Analysis and purification of IgG4 bispecific antibodies by a mixed-mode chromatography

Therapeutic non-hinge-modified IgG4 molecules form bispecific hybrid antibodies with endogenous human IgG4 molecules via a process known as Fab-arm exchange (or called half molecule exchange). Analysis of the bispecific hybrids is critical for studies of half molecule exchange. A number of analytica...

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Veröffentlicht in:	Analytical biochemistry 2015-09, Vol.484, p.173-179
Hauptverfasser:	Yang, Xiaoyu, Zhang, Ying, Wang, Fengqiang, Wang, Larry (Jin), Richardson, Daisy, Shameem, Mohammed, Ambrogelly, Alexandre
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Bispecific - immunology Antibodies, Bispecific - isolation & purification Bispecific antibodies Chromatography - methods Fab-arm exchange Half molecule exchange Humans IgG4 Immunoglobulin G - immunology Kinetics Mixed-mode chromatography
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