; In Vitro Techniques ; Microbiology ; Shiga Toxin 1</subject><ispartof>Research in microbiology, 1994, Vol.145 (4), p.333-340</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3780-34e0e0e441499dbc1ae0125659dc14048ae3f3b4ebe84f6a0b3512fcf8c1a9983</citedby><cites>FETCH-LOGICAL-c3780-34e0e0e441499dbc1ae0125659dc14048ae3f3b4ebe84f6a0b3512fcf8c1a9983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0923250894901880$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4183002$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7997646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Germani, Y.</creatorcontrib><creatorcontrib>Degaud, E.</creatorcontrib><creatorcontrib>Desperrier, J.M.</creatorcontrib><title>Easy-to-perform modified Elek test to identify Shiga-like toxin-producing diarrhoeogenic Escherichia coli</title><title>Research in microbiology</title><addtitle>Res Microbiol</addtitle><description>We determined whether Shiga-like toxin I (SLT-I) -producing diarrhoeogenic
Escherichia coli could be detected by a modified Elek test. The test (SLT Elek test) is based on the principle of the Elek test and the Ouchterlony double-gel diffusion. The development of the SLT Elek test was preceded by a preliminary study; the purpose of the latter was to establish whether a simplified purification procedure of SLT-I (involving bacterial sonic extract, “Affi-Gel Blue” chromatography and anion- and cation-exchange liquid chromatography) could be employed in the preparation of rabbit antisera to SLT-I. SLT-I-specific antisera were obtained after adsorption of sera with bacterial sonic extract from non-toxigenic
E. coli. A total of 135 strains of
E. coli were tested by the SLT Elek test (100 SLT-I-negative and 35 SLT-I-positive). The results of the SLT Elek test and the Vero cell test correlated well: 30 strains gave positive results and 100 strains gave negative results in both tests. Only 5 strains gave discrepant results: they were weakly positive in the Vero cell assay, whereas 3 gave borderline reactions and 2 were negative in the SLT Elek test. Positive predictive value was 1, negative predictive value was 0.98; the SLT Elek test was 91 % sensitive and 100 % specific.</description><subject>Bacterial Toxins - biosynthesis</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriological Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Ion Exchange</subject><subject>Diarrhea - microbiology</subject><subject>Diarrhoea, Escherichia coli, Toxigenesis</subject><subject>Elek test, Identification, Shiga-like toxin I</subject><subject>Escherichia coli</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunodiffusion</subject><subject>In Vitro Techniques</subject><subject>Microbiology</subject><subject>Shiga Toxin 1</subject><issn>0923-2508</issn><issn>1769-7123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMGKFDEQhoMo6-zqGyjkIKKHaNJJdycXQZZZFRY8qOeQTioz5XZ3xqRHnLc34wxzlDoUVH1V_HyEvBD8neCie89NI1nTcv3GqLeGC60Zf0RWou8M60UjH5PVBXlKrkv5yblo-15dkavemL5T3Yrg2pUDWxLbQY4pT3RKASNCoOsRHugCZaFLohhgXjAe6Lctbhwb8QHq-A_ObJdT2HucNzSgy3mbIG1gRk_XxW8ho9-ioz6N-Iw8iW4s8Pzcb8iPu_X328_s_uunL7cf75mXveZMKuC1lBLKmDB44YCLpu1aE7xQXGkHMspBwQBaxc7xQbaiiT7qihqj5Q15ffpbk_3a1_x2wuJhHN0MaV-s6EyvWy4qqE6gz6mUDNHuMk4uH6zg9mjYHvXZoz5rlP1n2PJ69vL8fz9MEC5HZ6V1_-q8d8W7MWY3eywXTAktOW8q9uGEQXXxGyHb4hFmDwEz-MWGhP_P8Rd4xJg3</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Germani, Y.</creator><creator>Degaud, E.</creator><creator>Desperrier, J.M.</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>1994</creationdate><title>Easy-to-perform modified Elek test to identify Shiga-like toxin-producing diarrhoeogenic Escherichia coli</title><author>Germani, Y. ; Degaud, E. ; Desperrier, J.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3780-34e0e0e441499dbc1ae0125659dc14048ae3f3b4ebe84f6a0b3512fcf8c1a9983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Bacterial Toxins - biosynthesis</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriological Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Gel</topic><topic>Chromatography, Ion Exchange</topic><topic>Diarrhea - microbiology</topic><topic>Diarrhoea, Escherichia coli, Toxigenesis</topic><topic>Elek test, Identification, Shiga-like toxin I</topic><topic>Escherichia coli</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunodiffusion</topic><topic>In Vitro Techniques</topic><topic>Microbiology</topic><topic>Shiga Toxin 1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Germani, Y.</creatorcontrib><creatorcontrib>Degaud, E.</creatorcontrib><creatorcontrib>Desperrier, J.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Research in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Germani, Y.</au><au>Degaud, E.</au><au>Desperrier, J.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Easy-to-perform modified Elek test to identify Shiga-like toxin-producing diarrhoeogenic Escherichia coli</atitle><jtitle>Research in microbiology</jtitle><addtitle>Res Microbiol</addtitle><date>1994</date><risdate>1994</risdate><volume>145</volume><issue>4</issue><spage>333</spage><epage>340</epage><pages>333-340</pages><issn>0923-2508</issn><eissn>1769-7123</eissn><abstract>We determined whether Shiga-like toxin I (SLT-I) -producing diarrhoeogenic
Escherichia coli could be detected by a modified Elek test. The test (SLT Elek test) is based on the principle of the Elek test and the Ouchterlony double-gel diffusion. The development of the SLT Elek test was preceded by a preliminary study; the purpose of the latter was to establish whether a simplified purification procedure of SLT-I (involving bacterial sonic extract, “Affi-Gel Blue” chromatography and anion- and cation-exchange liquid chromatography) could be employed in the preparation of rabbit antisera to SLT-I. SLT-I-specific antisera were obtained after adsorption of sera with bacterial sonic extract from non-toxigenic
E. coli. A total of 135 strains of
E. coli were tested by the SLT Elek test (100 SLT-I-negative and 35 SLT-I-positive). The results of the SLT Elek test and the Vero cell test correlated well: 30 strains gave positive results and 100 strains gave negative results in both tests. Only 5 strains gave discrepant results: they were weakly positive in the Vero cell assay, whereas 3 gave borderline reactions and 2 were negative in the SLT Elek test. Positive predictive value was 1, negative predictive value was 0.98; the SLT Elek test was 91 % sensitive and 100 % specific.</abstract><cop>Paris</cop><pub>Elsevier SAS</pub><pmid>7997646</pmid><doi>10.1016/0923-2508(94)90188-0</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0923-2508 |
| ispartof | Research in microbiology, 1994, Vol.145 (4), p.333-340 |
| issn | 0923-2508 1769-7123 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16978501 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacterial Toxins - biosynthesis Bacteriological methods and techniques used in bacteriology Bacteriological Techniques Bacteriology Biological and medical sciences Chromatography, Gel Chromatography, Ion Exchange Diarrhea - microbiology Diarrhoea, Escherichia coli, Toxigenesis Elek test, Identification, Shiga-like toxin I Escherichia coli Escherichia coli - isolation & purification Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Humans Immunodiffusion In Vitro Techniques Microbiology Shiga Toxin 1 |
| title | Easy-to-perform modified Elek test to identify Shiga-like toxin-producing diarrhoeogenic Escherichia coli |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T21%3A32%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Easy-to-perform%20modified%20Elek%20test%20to%20identify%20Shiga-like%20toxin-producing%20diarrhoeogenic%20Escherichia%20coli&rft.jtitle=Research%20in%20microbiology&rft.au=Germani,%20Y.&rft.date=1994&rft.volume=145&rft.issue=4&rft.spage=333&rft.epage=340&rft.pages=333-340&rft.issn=0923-2508&rft.eissn=1769-7123&rft_id=info:doi/10.1016/0923-2508(94)90188-0&rft_dat=%3Cproquest_cross%3E16978501%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16978501&rft_id=info:pmid/7997646&rft_els_id=0923250894901880&rfr_iscdi=true |