Salivary Urease and ADS Enzymatic Activity as Endogenous Protection against Dental Caries in Children
The aim of this cross sectional study was to evaluate the ureolytic and arginolytic activities of saliva in children and associate them with their caries status. 65, 8 year old children, were randomly selected. The ureolytic and arginolytic activity of non stimulated saliva was studied and associate...
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| Veröffentlicht in: | The Journal of clinical pediatric dentistry 2015, Vol.39 (4), p.358-363 |
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| container_title | The Journal of clinical pediatric dentistry |
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| creator | Moncada, G Maureira, J Neira, M Reyes, E Oliveira Junior, O B Faleiros, S Palma, P Corsini, G Ugalde, C Gordan, V V Yevenes, I |
| description | The aim of this cross sectional study was to evaluate the ureolytic and arginolytic activities of saliva in children and associate them with their caries status.
65, 8 year old children, were randomly selected. The ureolytic and arginolytic activity of non stimulated saliva was studied and associated with DMFT and dmft index. Saliva of children were sampled under fasting conditions; Children refrained from any oral hygiene procedures during the 12 hours that preceded the sample collection. Caries activity was scored and divided in 3 groups: Group A: Index zero: without lesions; Group B: Moderate Index: 1 to 3 enamel caries lesions; and Group C: High Index: more than 4 dentin caries lesions.
DMFT scores were moderate: 0.4(±0.79) and dmft: 2.78(±2.45). Results expressed in μmol/min/mg/protein, for urease activity were statistically significant (p=0.048): Group A= 0.69 (±0.7); Group B= 0.45 (±0.43); and Group C= 0.39 (±0.55). The arginine deiminase activity was not statistically significant (p=0.16): Group A= 2.53 (±1.42), Group B= 2.31 (±1.57) and Group C= 1.97 (±2.0).
Higher levels of ureolytic (statistically significant) and arginolytic activity (trend) in saliva were associated with lower DMFT/dmft scores in 8 year old children. There was a higher production of ammonia from the arginine deiminase system than the urease enzyme in saliva (p>0.05). |
| doi_str_mv | 10.17796/1053-4628-39.4.358 |
| format | Article |
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65, 8 year old children, were randomly selected. The ureolytic and arginolytic activity of non stimulated saliva was studied and associated with DMFT and dmft index. Saliva of children were sampled under fasting conditions; Children refrained from any oral hygiene procedures during the 12 hours that preceded the sample collection. Caries activity was scored and divided in 3 groups: Group A: Index zero: without lesions; Group B: Moderate Index: 1 to 3 enamel caries lesions; and Group C: High Index: more than 4 dentin caries lesions.
DMFT scores were moderate: 0.4(±0.79) and dmft: 2.78(±2.45). Results expressed in μmol/min/mg/protein, for urease activity were statistically significant (p=0.048): Group A= 0.69 (±0.7); Group B= 0.45 (±0.43); and Group C= 0.39 (±0.55). The arginine deiminase activity was not statistically significant (p=0.16): Group A= 2.53 (±1.42), Group B= 2.31 (±1.57) and Group C= 1.97 (±2.0).
Higher levels of ureolytic (statistically significant) and arginolytic activity (trend) in saliva were associated with lower DMFT/dmft scores in 8 year old children. There was a higher production of ammonia from the arginine deiminase system than the urease enzyme in saliva (p>0.05).</description><identifier>ISSN: 1053-4628</identifier><identifier>EISSN: 1557-5268</identifier><identifier>DOI: 10.17796/1053-4628-39.4.358</identifier><identifier>PMID: 26161608</identifier><language>eng</language><publisher>United States: Journal of Clinical Pediatric Dentistry</publisher><subject>Ammonia - analysis ; Child ; Cross-Sectional Studies ; Dental Caries - enzymology ; Dental Caries - pathology ; Dentistry ; DMF Index ; Fasting - physiology ; Female ; Humans ; Hydrolases - analysis ; Male ; Population Surveillance ; Saliva - enzymology ; Salivary Proteins and Peptides - analysis ; Social Class ; Tooth, Deciduous - pathology ; Urease - analysis</subject><ispartof>The Journal of clinical pediatric dentistry, 2015, Vol.39 (4), p.358-363</ispartof><rights>Copyright Journal of Clinical Pediatric Dentistry Summer 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-bd9d24c3aeb1f5a0da0bd874e8d27e3902dc81dadd47d7f960871c369b958ac43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26161608$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moncada, G</creatorcontrib><creatorcontrib>Maureira, J</creatorcontrib><creatorcontrib>Neira, M</creatorcontrib><creatorcontrib>Reyes, E</creatorcontrib><creatorcontrib>Oliveira Junior, O B</creatorcontrib><creatorcontrib>Faleiros, S</creatorcontrib><creatorcontrib>Palma, P</creatorcontrib><creatorcontrib>Corsini, G</creatorcontrib><creatorcontrib>Ugalde, C</creatorcontrib><creatorcontrib>Gordan, V V</creatorcontrib><creatorcontrib>Yevenes, I</creatorcontrib><title>Salivary Urease and ADS Enzymatic Activity as Endogenous Protection against Dental Caries in Children</title><title>The Journal of clinical pediatric dentistry</title><addtitle>J Clin Pediatr Dent</addtitle><description>The aim of this cross sectional study was to evaluate the ureolytic and arginolytic activities of saliva in children and associate them with their caries status.
65, 8 year old children, were randomly selected. The ureolytic and arginolytic activity of non stimulated saliva was studied and associated with DMFT and dmft index. Saliva of children were sampled under fasting conditions; Children refrained from any oral hygiene procedures during the 12 hours that preceded the sample collection. Caries activity was scored and divided in 3 groups: Group A: Index zero: without lesions; Group B: Moderate Index: 1 to 3 enamel caries lesions; and Group C: High Index: more than 4 dentin caries lesions.
DMFT scores were moderate: 0.4(±0.79) and dmft: 2.78(±2.45). Results expressed in μmol/min/mg/protein, for urease activity were statistically significant (p=0.048): Group A= 0.69 (±0.7); Group B= 0.45 (±0.43); and Group C= 0.39 (±0.55). The arginine deiminase activity was not statistically significant (p=0.16): Group A= 2.53 (±1.42), Group B= 2.31 (±1.57) and Group C= 1.97 (±2.0).
Higher levels of ureolytic (statistically significant) and arginolytic activity (trend) in saliva were associated with lower DMFT/dmft scores in 8 year old children. There was a higher production of ammonia from the arginine deiminase system than the urease enzyme in saliva (p>0.05).</description><subject>Ammonia - analysis</subject><subject>Child</subject><subject>Cross-Sectional Studies</subject><subject>Dental Caries - enzymology</subject><subject>Dental Caries - pathology</subject><subject>Dentistry</subject><subject>DMF Index</subject><subject>Fasting - physiology</subject><subject>Female</subject><subject>Humans</subject><subject>Hydrolases - analysis</subject><subject>Male</subject><subject>Population Surveillance</subject><subject>Saliva - enzymology</subject><subject>Salivary Proteins and Peptides - analysis</subject><subject>Social Class</subject><subject>Tooth, Deciduous - pathology</subject><subject>Urease - analysis</subject><issn>1053-4628</issn><issn>1557-5268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkU1rHDEMhk1oyfcvKBRDL73M1h57_HFcNtskEGghzdlobO3WYdaT2rOB7a-Pt5vkUHSQkB4J6RUhnzibca2t-sZZJxqpWtMIO5Mz0Zkjcsq7Tjddq8yHGr8BJ-SslEfGmFGGHZOTVvFqzJwSvIchPkPe0YeMUJBCCnR-dU-X6e9uA1P0dO6n-BynHYVSs2FcYxq3hf7M44S1NCYKa4ipTPQK0wQDXUCOWGhMdPE7DiFjuiAfVzAUvHz15-Th-_LX4qa5-3F9u5jfNV5oMzV9sKGVXgD2fNUBC8D6YLREE1qNwrI2eMMDhCB10CtbL9DcC2V72xnwUpyTr4e5T3n8s8UyuU0sHocBEtadHVdWtYxJriv65T_0cdzmVLdzXFumuOSyq5Q4UD6PpWRcuaccN1Uux5n79wW3F9ntRXbCOunqF2rX59fZ236D4b3nTXbxAhLugn8</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Moncada, G</creator><creator>Maureira, J</creator><creator>Neira, M</creator><creator>Reyes, E</creator><creator>Oliveira Junior, O B</creator><creator>Faleiros, S</creator><creator>Palma, P</creator><creator>Corsini, G</creator><creator>Ugalde, C</creator><creator>Gordan, V V</creator><creator>Yevenes, I</creator><general>Journal of Clinical Pediatric Dentistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>2015</creationdate><title>Salivary Urease and ADS Enzymatic Activity as Endogenous Protection against Dental Caries in Children</title><author>Moncada, G ; Maureira, J ; Neira, M ; Reyes, E ; Oliveira Junior, O B ; Faleiros, S ; Palma, P ; Corsini, G ; Ugalde, C ; Gordan, V V ; Yevenes, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-bd9d24c3aeb1f5a0da0bd874e8d27e3902dc81dadd47d7f960871c369b958ac43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Ammonia - analysis</topic><topic>Child</topic><topic>Cross-Sectional Studies</topic><topic>Dental Caries - enzymology</topic><topic>Dental Caries - pathology</topic><topic>Dentistry</topic><topic>DMF Index</topic><topic>Fasting - physiology</topic><topic>Female</topic><topic>Humans</topic><topic>Hydrolases - analysis</topic><topic>Male</topic><topic>Population Surveillance</topic><topic>Saliva - enzymology</topic><topic>Salivary Proteins and Peptides - analysis</topic><topic>Social Class</topic><topic>Tooth, Deciduous - pathology</topic><topic>Urease - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moncada, G</creatorcontrib><creatorcontrib>Maureira, J</creatorcontrib><creatorcontrib>Neira, M</creatorcontrib><creatorcontrib>Reyes, E</creatorcontrib><creatorcontrib>Oliveira Junior, O B</creatorcontrib><creatorcontrib>Faleiros, S</creatorcontrib><creatorcontrib>Palma, P</creatorcontrib><creatorcontrib>Corsini, G</creatorcontrib><creatorcontrib>Ugalde, C</creatorcontrib><creatorcontrib>Gordan, V V</creatorcontrib><creatorcontrib>Yevenes, I</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of clinical pediatric dentistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moncada, G</au><au>Maureira, J</au><au>Neira, M</au><au>Reyes, E</au><au>Oliveira Junior, O B</au><au>Faleiros, S</au><au>Palma, P</au><au>Corsini, G</au><au>Ugalde, C</au><au>Gordan, V V</au><au>Yevenes, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Salivary Urease and ADS Enzymatic Activity as Endogenous Protection against Dental Caries in Children</atitle><jtitle>The Journal of clinical pediatric dentistry</jtitle><addtitle>J Clin Pediatr Dent</addtitle><date>2015</date><risdate>2015</risdate><volume>39</volume><issue>4</issue><spage>358</spage><epage>363</epage><pages>358-363</pages><issn>1053-4628</issn><eissn>1557-5268</eissn><abstract>The aim of this cross sectional study was to evaluate the ureolytic and arginolytic activities of saliva in children and associate them with their caries status.
65, 8 year old children, were randomly selected. The ureolytic and arginolytic activity of non stimulated saliva was studied and associated with DMFT and dmft index. Saliva of children were sampled under fasting conditions; Children refrained from any oral hygiene procedures during the 12 hours that preceded the sample collection. Caries activity was scored and divided in 3 groups: Group A: Index zero: without lesions; Group B: Moderate Index: 1 to 3 enamel caries lesions; and Group C: High Index: more than 4 dentin caries lesions.
DMFT scores were moderate: 0.4(±0.79) and dmft: 2.78(±2.45). Results expressed in μmol/min/mg/protein, for urease activity were statistically significant (p=0.048): Group A= 0.69 (±0.7); Group B= 0.45 (±0.43); and Group C= 0.39 (±0.55). The arginine deiminase activity was not statistically significant (p=0.16): Group A= 2.53 (±1.42), Group B= 2.31 (±1.57) and Group C= 1.97 (±2.0).
Higher levels of ureolytic (statistically significant) and arginolytic activity (trend) in saliva were associated with lower DMFT/dmft scores in 8 year old children. There was a higher production of ammonia from the arginine deiminase system than the urease enzyme in saliva (p>0.05).</abstract><cop>United States</cop><pub>Journal of Clinical Pediatric Dentistry</pub><pmid>26161608</pmid><doi>10.17796/1053-4628-39.4.358</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Ammonia - analysis Child Cross-Sectional Studies Dental Caries - enzymology Dental Caries - pathology Dentistry DMF Index Fasting - physiology Female Humans Hydrolases - analysis Male Population Surveillance Saliva - enzymology Salivary Proteins and Peptides - analysis Social Class Tooth, Deciduous - pathology Urease - analysis |
| title | Salivary Urease and ADS Enzymatic Activity as Endogenous Protection against Dental Caries in Children |
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