A Method for Determination of Lipid-Bound Sialic Acid After Chromatographic Isolation of Brain Gangliosides

A Method for Determination of Lipid-Bound Sialic Acid After Chromatographic Isolation of Brain Gangliosides

The lipid-bound sialic acid determination gives an indirect idea of the ganglioside composition. An attempt at adapting the 'resorcinol method' of Svennerholm using thin-layer chromatography is described in this article. An aliquot of a lipid extract from bovine brain was chromatographed a...

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Veröffentlicht in:Journal of liquid chromatography 1994-08, Vol.17 (13), p.2871-2879
Hauptverfasser: Ilinov, Peter P., Deleva, Denislava D., Zaprianova, Emilia T., Dimov, Svetoslav I.
Format: Artikel
Sprache:eng
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Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Lipids
Other biological molecules
Sphingolipids
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container_end_page 2879
container_issue 13
container_start_page 2871
container_title Journal of liquid chromatography
container_volume 17
creator Ilinov, Peter P.
Deleva, Denislava D.
Zaprianova, Emilia T.
Dimov, Svetoslav I.
description The lipid-bound sialic acid determination gives an indirect idea of the ganglioside composition. An attempt at adapting the 'resorcinol method' of Svennerholm using thin-layer chromatography is described in this article. An aliquot of a lipid extract from bovine brain was chromatographed and the ganglioside carrying area was visualised. The sorbent was scrapped and eluted. The sialic acid was hydrolysed with a mixture of resorcinol/hydrochloric acid and the color product formed was extracted with butanol/butylacetate. The color intensity was measured at 580 nm. It was found that the lipid-bound sialic acid in bovine brain was about 780 μg/g fresh tissue. The recovery data calculated by means of a standart solution were 97-102%. This method is particularly useful for some routine diagnostic studies.
doi_str_mv 10.1080/10826079408013505
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subjects Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Lipids
Other biological molecules
Sphingolipids
title A Method for Determination of Lipid-Bound Sialic Acid After Chromatographic Isolation of Brain Gangliosides
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