Human pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor-1 alpha. Characterization and kinetic analysis of wild-type and mutant enzymes

Pterin-4a-carbinolamine dehydratase/dimerization cofactor for hepatocyte nuclear factor-1 alpha is a protein with two different functions. We have overexpressed and purified the human wild-type protein, and its Cys81Ser and Cys81Arg mutants. The Cys81Arg mutant has been proposed to be causative in a...

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Veröffentlicht in:	European journal of biochemistry 1995-07, Vol.231 (2), p.414-423
Hauptverfasser:	Köster, S, Thöny, B, Macheroux, P, Curtius, H C, Heizmann, C W, Pfleiderer, W, Ghisla, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biopterins - analogs & derivatives Biopterins - pharmacology Catalysis Cysteine - chemistry Cysteine - metabolism Humans Hydro-Lyases - chemistry Hydro-Lyases - genetics Hydro-Lyases - isolation & purification Hydro-Lyases - metabolism Hydrogen-Ion Concentration Hydroxylation Kinetics Liver - enzymology Molecular Sequence Data Mutagenesis Phenylalanine - metabolism Phenylalanine Hydroxylase - metabolism Protein Binding Pterins - metabolism Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Spectrometry, Fluorescence Temperature Transcription Factors - chemistry Transcription Factors - genetics Transcription Factors - isolation & purification Transcription Factors - metabolism Ultraviolet Rays
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