Coating with a novel gentamicinpalmitate formulation prevents implant-associated osteomyelitis induced by methicillin-susceptible Staphylococcus aureus in a rat model
Purpose Implant-associated osteomyelitis still represents a demanding challenge due to unfavourable biological conditions, bacterial properties and incremental resistance to antibiotic treatment. Therefore different bactericide or bacteriostatic implant coatings have been developed recently to contr...
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| Veröffentlicht in: | International orthopaedics 2015-05, Vol.39 (5), p.981-988 |
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| creator | Fölsch, Christian Federmann, Maike Kuehn, Klaus D. Kittinger, Clemens Kogler, Stefan Zarfel, Gernot Kerwat, Martina Braun, Steve Fuchs-Winkelmann, Susanne Paletta, Jürgen R. J. Roessler, Philip P. |
| description | Purpose
Implant-associated osteomyelitis still represents a demanding challenge due to unfavourable biological conditions, bacterial properties and incremental resistance to antibiotic treatment. Therefore different bactericide or bacteriostatic implant coatings have been developed recently to control local intramedullary infections. Controlled local release of gentamicin base from a highly lipophilic gentamicin palmitate compound achieves extended intramedullary retention times and thus may improve its bactericide effect.
Methods
Forty male Sprague-Dawley rats were divided into two groups receiving an intramedullary femoral injection of 10
2
colony-forming units (CFU) of a common methicillin susceptible
Staphylococcus aureus
strain (MSSA
Rosenbach
) and either an uncoated femur nail (Group I) or a nail coated with gentamicin palmitate (Group II). Animals were observed for 28 and 42 days. Serum haptoglobin and relative weight gain were assessed as well as rollover cultures of explanted femur nails and histological scores of periprosthetic infection in dissected femurs.
Results
Implants coated with gentamicin palmitate significantly reduced periprosthetic bacterial growth as well as signs of systemic inflammation compared with uncoated implants.
Conclusions
Gentamicin palmitate appears to be a viable coating for the prevention of implant-associated infections. These findings will have to be confirmed in larger animal models as well as in clinical trials. |
| doi_str_mv | 10.1007/s00264-014-2582-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1694982467</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1675878624</sourcerecordid><originalsourceid>FETCH-LOGICAL-c447t-6c94a0698ce8ac2df8cbeff283c2d0a95c74ed8b5098d9010c5c17bcd0c247dc3</originalsourceid><addsrcrecordid>eNqNkc2OFCEYRYnROD2jD-DGsHSDAkVVwdJ0_EsmcaGuCUV91c0EipKfMf1CPqd0enQ5cUWAc28-OAi9YvQto3R8lynlgyCUCcJ7yYl6gnZMdJz0TPVP0Y52ghE-qP4KXed8RykbB8meoyved5IOUu7Q7300xa0H_MuVIzZ4jffg8QHWYoKzbt2MD66YAniJKVTf4LjiLcF9QzJ2YfNmLcTkHK1r2IxjLhDDCbwrrgHrXG07nU44QDm2Su_dSnLNFrbiJg_4WzHb8eSjjdbWjE1NUM_BNk0yBYc4g3-Bni3GZ3j5sN6gHx8_fN9_JrdfP33Zv78lVoixkMEqYeigpAVpLJ8XaSdYFi67tqFG9XYUMMupp0rOijJqe8vGyc7UcjHOtrtBby69W4o_K-Sig2uT-vZIiDVrNiihJBfD-B_o2MtRDlw0lF1Qm2LOCRa9JRdMOmlG9dmkvpjUzaQ-m9SqZV4_1NcpwPwv8VddA_gFyO1qPUDSd7Gmtf3OI61_AAAsrzA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1675878624</pqid></control><display><type>article</type><title>Coating with a novel gentamicinpalmitate formulation prevents implant-associated osteomyelitis induced by methicillin-susceptible Staphylococcus aureus in a rat model</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>SpringerLink Journals - AutoHoldings</source><creator>Fölsch, Christian ; Federmann, Maike ; Kuehn, Klaus D. ; Kittinger, Clemens ; Kogler, Stefan ; Zarfel, Gernot ; Kerwat, Martina ; Braun, Steve ; Fuchs-Winkelmann, Susanne ; Paletta, Jürgen R. J. ; Roessler, Philip P.</creator><creatorcontrib>Fölsch, Christian ; Federmann, Maike ; Kuehn, Klaus D. ; Kittinger, Clemens ; Kogler, Stefan ; Zarfel, Gernot ; Kerwat, Martina ; Braun, Steve ; Fuchs-Winkelmann, Susanne ; Paletta, Jürgen R. J. ; Roessler, Philip P.</creatorcontrib><description>Purpose
Implant-associated osteomyelitis still represents a demanding challenge due to unfavourable biological conditions, bacterial properties and incremental resistance to antibiotic treatment. Therefore different bactericide or bacteriostatic implant coatings have been developed recently to control local intramedullary infections. Controlled local release of gentamicin base from a highly lipophilic gentamicin palmitate compound achieves extended intramedullary retention times and thus may improve its bactericide effect.
Methods
Forty male Sprague-Dawley rats were divided into two groups receiving an intramedullary femoral injection of 10
2
colony-forming units (CFU) of a common methicillin susceptible
Staphylococcus aureus
strain (MSSA
Rosenbach
) and either an uncoated femur nail (Group I) or a nail coated with gentamicin palmitate (Group II). Animals were observed for 28 and 42 days. Serum haptoglobin and relative weight gain were assessed as well as rollover cultures of explanted femur nails and histological scores of periprosthetic infection in dissected femurs.
Results
Implants coated with gentamicin palmitate significantly reduced periprosthetic bacterial growth as well as signs of systemic inflammation compared with uncoated implants.
Conclusions
Gentamicin palmitate appears to be a viable coating for the prevention of implant-associated infections. These findings will have to be confirmed in larger animal models as well as in clinical trials.</description><identifier>ISSN: 0341-2695</identifier><identifier>EISSN: 1432-5195</identifier><identifier>DOI: 10.1007/s00264-014-2582-9</identifier><identifier>PMID: 25380688</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject><![CDATA[Animals ; Anti-Bacterial Agents - administration & dosage ; Anti-Bacterial Agents - therapeutic use ; Antibiotic Prophylaxis - methods ; Coated Materials, Biocompatible - administration & dosage ; Disease Models, Animal ; Gentamicins - administration & dosage ; Male ; Medicine ; Medicine & Public Health ; Original Paper ; Orthopedics ; Osteomyelitis - microbiology ; Osteomyelitis - prevention & control ; Prosthesis-Related Infections - microbiology ; Prosthesis-Related Infections - prevention & control ; Rats, Sprague-Dawley ; Staphylococcal Infections - prevention & control ; Staphylococcus aureus]]></subject><ispartof>International orthopaedics, 2015-05, Vol.39 (5), p.981-988</ispartof><rights>SICOT aisbl 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-6c94a0698ce8ac2df8cbeff283c2d0a95c74ed8b5098d9010c5c17bcd0c247dc3</citedby><cites>FETCH-LOGICAL-c447t-6c94a0698ce8ac2df8cbeff283c2d0a95c74ed8b5098d9010c5c17bcd0c247dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00264-014-2582-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00264-014-2582-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25380688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fölsch, Christian</creatorcontrib><creatorcontrib>Federmann, Maike</creatorcontrib><creatorcontrib>Kuehn, Klaus D.</creatorcontrib><creatorcontrib>Kittinger, Clemens</creatorcontrib><creatorcontrib>Kogler, Stefan</creatorcontrib><creatorcontrib>Zarfel, Gernot</creatorcontrib><creatorcontrib>Kerwat, Martina</creatorcontrib><creatorcontrib>Braun, Steve</creatorcontrib><creatorcontrib>Fuchs-Winkelmann, Susanne</creatorcontrib><creatorcontrib>Paletta, Jürgen R. J.</creatorcontrib><creatorcontrib>Roessler, Philip P.</creatorcontrib><title>Coating with a novel gentamicinpalmitate formulation prevents implant-associated osteomyelitis induced by methicillin-susceptible Staphylococcus aureus in a rat model</title><title>International orthopaedics</title><addtitle>International Orthopaedics (SICOT)</addtitle><addtitle>Int Orthop</addtitle><description>Purpose
Implant-associated osteomyelitis still represents a demanding challenge due to unfavourable biological conditions, bacterial properties and incremental resistance to antibiotic treatment. Therefore different bactericide or bacteriostatic implant coatings have been developed recently to control local intramedullary infections. Controlled local release of gentamicin base from a highly lipophilic gentamicin palmitate compound achieves extended intramedullary retention times and thus may improve its bactericide effect.
Methods
Forty male Sprague-Dawley rats were divided into two groups receiving an intramedullary femoral injection of 10
2
colony-forming units (CFU) of a common methicillin susceptible
Staphylococcus aureus
strain (MSSA
Rosenbach
) and either an uncoated femur nail (Group I) or a nail coated with gentamicin palmitate (Group II). Animals were observed for 28 and 42 days. Serum haptoglobin and relative weight gain were assessed as well as rollover cultures of explanted femur nails and histological scores of periprosthetic infection in dissected femurs.
Results
Implants coated with gentamicin palmitate significantly reduced periprosthetic bacterial growth as well as signs of systemic inflammation compared with uncoated implants.
Conclusions
Gentamicin palmitate appears to be a viable coating for the prevention of implant-associated infections. These findings will have to be confirmed in larger animal models as well as in clinical trials.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - administration & dosage</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Antibiotic Prophylaxis - methods</subject><subject>Coated Materials, Biocompatible - administration & dosage</subject><subject>Disease Models, Animal</subject><subject>Gentamicins - administration & dosage</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original Paper</subject><subject>Orthopedics</subject><subject>Osteomyelitis - microbiology</subject><subject>Osteomyelitis - prevention & control</subject><subject>Prosthesis-Related Infections - microbiology</subject><subject>Prosthesis-Related Infections - prevention & control</subject><subject>Rats, Sprague-Dawley</subject><subject>Staphylococcal Infections - prevention & control</subject><subject>Staphylococcus aureus</subject><issn>0341-2695</issn><issn>1432-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2OFCEYRYnROD2jD-DGsHSDAkVVwdJ0_EsmcaGuCUV91c0EipKfMf1CPqd0enQ5cUWAc28-OAi9YvQto3R8lynlgyCUCcJ7yYl6gnZMdJz0TPVP0Y52ghE-qP4KXed8RykbB8meoyved5IOUu7Q7300xa0H_MuVIzZ4jffg8QHWYoKzbt2MD66YAniJKVTf4LjiLcF9QzJ2YfNmLcTkHK1r2IxjLhDDCbwrrgHrXG07nU44QDm2Su_dSnLNFrbiJg_4WzHb8eSjjdbWjE1NUM_BNk0yBYc4g3-Bni3GZ3j5sN6gHx8_fN9_JrdfP33Zv78lVoixkMEqYeigpAVpLJ8XaSdYFi67tqFG9XYUMMupp0rOijJqe8vGyc7UcjHOtrtBby69W4o_K-Sig2uT-vZIiDVrNiihJBfD-B_o2MtRDlw0lF1Qm2LOCRa9JRdMOmlG9dmkvpjUzaQ-m9SqZV4_1NcpwPwv8VddA_gFyO1qPUDSd7Gmtf3OI61_AAAsrzA</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Fölsch, Christian</creator><creator>Federmann, Maike</creator><creator>Kuehn, Klaus D.</creator><creator>Kittinger, Clemens</creator><creator>Kogler, Stefan</creator><creator>Zarfel, Gernot</creator><creator>Kerwat, Martina</creator><creator>Braun, Steve</creator><creator>Fuchs-Winkelmann, Susanne</creator><creator>Paletta, Jürgen R. J.</creator><creator>Roessler, Philip P.</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7QP</scope><scope>C1K</scope></search><sort><creationdate>20150501</creationdate><title>Coating with a novel gentamicinpalmitate formulation prevents implant-associated osteomyelitis induced by methicillin-susceptible Staphylococcus aureus in a rat model</title><author>Fölsch, Christian ; Federmann, Maike ; Kuehn, Klaus D. ; Kittinger, Clemens ; Kogler, Stefan ; Zarfel, Gernot ; Kerwat, Martina ; Braun, Steve ; Fuchs-Winkelmann, Susanne ; Paletta, Jürgen R. J. ; Roessler, Philip P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-6c94a0698ce8ac2df8cbeff283c2d0a95c74ed8b5098d9010c5c17bcd0c247dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - administration & dosage</topic><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>Antibiotic Prophylaxis - methods</topic><topic>Coated Materials, Biocompatible - administration & dosage</topic><topic>Disease Models, Animal</topic><topic>Gentamicins - administration & dosage</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original Paper</topic><topic>Orthopedics</topic><topic>Osteomyelitis - microbiology</topic><topic>Osteomyelitis - prevention & control</topic><topic>Prosthesis-Related Infections - microbiology</topic><topic>Prosthesis-Related Infections - prevention & control</topic><topic>Rats, Sprague-Dawley</topic><topic>Staphylococcal Infections - prevention & control</topic><topic>Staphylococcus aureus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fölsch, Christian</creatorcontrib><creatorcontrib>Federmann, Maike</creatorcontrib><creatorcontrib>Kuehn, Klaus D.</creatorcontrib><creatorcontrib>Kittinger, Clemens</creatorcontrib><creatorcontrib>Kogler, Stefan</creatorcontrib><creatorcontrib>Zarfel, Gernot</creatorcontrib><creatorcontrib>Kerwat, Martina</creatorcontrib><creatorcontrib>Braun, Steve</creatorcontrib><creatorcontrib>Fuchs-Winkelmann, Susanne</creatorcontrib><creatorcontrib>Paletta, Jürgen R. J.</creatorcontrib><creatorcontrib>Roessler, Philip P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>International orthopaedics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fölsch, Christian</au><au>Federmann, Maike</au><au>Kuehn, Klaus D.</au><au>Kittinger, Clemens</au><au>Kogler, Stefan</au><au>Zarfel, Gernot</au><au>Kerwat, Martina</au><au>Braun, Steve</au><au>Fuchs-Winkelmann, Susanne</au><au>Paletta, Jürgen R. J.</au><au>Roessler, Philip P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coating with a novel gentamicinpalmitate formulation prevents implant-associated osteomyelitis induced by methicillin-susceptible Staphylococcus aureus in a rat model</atitle><jtitle>International orthopaedics</jtitle><stitle>International Orthopaedics (SICOT)</stitle><addtitle>Int Orthop</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>39</volume><issue>5</issue><spage>981</spage><epage>988</epage><pages>981-988</pages><issn>0341-2695</issn><eissn>1432-5195</eissn><abstract>Purpose
Implant-associated osteomyelitis still represents a demanding challenge due to unfavourable biological conditions, bacterial properties and incremental resistance to antibiotic treatment. Therefore different bactericide or bacteriostatic implant coatings have been developed recently to control local intramedullary infections. Controlled local release of gentamicin base from a highly lipophilic gentamicin palmitate compound achieves extended intramedullary retention times and thus may improve its bactericide effect.
Methods
Forty male Sprague-Dawley rats were divided into two groups receiving an intramedullary femoral injection of 10
2
colony-forming units (CFU) of a common methicillin susceptible
Staphylococcus aureus
strain (MSSA
Rosenbach
) and either an uncoated femur nail (Group I) or a nail coated with gentamicin palmitate (Group II). Animals were observed for 28 and 42 days. Serum haptoglobin and relative weight gain were assessed as well as rollover cultures of explanted femur nails and histological scores of periprosthetic infection in dissected femurs.
Results
Implants coated with gentamicin palmitate significantly reduced periprosthetic bacterial growth as well as signs of systemic inflammation compared with uncoated implants.
Conclusions
Gentamicin palmitate appears to be a viable coating for the prevention of implant-associated infections. These findings will have to be confirmed in larger animal models as well as in clinical trials.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25380688</pmid><doi>10.1007/s00264-014-2582-9</doi><tpages>8</tpages></addata></record> |
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| issn | 0341-2695 1432-5195 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerLink Journals - AutoHoldings |
| subjects | Animals Anti-Bacterial Agents - administration & dosage Anti-Bacterial Agents - therapeutic use Antibiotic Prophylaxis - methods Coated Materials, Biocompatible - administration & dosage Disease Models, Animal Gentamicins - administration & dosage Male Medicine Medicine & Public Health Original Paper Orthopedics Osteomyelitis - microbiology Osteomyelitis - prevention & control Prosthesis-Related Infections - microbiology Prosthesis-Related Infections - prevention & control Rats, Sprague-Dawley Staphylococcal Infections - prevention & control Staphylococcus aureus |
| title | Coating with a novel gentamicinpalmitate formulation prevents implant-associated osteomyelitis induced by methicillin-susceptible Staphylococcus aureus in a rat model |
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