Systemic inflammation and oxidative stress in hemodialysis patients are associated with down-regulation of Nrf2
Background Oxidative stress and inflammation are common features and the main mediators of progression of chronic kidney disease (CKD) and its cardiovascular complications. Under normal conditions, oxidative stress activates the transcription factor, nuclear factor E2-related factor 2 (Nrf2), which...
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| Veröffentlicht in: | Journal of nephrology 2015-08, Vol.28 (4), p.495-501 |
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| creator | Pedruzzi, Liliana M. Cardozo, Ludmila F. M. F. Daleprane, Julio B. Stockler-Pinto, Milena B. Monteiro, Elisa B. Leite, Maurilo Vaziri, Nosratola D. Mafra, Denise |
| description | Background
Oxidative stress and inflammation are common features and the main mediators of progression of chronic kidney disease (CKD) and its cardiovascular complications. Under normal conditions, oxidative stress activates the transcription factor, nuclear factor E2-related factor 2 (Nrf2), which is the master regulator of genes encoding antioxidant and detoxifying enzymes and related proteins. The available data on expression of Nrf2 and its key target gene products in CKD patients is limited. We therefore investigated this topic in a group of CKD patients on hemodialysis.
Methods
Twenty adult hemodialysis (HD) patients (aged 54.9 ± 15.2 years) and 11 healthy individuals (aged 50.9 ± 8.0 years) were enrolled. Peripheral blood mononuclear cells (PBMC) were isolated and processed for expression of nuclear factor-κB (NF-κB), Nrf2, heme oxygenase-1 and NADPH: quinoneoxidoreductase 1 (NQO1) by quantitative real-time polymerase chain reaction and western blot analysis. Plasma malondialdehyde (MDA) and tumor necrosis factor-alpha (TNF-α) levels were measured.
Results
Peripheral blood mononuclear cells from HD patients had significantly lower NQO1 and Nrf2 mRNA expressions (0.58 ± 0.35 vs. 1.13 ± 0.64, p = 0.005), and significantly higher NF-κB expression (2.18 ± 0.8 vs. 1.04 ± 0.22, p = 0.0001) compared to the healthy individuals. The NF-κB expression was inversely correlated with Nrf2 levels (r = −0.54, p |
| doi_str_mv | 10.1007/s40620-014-0162-0 |
| format | Article |
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Oxidative stress and inflammation are common features and the main mediators of progression of chronic kidney disease (CKD) and its cardiovascular complications. Under normal conditions, oxidative stress activates the transcription factor, nuclear factor E2-related factor 2 (Nrf2), which is the master regulator of genes encoding antioxidant and detoxifying enzymes and related proteins. The available data on expression of Nrf2 and its key target gene products in CKD patients is limited. We therefore investigated this topic in a group of CKD patients on hemodialysis.
Methods
Twenty adult hemodialysis (HD) patients (aged 54.9 ± 15.2 years) and 11 healthy individuals (aged 50.9 ± 8.0 years) were enrolled. Peripheral blood mononuclear cells (PBMC) were isolated and processed for expression of nuclear factor-κB (NF-κB), Nrf2, heme oxygenase-1 and NADPH: quinoneoxidoreductase 1 (NQO1) by quantitative real-time polymerase chain reaction and western blot analysis. Plasma malondialdehyde (MDA) and tumor necrosis factor-alpha (TNF-α) levels were measured.
Results
Peripheral blood mononuclear cells from HD patients had significantly lower NQO1 and Nrf2 mRNA expressions (0.58 ± 0.35 vs. 1.13 ± 0.64, p = 0.005), and significantly higher NF-κB expression (2.18 ± 0.8 vs. 1.04 ± 0.22, p = 0.0001) compared to the healthy individuals. The NF-κB expression was inversely correlated with Nrf2 levels (r = −0.54, p < 0.01) in CKD patients. Plasma MDA and TNF-α levels were significantly higher in CKD patients than in the healthy individuals.
Conclusions
Up-regulation of NFκB in the CKD patients’ PBMC is coupled to down-regulation of Nrf2 and NQO1 expression. These observations are consistent with recent findings in CKD animals and point to the contribution of the impaired Nrf2 system in the pathogenesis of oxidative stress and inflammation in hemodialysis patients.</description><identifier>ISSN: 1121-8428</identifier><identifier>EISSN: 1724-6059</identifier><identifier>DOI: 10.1007/s40620-014-0162-0</identifier><identifier>PMID: 25585822</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Adult ; Aged ; Biomarkers - blood ; Case-Control Studies ; Down-Regulation ; Female ; Humans ; Inflammation - blood ; Inflammation - diagnosis ; Inflammation - etiology ; Inflammation - genetics ; Inflammation - immunology ; Inflammation Mediators - blood ; Leukocytes, Mononuclear - immunology ; Leukocytes, Mononuclear - metabolism ; Male ; Malondialdehyde - blood ; Medicine ; Medicine & Public Health ; Middle Aged ; NAD(P)H Dehydrogenase (Quinone) - genetics ; NAD(P)H Dehydrogenase (Quinone) - metabolism ; Nephrology ; NF-E2-Related Factor 2 - genetics ; NF-E2-Related Factor 2 - metabolism ; NF-kappa B - metabolism ; Original Article ; Oxidative Stress ; Renal Dialysis - adverse effects ; Renal Insufficiency, Chronic - blood ; Renal Insufficiency, Chronic - diagnosis ; Renal Insufficiency, Chronic - immunology ; Renal Insufficiency, Chronic - therapy ; RNA, Messenger - metabolism ; Tumor Necrosis Factor-alpha - blood ; Urology</subject><ispartof>Journal of nephrology, 2015-08, Vol.28 (4), p.495-501</ispartof><rights>Italian Society of Nephrology 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-eef274aa226c178b628ffa2c419ea43abd624f8b247e6b9a3709f54fac9ba4133</citedby><cites>FETCH-LOGICAL-c387t-eef274aa226c178b628ffa2c419ea43abd624f8b247e6b9a3709f54fac9ba4133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40620-014-0162-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40620-014-0162-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25585822$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pedruzzi, Liliana M.</creatorcontrib><creatorcontrib>Cardozo, Ludmila F. M. F.</creatorcontrib><creatorcontrib>Daleprane, Julio B.</creatorcontrib><creatorcontrib>Stockler-Pinto, Milena B.</creatorcontrib><creatorcontrib>Monteiro, Elisa B.</creatorcontrib><creatorcontrib>Leite, Maurilo</creatorcontrib><creatorcontrib>Vaziri, Nosratola D.</creatorcontrib><creatorcontrib>Mafra, Denise</creatorcontrib><title>Systemic inflammation and oxidative stress in hemodialysis patients are associated with down-regulation of Nrf2</title><title>Journal of nephrology</title><addtitle>J Nephrol</addtitle><addtitle>J Nephrol</addtitle><description>Background
Oxidative stress and inflammation are common features and the main mediators of progression of chronic kidney disease (CKD) and its cardiovascular complications. Under normal conditions, oxidative stress activates the transcription factor, nuclear factor E2-related factor 2 (Nrf2), which is the master regulator of genes encoding antioxidant and detoxifying enzymes and related proteins. The available data on expression of Nrf2 and its key target gene products in CKD patients is limited. We therefore investigated this topic in a group of CKD patients on hemodialysis.
Methods
Twenty adult hemodialysis (HD) patients (aged 54.9 ± 15.2 years) and 11 healthy individuals (aged 50.9 ± 8.0 years) were enrolled. Peripheral blood mononuclear cells (PBMC) were isolated and processed for expression of nuclear factor-κB (NF-κB), Nrf2, heme oxygenase-1 and NADPH: quinoneoxidoreductase 1 (NQO1) by quantitative real-time polymerase chain reaction and western blot analysis. Plasma malondialdehyde (MDA) and tumor necrosis factor-alpha (TNF-α) levels were measured.
Results
Peripheral blood mononuclear cells from HD patients had significantly lower NQO1 and Nrf2 mRNA expressions (0.58 ± 0.35 vs. 1.13 ± 0.64, p = 0.005), and significantly higher NF-κB expression (2.18 ± 0.8 vs. 1.04 ± 0.22, p = 0.0001) compared to the healthy individuals. The NF-κB expression was inversely correlated with Nrf2 levels (r = −0.54, p < 0.01) in CKD patients. Plasma MDA and TNF-α levels were significantly higher in CKD patients than in the healthy individuals.
Conclusions
Up-regulation of NFκB in the CKD patients’ PBMC is coupled to down-regulation of Nrf2 and NQO1 expression. These observations are consistent with recent findings in CKD animals and point to the contribution of the impaired Nrf2 system in the pathogenesis of oxidative stress and inflammation in hemodialysis patients.</description><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers - blood</subject><subject>Case-Control Studies</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Humans</subject><subject>Inflammation - blood</subject><subject>Inflammation - diagnosis</subject><subject>Inflammation - etiology</subject><subject>Inflammation - genetics</subject><subject>Inflammation - immunology</subject><subject>Inflammation Mediators - blood</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Male</subject><subject>Malondialdehyde - blood</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>NAD(P)H Dehydrogenase (Quinone) - genetics</subject><subject>NAD(P)H Dehydrogenase (Quinone) - metabolism</subject><subject>Nephrology</subject><subject>NF-E2-Related Factor 2 - genetics</subject><subject>NF-E2-Related Factor 2 - metabolism</subject><subject>NF-kappa B - metabolism</subject><subject>Original Article</subject><subject>Oxidative Stress</subject><subject>Renal Dialysis - adverse effects</subject><subject>Renal Insufficiency, Chronic - blood</subject><subject>Renal Insufficiency, Chronic - diagnosis</subject><subject>Renal Insufficiency, Chronic - immunology</subject><subject>Renal Insufficiency, Chronic - therapy</subject><subject>RNA, Messenger - metabolism</subject><subject>Tumor Necrosis Factor-alpha - blood</subject><subject>Urology</subject><issn>1121-8428</issn><issn>1724-6059</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtv3TAMRoUiRfNof0CXQmMXJxItPzQWQR8BgnRoOwu0TSUKbOtWtJPef19dOMmYgSAFHn6AjhAftTrXSjUXbFQNqlDa5KqhUG_EiW7AFLWq7FGeNeiiNdAei1Pme6WgqsC8E8e5t1ULcCLirz0vNIVehtmPOE24hDhLnAcZ_4Uhvx5I8pKIORPyjqY4BBz3HFju8pbmhSUmksgc-4ALDfIxLHdyiI9zkeh2HbfE6OVN8vBevPU4Mn146mfiz7evvy9_FNc_v19dfrku-rJtloLIQ2MQAepeN21XQ-s9Qm-0JTQldkMNxrcdmIbqzmLZKOsr47G3HRpdlmfi85a7S_HvSry4KXBP44gzxZWdrm3VVNbaA6o3tE-ROZF3uxQmTHunlTt4dptnlz27g2en8s2np_i1m2h4uXgWmwHYAM6r-ZaSu49rmvOXX0n9D0ehiro</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Pedruzzi, Liliana M.</creator><creator>Cardozo, Ludmila F. M. F.</creator><creator>Daleprane, Julio B.</creator><creator>Stockler-Pinto, Milena B.</creator><creator>Monteiro, Elisa B.</creator><creator>Leite, Maurilo</creator><creator>Vaziri, Nosratola D.</creator><creator>Mafra, Denise</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150801</creationdate><title>Systemic inflammation and oxidative stress in hemodialysis patients are associated with down-regulation of Nrf2</title><author>Pedruzzi, Liliana M. ; Cardozo, Ludmila F. M. F. ; Daleprane, Julio B. ; Stockler-Pinto, Milena B. ; Monteiro, Elisa B. ; Leite, Maurilo ; Vaziri, Nosratola D. ; Mafra, Denise</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-eef274aa226c178b628ffa2c419ea43abd624f8b247e6b9a3709f54fac9ba4133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers - blood</topic><topic>Case-Control Studies</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Humans</topic><topic>Inflammation - blood</topic><topic>Inflammation - diagnosis</topic><topic>Inflammation - etiology</topic><topic>Inflammation - genetics</topic><topic>Inflammation - immunology</topic><topic>Inflammation Mediators - blood</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Male</topic><topic>Malondialdehyde - blood</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>NAD(P)H Dehydrogenase (Quinone) - genetics</topic><topic>NAD(P)H Dehydrogenase (Quinone) - metabolism</topic><topic>Nephrology</topic><topic>NF-E2-Related Factor 2 - genetics</topic><topic>NF-E2-Related Factor 2 - metabolism</topic><topic>NF-kappa B - metabolism</topic><topic>Original Article</topic><topic>Oxidative Stress</topic><topic>Renal Dialysis - adverse effects</topic><topic>Renal Insufficiency, Chronic - blood</topic><topic>Renal Insufficiency, Chronic - diagnosis</topic><topic>Renal Insufficiency, Chronic - immunology</topic><topic>Renal Insufficiency, Chronic - therapy</topic><topic>RNA, Messenger - metabolism</topic><topic>Tumor Necrosis Factor-alpha - blood</topic><topic>Urology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pedruzzi, Liliana M.</creatorcontrib><creatorcontrib>Cardozo, Ludmila F. M. F.</creatorcontrib><creatorcontrib>Daleprane, Julio B.</creatorcontrib><creatorcontrib>Stockler-Pinto, Milena B.</creatorcontrib><creatorcontrib>Monteiro, Elisa B.</creatorcontrib><creatorcontrib>Leite, Maurilo</creatorcontrib><creatorcontrib>Vaziri, Nosratola D.</creatorcontrib><creatorcontrib>Mafra, Denise</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of nephrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pedruzzi, Liliana M.</au><au>Cardozo, Ludmila F. M. F.</au><au>Daleprane, Julio B.</au><au>Stockler-Pinto, Milena B.</au><au>Monteiro, Elisa B.</au><au>Leite, Maurilo</au><au>Vaziri, Nosratola D.</au><au>Mafra, Denise</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Systemic inflammation and oxidative stress in hemodialysis patients are associated with down-regulation of Nrf2</atitle><jtitle>Journal of nephrology</jtitle><stitle>J Nephrol</stitle><addtitle>J Nephrol</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>28</volume><issue>4</issue><spage>495</spage><epage>501</epage><pages>495-501</pages><issn>1121-8428</issn><eissn>1724-6059</eissn><abstract>Background
Oxidative stress and inflammation are common features and the main mediators of progression of chronic kidney disease (CKD) and its cardiovascular complications. Under normal conditions, oxidative stress activates the transcription factor, nuclear factor E2-related factor 2 (Nrf2), which is the master regulator of genes encoding antioxidant and detoxifying enzymes and related proteins. The available data on expression of Nrf2 and its key target gene products in CKD patients is limited. We therefore investigated this topic in a group of CKD patients on hemodialysis.
Methods
Twenty adult hemodialysis (HD) patients (aged 54.9 ± 15.2 years) and 11 healthy individuals (aged 50.9 ± 8.0 years) were enrolled. Peripheral blood mononuclear cells (PBMC) were isolated and processed for expression of nuclear factor-κB (NF-κB), Nrf2, heme oxygenase-1 and NADPH: quinoneoxidoreductase 1 (NQO1) by quantitative real-time polymerase chain reaction and western blot analysis. Plasma malondialdehyde (MDA) and tumor necrosis factor-alpha (TNF-α) levels were measured.
Results
Peripheral blood mononuclear cells from HD patients had significantly lower NQO1 and Nrf2 mRNA expressions (0.58 ± 0.35 vs. 1.13 ± 0.64, p = 0.005), and significantly higher NF-κB expression (2.18 ± 0.8 vs. 1.04 ± 0.22, p = 0.0001) compared to the healthy individuals. The NF-κB expression was inversely correlated with Nrf2 levels (r = −0.54, p < 0.01) in CKD patients. Plasma MDA and TNF-α levels were significantly higher in CKD patients than in the healthy individuals.
Conclusions
Up-regulation of NFκB in the CKD patients’ PBMC is coupled to down-regulation of Nrf2 and NQO1 expression. These observations are consistent with recent findings in CKD animals and point to the contribution of the impaired Nrf2 system in the pathogenesis of oxidative stress and inflammation in hemodialysis patients.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>25585822</pmid><doi>10.1007/s40620-014-0162-0</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Biomarkers - blood Case-Control Studies Down-Regulation Female Humans Inflammation - blood Inflammation - diagnosis Inflammation - etiology Inflammation - genetics Inflammation - immunology Inflammation Mediators - blood Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - metabolism Male Malondialdehyde - blood Medicine Medicine & Public Health Middle Aged NAD(P)H Dehydrogenase (Quinone) - genetics NAD(P)H Dehydrogenase (Quinone) - metabolism Nephrology NF-E2-Related Factor 2 - genetics NF-E2-Related Factor 2 - metabolism NF-kappa B - metabolism Original Article Oxidative Stress Renal Dialysis - adverse effects Renal Insufficiency, Chronic - blood Renal Insufficiency, Chronic - diagnosis Renal Insufficiency, Chronic - immunology Renal Insufficiency, Chronic - therapy RNA, Messenger - metabolism Tumor Necrosis Factor-alpha - blood Urology |
| title | Systemic inflammation and oxidative stress in hemodialysis patients are associated with down-regulation of Nrf2 |
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