Catalytic antibodies generated via heterologous immunization

Catalytic antibodies generated via heterologous immunization

The mechanism of a selected reaction constitutes a basis for the rational design of haptens which induce catalytic antibodies. Our recent studies on esterolytic antibodies suggested that efficient catalysis could be achieved with the aid of both acidic and basic amino acid residues appropriately dis...

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Veröffentlicht in:Journal of the American Chemical Society 1994-06, Vol.116 (13), p.6025-6026
Hauptverfasser: Suga, Hiroaki, Ersoy, Oguz, Williams, Simon F, Tsumuraya, Takeshi, Margolies, Michael N, Sinskey, Anthony J, Masamune, Satoru
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container_end_page 6026
container_issue 13
container_start_page 6025
container_title Journal of the American Chemical Society
container_volume 116
creator Suga, Hiroaki
Ersoy, Oguz
Williams, Simon F
Tsumuraya, Takeshi
Margolies, Michael N
Sinskey, Anthony J
Masamune, Satoru
description The mechanism of a selected reaction constitutes a basis for the rational design of haptens which induce catalytic antibodies. Our recent studies on esterolytic antibodies suggested that efficient catalysis could be achieved with the aid of both acidic and basic amino acid residues appropriately disposed in the combining site of an antibody, and that these residues would be induced to a zwitterionic hapten. Normally, however, this type of hapten synthesis is not a simple task, and it was thought that a heterologous immunization strategy (vide infra) might serve as an alternative to the use of a zwitterionic hapten for the generation of catalytic antibodies. We describe herein the outcome of this strategy, as compared with that of the standard homologous immunization.
doi_str_mv 10.1021/ja00092a086
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title Catalytic antibodies generated via heterologous immunization
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