Detection of panel-reactive anti-HLA class I antibodies by enzyme-linked immunosorbent assay or lymphocytotoxicity: Results of a blinded, controlled multicenter study

A soluble HLA ELISA for the detection of anti-HLA class I IgG antibodies was developed and compared to complement-dependent microlymphocytotoxicity. ELISA plates were coated with a panel of sHLA class I antigens isolated from the culture supernatants of 46 different EBV-transformed phenotyped B-cell...

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Veröffentlicht in:	Human immunology 1995-09, Vol.44 (1), p.1-11
Hauptverfasser:	Buelow, Roland, Mercier, Isabelle, Glanville, Linda, Regan, Jeffrey, Ellingson, Laura, Janda, Gerald, Claas, Frans, Colombe, Beth, Gelder, Frank, Grosse-Wilde, Hans, Orosz, Charles, Westhoff, Ulrike, Voegeler, Udo, Monteiro, Francisco, Pouletty, Philippe
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibody Specificity Cell Line, Transformed Cytotoxicity Tests, Immunologic Enzyme-Linked Immunosorbent Assay Histocompatibility Testing - methods HLA-A Antigens - immunology HLA-B Antigens - immunology Humans Immunoglobulin G - analysis Immunoglobulin G - immunology Isoantibodies - analysis Isoantibodies - immunology Laboratories - standards Reproducibility of Results Single-Blind Method
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