Okadaic acid-dependent induction of the urokinase-type plasminogen activator gene associated with stabilization and autoregulation of c-Jun
We have previously shown that the tumor promoter okadaic acid (OA), an inhibitor of protein phosphatases 1 and 2A, transcriptionally induces the urokinase-type plasminogen activator (uPA) gene in LLC-PK1 cells. This induction occurs independently of the protein kinase C- and cAMP-dependent signaling...
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| Veröffentlicht in: | The Journal of biological chemistry 1994-01, Vol.269 (4), p.2887-2894 |
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| creator | LEE, J. S FAVRE, B HEMMINGS, B. A KIEFER, B NAGAMINE, Y |
| description | We have previously shown that the tumor promoter okadaic acid (OA), an inhibitor of protein phosphatases 1 and 2A, transcriptionally
induces the urokinase-type plasminogen activator (uPA) gene in LLC-PK1 cells. This induction occurs independently of the protein
kinase C- and cAMP-dependent signaling pathways. Here we show that a sequence located 2.0 kilobases upstream of the uPA gene,
which resembles an AP-1-recognition sequence, mediates the action of OA. DNA-protein interaction studies, together with mRNA
and protein analyses, indicate that c-Jun, but not c-Fos, is involved in OA-dependent uPA gene induction. The appearance of
high levels of uPA mRNA and DNA binding activity of c-Jun to the AP-1-like site correspond to the appearance of c-Jun accumulation,
suggesting that c-Jun accumulation is a critical event in OA-dependent uPA gene induction. c-Jun protein levels increase significantly
between 100 and 160 min following OA treatment, whereas c-Jun translation increases only slightly in this time frame, suggesting
that post-translation mechanisms are also involved in c-Jun accumulation. Pulse-chase analyses shows that OA specifically
stabilizes c-Jun. We discuss our results with respect to the possibility that protein phosphatase 2A maintains c-Jun in its
down-regulated state in LLC-PK1 cells. |
| doi_str_mv | 10.1016/S0021-9258(17)42025-4 |
| format | Article |
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induces the urokinase-type plasminogen activator (uPA) gene in LLC-PK1 cells. This induction occurs independently of the protein
kinase C- and cAMP-dependent signaling pathways. Here we show that a sequence located 2.0 kilobases upstream of the uPA gene,
which resembles an AP-1-recognition sequence, mediates the action of OA. DNA-protein interaction studies, together with mRNA
and protein analyses, indicate that c-Jun, but not c-Fos, is involved in OA-dependent uPA gene induction. The appearance of
high levels of uPA mRNA and DNA binding activity of c-Jun to the AP-1-like site correspond to the appearance of c-Jun accumulation,
suggesting that c-Jun accumulation is a critical event in OA-dependent uPA gene induction. c-Jun protein levels increase significantly
between 100 and 160 min following OA treatment, whereas c-Jun translation increases only slightly in this time frame, suggesting
that post-translation mechanisms are also involved in c-Jun accumulation. Pulse-chase analyses shows that OA specifically
stabilizes c-Jun. We discuss our results with respect to the possibility that protein phosphatase 2A maintains c-Jun in its
down-regulated state in LLC-PK1 cells.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)42025-4</identifier><identifier>PMID: 8300623</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>8-Bromo Cyclic Adenosine Monophosphate - pharmacology ; Animals ; Base Sequence ; Binding Sites ; Biological and medical sciences ; Blotting, Southern ; Blotting, Western ; Cell Line ; Chloramphenicol O-Acetyltransferase - biosynthesis ; Chloramphenicol O-Acetyltransferase - metabolism ; Colchicine - pharmacology ; Cycloheximide - pharmacology ; DNA Mutational Analysis ; DNA-Binding Proteins - metabolism ; Enzyme Induction ; Ethers, Cyclic - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Enzymologic - drug effects ; Genes, Regulator ; Homeostasis ; Kinetics ; Marine Toxins ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nuclear Proteins - isolation & purification ; Nuclear Proteins - metabolism ; Okadaic Acid ; Oligodeoxyribonucleotides - chemistry ; Oligodeoxyribonucleotides - metabolism ; Oxazoles - pharmacology ; Phosphorylation ; Protein Phosphatase 2 ; Protein Tyrosine Phosphatases - antagonists & inhibitors ; Proto-Oncogene Proteins c-jun - biosynthesis ; Proto-Oncogene Proteins c-jun - metabolism ; Time Factors ; Transcription. Transcription factor. Splicing. Rna processing ; Transcriptional Activation ; Transfection ; Urokinase-Type Plasminogen Activator - biosynthesis ; Urokinase-Type Plasminogen Activator - genetics</subject><ispartof>The Journal of biological chemistry, 1994-01, Vol.269 (4), p.2887-2894</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-9cdf750b7a2e7e93597492d1d08e2f556fb75d07e4eaae4c69225efab6021b613</citedby><cites>FETCH-LOGICAL-c438t-9cdf750b7a2e7e93597492d1d08e2f556fb75d07e4eaae4c69225efab6021b613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4008127$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8300623$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LEE, J. S</creatorcontrib><creatorcontrib>FAVRE, B</creatorcontrib><creatorcontrib>HEMMINGS, B. A</creatorcontrib><creatorcontrib>KIEFER, B</creatorcontrib><creatorcontrib>NAGAMINE, Y</creatorcontrib><title>Okadaic acid-dependent induction of the urokinase-type plasminogen activator gene associated with stabilization and autoregulation of c-Jun</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>We have previously shown that the tumor promoter okadaic acid (OA), an inhibitor of protein phosphatases 1 and 2A, transcriptionally
induces the urokinase-type plasminogen activator (uPA) gene in LLC-PK1 cells. This induction occurs independently of the protein
kinase C- and cAMP-dependent signaling pathways. Here we show that a sequence located 2.0 kilobases upstream of the uPA gene,
which resembles an AP-1-recognition sequence, mediates the action of OA. DNA-protein interaction studies, together with mRNA
and protein analyses, indicate that c-Jun, but not c-Fos, is involved in OA-dependent uPA gene induction. The appearance of
high levels of uPA mRNA and DNA binding activity of c-Jun to the AP-1-like site correspond to the appearance of c-Jun accumulation,
suggesting that c-Jun accumulation is a critical event in OA-dependent uPA gene induction. c-Jun protein levels increase significantly
between 100 and 160 min following OA treatment, whereas c-Jun translation increases only slightly in this time frame, suggesting
that post-translation mechanisms are also involved in c-Jun accumulation. Pulse-chase analyses shows that OA specifically
stabilizes c-Jun. We discuss our results with respect to the possibility that protein phosphatase 2A maintains c-Jun in its
down-regulated state in LLC-PK1 cells.</description><subject>8-Bromo Cyclic Adenosine Monophosphate - pharmacology</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>Chloramphenicol O-Acetyltransferase - biosynthesis</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>Colchicine - pharmacology</subject><subject>Cycloheximide - pharmacology</subject><subject>DNA Mutational Analysis</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Enzyme Induction</subject><subject>Ethers, Cyclic - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Genes, Regulator</subject><subject>Homeostasis</subject><subject>Kinetics</subject><subject>Marine Toxins</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins - isolation & purification</subject><subject>Nuclear Proteins - metabolism</subject><subject>Okadaic Acid</subject><subject>Oligodeoxyribonucleotides - chemistry</subject><subject>Oligodeoxyribonucleotides - metabolism</subject><subject>Oxazoles - pharmacology</subject><subject>Phosphorylation</subject><subject>Protein Phosphatase 2</subject><subject>Protein Tyrosine Phosphatases - antagonists & inhibitors</subject><subject>Proto-Oncogene Proteins c-jun - biosynthesis</subject><subject>Proto-Oncogene Proteins c-jun - metabolism</subject><subject>Time Factors</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Transcriptional Activation</subject><subject>Transfection</subject><subject>Urokinase-Type Plasminogen Activator - biosynthesis</subject><subject>Urokinase-Type Plasminogen Activator - genetics</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kd1u1DAQhS0EKkvhESpZCCG4CNiOHceXqOJXlXoBSNxZE3uyMU3sJXaoyivw0qS7y87NaDTfmZHOIeSCszec8ebtV8YEr4xQ7SuuX0vBhKrkA7LhrK2rWvEfD8nmhDwmT3L-ydaShp-Rs7ZmrBH1hvy9vgEPwVFwwVcedxg9xkJD9IsrIUWaeloGpMucbkKEjFW52yHdjZCnENMW4yot4TeUNNN1Qgo5JxegoKe3oQw0F-jCGP7A_hxET2FZYdwuI_z_4KovS3xKHvUwZnx27Ofk-4f33y4_VVfXHz9fvruqnKzbUhnne61Yp0GgRlMro6URnnvWouiVavpOK880SgRA6RojhMIeumZ1o2t4fU5eHu7u5vRrwVzsFLLDcYSIacmWN0bVrTIrqA6gm1POM_Z2N4cJ5jvLmb0Pwe5DsPcOW67tPgQrV93F8cHSTehPqqPr6_7FcQ_ZwdjPEF3IJ0wy1nKhV-z5ARvCdrgNM9ouJDfgZEVjrLSibXX9D_XonfA</recordid><startdate>19940128</startdate><enddate>19940128</enddate><creator>LEE, J. S</creator><creator>FAVRE, B</creator><creator>HEMMINGS, B. A</creator><creator>KIEFER, B</creator><creator>NAGAMINE, Y</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>19940128</creationdate><title>Okadaic acid-dependent induction of the urokinase-type plasminogen activator gene associated with stabilization and autoregulation of c-Jun</title><author>LEE, J. S ; FAVRE, B ; HEMMINGS, B. A ; KIEFER, B ; NAGAMINE, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-9cdf750b7a2e7e93597492d1d08e2f556fb75d07e4eaae4c69225efab6021b613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>8-Bromo Cyclic Adenosine Monophosphate - pharmacology</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>Chloramphenicol O-Acetyltransferase - biosynthesis</topic><topic>Chloramphenicol O-Acetyltransferase - metabolism</topic><topic>Colchicine - pharmacology</topic><topic>Cycloheximide - pharmacology</topic><topic>DNA Mutational Analysis</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Enzyme Induction</topic><topic>Ethers, Cyclic - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Genes, Regulator</topic><topic>Homeostasis</topic><topic>Kinetics</topic><topic>Marine Toxins</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Proteins - isolation & purification</topic><topic>Nuclear Proteins - metabolism</topic><topic>Okadaic Acid</topic><topic>Oligodeoxyribonucleotides - chemistry</topic><topic>Oligodeoxyribonucleotides - metabolism</topic><topic>Oxazoles - pharmacology</topic><topic>Phosphorylation</topic><topic>Protein Phosphatase 2</topic><topic>Protein Tyrosine Phosphatases - antagonists & inhibitors</topic><topic>Proto-Oncogene Proteins c-jun - biosynthesis</topic><topic>Proto-Oncogene Proteins c-jun - metabolism</topic><topic>Time Factors</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Transcriptional Activation</topic><topic>Transfection</topic><topic>Urokinase-Type Plasminogen Activator - biosynthesis</topic><topic>Urokinase-Type Plasminogen Activator - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LEE, J. S</creatorcontrib><creatorcontrib>FAVRE, B</creatorcontrib><creatorcontrib>HEMMINGS, B. A</creatorcontrib><creatorcontrib>KIEFER, B</creatorcontrib><creatorcontrib>NAGAMINE, Y</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LEE, J. S</au><au>FAVRE, B</au><au>HEMMINGS, B. A</au><au>KIEFER, B</au><au>NAGAMINE, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Okadaic acid-dependent induction of the urokinase-type plasminogen activator gene associated with stabilization and autoregulation of c-Jun</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-01-28</date><risdate>1994</risdate><volume>269</volume><issue>4</issue><spage>2887</spage><epage>2894</epage><pages>2887-2894</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>We have previously shown that the tumor promoter okadaic acid (OA), an inhibitor of protein phosphatases 1 and 2A, transcriptionally
induces the urokinase-type plasminogen activator (uPA) gene in LLC-PK1 cells. This induction occurs independently of the protein
kinase C- and cAMP-dependent signaling pathways. Here we show that a sequence located 2.0 kilobases upstream of the uPA gene,
which resembles an AP-1-recognition sequence, mediates the action of OA. DNA-protein interaction studies, together with mRNA
and protein analyses, indicate that c-Jun, but not c-Fos, is involved in OA-dependent uPA gene induction. The appearance of
high levels of uPA mRNA and DNA binding activity of c-Jun to the AP-1-like site correspond to the appearance of c-Jun accumulation,
suggesting that c-Jun accumulation is a critical event in OA-dependent uPA gene induction. c-Jun protein levels increase significantly
between 100 and 160 min following OA treatment, whereas c-Jun translation increases only slightly in this time frame, suggesting
that post-translation mechanisms are also involved in c-Jun accumulation. Pulse-chase analyses shows that OA specifically
stabilizes c-Jun. We discuss our results with respect to the possibility that protein phosphatase 2A maintains c-Jun in its
down-regulated state in LLC-PK1 cells.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8300623</pmid><doi>10.1016/S0021-9258(17)42025-4</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1994-01, Vol.269 (4), p.2887-2894 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16953859 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 8-Bromo Cyclic Adenosine Monophosphate - pharmacology Animals Base Sequence Binding Sites Biological and medical sciences Blotting, Southern Blotting, Western Cell Line Chloramphenicol O-Acetyltransferase - biosynthesis Chloramphenicol O-Acetyltransferase - metabolism Colchicine - pharmacology Cycloheximide - pharmacology DNA Mutational Analysis DNA-Binding Proteins - metabolism Enzyme Induction Ethers, Cyclic - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic - drug effects Genes, Regulator Homeostasis Kinetics Marine Toxins Molecular and cellular biology Molecular genetics Molecular Sequence Data Nuclear Proteins - isolation & purification Nuclear Proteins - metabolism Okadaic Acid Oligodeoxyribonucleotides - chemistry Oligodeoxyribonucleotides - metabolism Oxazoles - pharmacology Phosphorylation Protein Phosphatase 2 Protein Tyrosine Phosphatases - antagonists & inhibitors Proto-Oncogene Proteins c-jun - biosynthesis Proto-Oncogene Proteins c-jun - metabolism Time Factors Transcription. Transcription factor. Splicing. Rna processing Transcriptional Activation Transfection Urokinase-Type Plasminogen Activator - biosynthesis Urokinase-Type Plasminogen Activator - genetics |
| title | Okadaic acid-dependent induction of the urokinase-type plasminogen activator gene associated with stabilization and autoregulation of c-Jun |
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