New insights into the lymphovascular microanatomy of the colon and the risk of metastases in pT1 colorectal cancer obtained with quantitative methods and three-dimensional digital reconstruction
Aims UK faecal occult blood test screening has tripled the proportion of pT1 colorectal cancers. The risk of metastasis is predicted by depth of invasion, suggesting that access to deep lymphovascular vessels is important. The aim of this study was to quantify the distribution and size of the submuc...
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| Veröffentlicht in: | Histopathology 2015-08, Vol.67 (2), p.167-175 |
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| creator | Brown, Peter J Toh, Eu-Wing Smith, Katherine J E Jones, Pamela Treanor, Darren Magee, Derek Burke, Dermot Quirke, Phil |
| description | Aims
UK faecal occult blood test screening has tripled the proportion of pT1 colorectal cancers. The risk of metastasis is predicted by depth of invasion, suggesting that access to deep lymphovascular vessels is important. The aim of this study was to quantify the distribution and size of the submucosal vasculature, and generate a novel three‐dimensional (3D) model to validate the findings.
Methods and results
Thirty samples of normal large bowel wall were immunostained with CD31, a vascular endothelium marker, to identify blood vessels, which were quantified and digitally analysed for their number, circumference, area and diameter in the deep mucosa and submucosa (Sm1, Sm2, and Sm3). The model required serial sections, a double immunostain (using CD31 and D2‐40), and 3D reconstruction. Significant differences were shown between submucosal layers in the number, circumference and area of vessels (P |
| doi_str_mv | 10.1111/his.12639 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1697218473</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1697218473</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4269-cdece918dd9eb49dbc456489e613ce0d50e0ac5257e8f906d1000737760956763</originalsourceid><addsrcrecordid>eNp1kdFuFCEUhonR2LV64QsYEm_0YloYBlguTaPdJk1NbI2XhIWzHdqZYQtM1309n0xmd9sLE4EEyPn-Pxx-hN5TckLLOG19OqG1YOoFmlEmeFVzrl6iGWFEVYQKeYTepHRHCJWsrl-jo1LnUtVshv5cwQb7IfnbNqdyyAHnFnC37ddteDTJjp2JuPc2BjOYHPotDqsdYkMXBmwGt7tFn-6nSg_ZpLJgMsPrG7rjIthsOmzNYCHisMzGD-DwxucWP4xmyD6b7B9hkrfBpYNtBKic76E8LwxF7_ytn3yKXRhSjqPNpfAWvVqZLsG7w36Mfn77enO2qC6_n1-cfbmsbFMLVVkHFhSdO6dg2Si3tA0XzVyBoMwCcZwAMZbXXMJ8pYhwlBAimZSCKC6kYMfo0953HcPDCCnr3icLXWcGCGPSVChZ03kjWUE__oPehTGWFnaUIE2ZpFCf91T53JQirPQ6-t7EraZET8HqEqzeBVvYDwfHcdmDeyafkizA6R7Y-A62_3fSi4vrJ8tqr_Apw-9nhYn3WpS-uf51da6va04XzQ-mGfsLwgfAJA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1696040400</pqid></control><display><type>article</type><title>New insights into the lymphovascular microanatomy of the colon and the risk of metastases in pT1 colorectal cancer obtained with quantitative methods and three-dimensional digital reconstruction</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Brown, Peter J ; Toh, Eu-Wing ; Smith, Katherine J E ; Jones, Pamela ; Treanor, Darren ; Magee, Derek ; Burke, Dermot ; Quirke, Phil</creator><creatorcontrib>Brown, Peter J ; Toh, Eu-Wing ; Smith, Katherine J E ; Jones, Pamela ; Treanor, Darren ; Magee, Derek ; Burke, Dermot ; Quirke, Phil</creatorcontrib><description>Aims
UK faecal occult blood test screening has tripled the proportion of pT1 colorectal cancers. The risk of metastasis is predicted by depth of invasion, suggesting that access to deep lymphovascular vessels is important. The aim of this study was to quantify the distribution and size of the submucosal vasculature, and generate a novel three‐dimensional (3D) model to validate the findings.
Methods and results
Thirty samples of normal large bowel wall were immunostained with CD31, a vascular endothelium marker, to identify blood vessels, which were quantified and digitally analysed for their number, circumference, area and diameter in the deep mucosa and submucosa (Sm1, Sm2, and Sm3). The model required serial sections, a double immunostain (using CD31 and D2‐40), and 3D reconstruction. Significant differences were shown between submucosal layers in the number, circumference and area of vessels (P < 0.001). Blood vessels were most numerous in the mucosa (11.79 vessels/0.2 mm2) but smaller [median area of 247 μm2, interquartile range (IQR) 162–373 μm2] than in Sm2, where they were fewer in number (6.92 vessels/0.2 mm2) but considerably larger (2086 μm2, IQR 1007–4784 μm2). The 3D model generated novel observations on lymphovascular structures.
Conclusions
The number and size of blood vessels do not increase with depth of submucosa, as hypothesized. The distribution of vessels suggests that we should investigate the area or volume of submucosal invasion rather than the depth.</description><identifier>ISSN: 0309-0167</identifier><identifier>EISSN: 1365-2559</identifier><identifier>DOI: 10.1111/his.12639</identifier><identifier>PMID: 25557923</identifier><identifier>CODEN: HISTDD</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Aged ; Biomarkers, Tumor - metabolism ; CD31 ; colorectal cancer ; Colorectal Neoplasms - blood supply ; Colorectal Neoplasms - pathology ; Computer Simulation ; Early Detection of Cancer - methods ; Endothelium, Vascular - metabolism ; Female ; Humans ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional ; Intestinal Mucosa - pathology ; Lymph Nodes - pathology ; Lymphatic Metastasis ; Male ; microvessel anatomy ; Middle Aged ; Neoplasm Invasiveness ; Neovascularization, Pathologic - metabolism ; Neovascularization, Pathologic - pathology ; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism ; Risk Factors ; submucosal plexus</subject><ispartof>Histopathology, 2015-08, Vol.67 (2), p.167-175</ispartof><rights>2015 John Wiley & Sons Ltd</rights><rights>2015 John Wiley & Sons Ltd.</rights><rights>Copyright © 2015 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4269-cdece918dd9eb49dbc456489e613ce0d50e0ac5257e8f906d1000737760956763</citedby><cites>FETCH-LOGICAL-c4269-cdece918dd9eb49dbc456489e613ce0d50e0ac5257e8f906d1000737760956763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fhis.12639$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fhis.12639$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25557923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, Peter J</creatorcontrib><creatorcontrib>Toh, Eu-Wing</creatorcontrib><creatorcontrib>Smith, Katherine J E</creatorcontrib><creatorcontrib>Jones, Pamela</creatorcontrib><creatorcontrib>Treanor, Darren</creatorcontrib><creatorcontrib>Magee, Derek</creatorcontrib><creatorcontrib>Burke, Dermot</creatorcontrib><creatorcontrib>Quirke, Phil</creatorcontrib><title>New insights into the lymphovascular microanatomy of the colon and the risk of metastases in pT1 colorectal cancer obtained with quantitative methods and three-dimensional digital reconstruction</title><title>Histopathology</title><addtitle>Histopathology</addtitle><description>Aims
UK faecal occult blood test screening has tripled the proportion of pT1 colorectal cancers. The risk of metastasis is predicted by depth of invasion, suggesting that access to deep lymphovascular vessels is important. The aim of this study was to quantify the distribution and size of the submucosal vasculature, and generate a novel three‐dimensional (3D) model to validate the findings.
Methods and results
Thirty samples of normal large bowel wall were immunostained with CD31, a vascular endothelium marker, to identify blood vessels, which were quantified and digitally analysed for their number, circumference, area and diameter in the deep mucosa and submucosa (Sm1, Sm2, and Sm3). The model required serial sections, a double immunostain (using CD31 and D2‐40), and 3D reconstruction. Significant differences were shown between submucosal layers in the number, circumference and area of vessels (P < 0.001). Blood vessels were most numerous in the mucosa (11.79 vessels/0.2 mm2) but smaller [median area of 247 μm2, interquartile range (IQR) 162–373 μm2] than in Sm2, where they were fewer in number (6.92 vessels/0.2 mm2) but considerably larger (2086 μm2, IQR 1007–4784 μm2). The 3D model generated novel observations on lymphovascular structures.
Conclusions
The number and size of blood vessels do not increase with depth of submucosa, as hypothesized. The distribution of vessels suggests that we should investigate the area or volume of submucosal invasion rather than the depth.</description><subject>Aged</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>CD31</subject><subject>colorectal cancer</subject><subject>Colorectal Neoplasms - blood supply</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Computer Simulation</subject><subject>Early Detection of Cancer - methods</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted</subject><subject>Imaging, Three-Dimensional</subject><subject>Intestinal Mucosa - pathology</subject><subject>Lymph Nodes - pathology</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>microvessel anatomy</subject><subject>Middle Aged</subject><subject>Neoplasm Invasiveness</subject><subject>Neovascularization, Pathologic - metabolism</subject><subject>Neovascularization, Pathologic - pathology</subject><subject>Platelet Endothelial Cell Adhesion Molecule-1 - metabolism</subject><subject>Risk Factors</subject><subject>submucosal plexus</subject><issn>0309-0167</issn><issn>1365-2559</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kdFuFCEUhonR2LV64QsYEm_0YloYBlguTaPdJk1NbI2XhIWzHdqZYQtM1309n0xmd9sLE4EEyPn-Pxx-hN5TckLLOG19OqG1YOoFmlEmeFVzrl6iGWFEVYQKeYTepHRHCJWsrl-jo1LnUtVshv5cwQb7IfnbNqdyyAHnFnC37ddteDTJjp2JuPc2BjOYHPotDqsdYkMXBmwGt7tFn-6nSg_ZpLJgMsPrG7rjIthsOmzNYCHisMzGD-DwxucWP4xmyD6b7B9hkrfBpYNtBKic76E8LwxF7_ytn3yKXRhSjqPNpfAWvVqZLsG7w36Mfn77enO2qC6_n1-cfbmsbFMLVVkHFhSdO6dg2Si3tA0XzVyBoMwCcZwAMZbXXMJ8pYhwlBAimZSCKC6kYMfo0953HcPDCCnr3icLXWcGCGPSVChZ03kjWUE__oPehTGWFnaUIE2ZpFCf91T53JQirPQ6-t7EraZET8HqEqzeBVvYDwfHcdmDeyafkizA6R7Y-A62_3fSi4vrJ8tqr_Apw-9nhYn3WpS-uf51da6va04XzQ-mGfsLwgfAJA</recordid><startdate>201508</startdate><enddate>201508</enddate><creator>Brown, Peter J</creator><creator>Toh, Eu-Wing</creator><creator>Smith, Katherine J E</creator><creator>Jones, Pamela</creator><creator>Treanor, Darren</creator><creator>Magee, Derek</creator><creator>Burke, Dermot</creator><creator>Quirke, Phil</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201508</creationdate><title>New insights into the lymphovascular microanatomy of the colon and the risk of metastases in pT1 colorectal cancer obtained with quantitative methods and three-dimensional digital reconstruction</title><author>Brown, Peter J ; Toh, Eu-Wing ; Smith, Katherine J E ; Jones, Pamela ; Treanor, Darren ; Magee, Derek ; Burke, Dermot ; Quirke, Phil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4269-cdece918dd9eb49dbc456489e613ce0d50e0ac5257e8f906d1000737760956763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aged</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>CD31</topic><topic>colorectal cancer</topic><topic>Colorectal Neoplasms - blood supply</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Computer Simulation</topic><topic>Early Detection of Cancer - methods</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted</topic><topic>Imaging, Three-Dimensional</topic><topic>Intestinal Mucosa - pathology</topic><topic>Lymph Nodes - pathology</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>microvessel anatomy</topic><topic>Middle Aged</topic><topic>Neoplasm Invasiveness</topic><topic>Neovascularization, Pathologic - metabolism</topic><topic>Neovascularization, Pathologic - pathology</topic><topic>Platelet Endothelial Cell Adhesion Molecule-1 - metabolism</topic><topic>Risk Factors</topic><topic>submucosal plexus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, Peter J</creatorcontrib><creatorcontrib>Toh, Eu-Wing</creatorcontrib><creatorcontrib>Smith, Katherine J E</creatorcontrib><creatorcontrib>Jones, Pamela</creatorcontrib><creatorcontrib>Treanor, Darren</creatorcontrib><creatorcontrib>Magee, Derek</creatorcontrib><creatorcontrib>Burke, Dermot</creatorcontrib><creatorcontrib>Quirke, Phil</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Histopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, Peter J</au><au>Toh, Eu-Wing</au><au>Smith, Katherine J E</au><au>Jones, Pamela</au><au>Treanor, Darren</au><au>Magee, Derek</au><au>Burke, Dermot</au><au>Quirke, Phil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New insights into the lymphovascular microanatomy of the colon and the risk of metastases in pT1 colorectal cancer obtained with quantitative methods and three-dimensional digital reconstruction</atitle><jtitle>Histopathology</jtitle><addtitle>Histopathology</addtitle><date>2015-08</date><risdate>2015</risdate><volume>67</volume><issue>2</issue><spage>167</spage><epage>175</epage><pages>167-175</pages><issn>0309-0167</issn><eissn>1365-2559</eissn><coden>HISTDD</coden><abstract>Aims
UK faecal occult blood test screening has tripled the proportion of pT1 colorectal cancers. The risk of metastasis is predicted by depth of invasion, suggesting that access to deep lymphovascular vessels is important. The aim of this study was to quantify the distribution and size of the submucosal vasculature, and generate a novel three‐dimensional (3D) model to validate the findings.
Methods and results
Thirty samples of normal large bowel wall were immunostained with CD31, a vascular endothelium marker, to identify blood vessels, which were quantified and digitally analysed for their number, circumference, area and diameter in the deep mucosa and submucosa (Sm1, Sm2, and Sm3). The model required serial sections, a double immunostain (using CD31 and D2‐40), and 3D reconstruction. Significant differences were shown between submucosal layers in the number, circumference and area of vessels (P < 0.001). Blood vessels were most numerous in the mucosa (11.79 vessels/0.2 mm2) but smaller [median area of 247 μm2, interquartile range (IQR) 162–373 μm2] than in Sm2, where they were fewer in number (6.92 vessels/0.2 mm2) but considerably larger (2086 μm2, IQR 1007–4784 μm2). The 3D model generated novel observations on lymphovascular structures.
Conclusions
The number and size of blood vessels do not increase with depth of submucosa, as hypothesized. The distribution of vessels suggests that we should investigate the area or volume of submucosal invasion rather than the depth.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>25557923</pmid><doi>10.1111/his.12639</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Biomarkers, Tumor - metabolism CD31 colorectal cancer Colorectal Neoplasms - blood supply Colorectal Neoplasms - pathology Computer Simulation Early Detection of Cancer - methods Endothelium, Vascular - metabolism Female Humans Image Processing, Computer-Assisted Imaging, Three-Dimensional Intestinal Mucosa - pathology Lymph Nodes - pathology Lymphatic Metastasis Male microvessel anatomy Middle Aged Neoplasm Invasiveness Neovascularization, Pathologic - metabolism Neovascularization, Pathologic - pathology Platelet Endothelial Cell Adhesion Molecule-1 - metabolism Risk Factors submucosal plexus |
| title | New insights into the lymphovascular microanatomy of the colon and the risk of metastases in pT1 colorectal cancer obtained with quantitative methods and three-dimensional digital reconstruction |
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