Regulation of visfatin by microbial and biomechanical signals in PDL cells
Objectives This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals. Materials and methods PDL cells...
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description | Objectives
This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals.
Materials and methods
PDL cells seeded on BioFlex® plates were exposed to the oral pathogen
Fusobacterium nucleatum
ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NFκB nuclear translocation by immunofluorescence.
Results
F
.
nucleatum
upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the
F
.
nucleatum
-upregulated visfatin expression at 1 day.
F
.
nucleatum
stimulated the NFκB nuclear translocation. Biomechanical loading reduced the stimulatory effects of
F
.
nucleatum
on visfatin expression at 1 and 3 days and also abrogated the
F
.
nucleatum
-induced NFκB nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of
F
.
nucleatum
and/or biomechanical loading on visfatin expression were also observed at protein level.
Conclusions
PDL cells produce visfatin, and this production is enhanced by
F
.
nucleatum
. Biomechanical loading seems to be protective against the effects of
F
.
nucleatum
on visfatin expression.
Clinical relevance
Visfatin produced by periodontal tissues could play a major role in the pathogenesis of periodontitis and the interactions with obesity and other systemic diseases. |
doi_str_mv | 10.1007/s00784-013-0935-1 |
format | Article |
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This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals.
Materials and methods
PDL cells seeded on BioFlex® plates were exposed to the oral pathogen
Fusobacterium nucleatum
ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NFκB nuclear translocation by immunofluorescence.
Results
F
.
nucleatum
upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the
F
.
nucleatum
-upregulated visfatin expression at 1 day.
F
.
nucleatum
stimulated the NFκB nuclear translocation. Biomechanical loading reduced the stimulatory effects of
F
.
nucleatum
on visfatin expression at 1 and 3 days and also abrogated the
F
.
nucleatum
-induced NFκB nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of
F
.
nucleatum
and/or biomechanical loading on visfatin expression were also observed at protein level.
Conclusions
PDL cells produce visfatin, and this production is enhanced by
F
.
nucleatum
. Biomechanical loading seems to be protective against the effects of
F
.
nucleatum
on visfatin expression.
Clinical relevance
Visfatin produced by periodontal tissues could play a major role in the pathogenesis of periodontitis and the interactions with obesity and other systemic diseases.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-013-0935-1</identifier><identifier>PMID: 23404558</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomechanical Phenomena ; Dentistry ; Enzyme-Linked Immunosorbent Assay ; Fusobacterium nucleatum ; Fusobacterium nucleatum - isolation & purification ; Fusobacterium nucleatum - pathogenicity ; Humans ; Medicine ; Nicotinamide Phosphoribosyltransferase - metabolism ; Original Article ; Periodontal Ligament - cytology ; Periodontal Ligament - metabolism ; Periodontal Ligament - microbiology ; Real-Time Polymerase Chain Reaction</subject><ispartof>Clinical oral investigations, 2014-01, Vol.18 (1), p.171-178</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><rights>Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-65b9c8f5334721274b564ac4e12fec26edb86e27d6b10691a942bca9503678f93</citedby><cites>FETCH-LOGICAL-c471t-65b9c8f5334721274b564ac4e12fec26edb86e27d6b10691a942bca9503678f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-013-0935-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-013-0935-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23404558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nogueira, Andressa Vilas Boas</creatorcontrib><creatorcontrib>Nokhbehsaim, Marjan</creatorcontrib><creatorcontrib>Eick, Sigrun</creatorcontrib><creatorcontrib>Bourauel, Christoph</creatorcontrib><creatorcontrib>Jäger, Andreas</creatorcontrib><creatorcontrib>Jepsen, Søren</creatorcontrib><creatorcontrib>Cirelli, Joni Augusto</creatorcontrib><creatorcontrib>Deschner, James</creatorcontrib><title>Regulation of visfatin by microbial and biomechanical signals in PDL cells</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals.
Materials and methods
PDL cells seeded on BioFlex® plates were exposed to the oral pathogen
Fusobacterium nucleatum
ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NFκB nuclear translocation by immunofluorescence.
Results
F
.
nucleatum
upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the
F
.
nucleatum
-upregulated visfatin expression at 1 day.
F
.
nucleatum
stimulated the NFκB nuclear translocation. Biomechanical loading reduced the stimulatory effects of
F
.
nucleatum
on visfatin expression at 1 and 3 days and also abrogated the
F
.
nucleatum
-induced NFκB nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of
F
.
nucleatum
and/or biomechanical loading on visfatin expression were also observed at protein level.
Conclusions
PDL cells produce visfatin, and this production is enhanced by
F
.
nucleatum
. Biomechanical loading seems to be protective against the effects of
F
.
nucleatum
on visfatin expression.
Clinical relevance
Visfatin produced by periodontal tissues could play a major role in the pathogenesis of periodontitis and the interactions with obesity and other systemic diseases.</description><subject>Biomechanical Phenomena</subject><subject>Dentistry</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fusobacterium nucleatum</subject><subject>Fusobacterium nucleatum - isolation & purification</subject><subject>Fusobacterium nucleatum - pathogenicity</subject><subject>Humans</subject><subject>Medicine</subject><subject>Nicotinamide Phosphoribosyltransferase - metabolism</subject><subject>Original Article</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - metabolism</subject><subject>Periodontal Ligament - microbiology</subject><subject>Real-Time Polymerase Chain Reaction</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU1PAyEQhonR2Fr9AV7MJl68rDLAwnI0fpsmGqNnApStNPtRl65J_72sW40xMV4YGJ55GeZF6BDwKWAszkJccpZioCmWNEthC42BUZ5SIWD7c09SLnMYob0QFhgD44LuohGhDLMsy8fo_snNu1KvfFMnTZG8-1DEQ52YdVJ52zbG6zLR9SwxvqmcfdW1tzET_LzWZUgi-Xg5Tawry7CPdoqYcwebOEEv11fPF7fp9OHm7uJ8mlomYJXyzEibFxmlTBAggpmMM22ZA1I4S7ibmZw7ImbcAOYStGTEWC0zTLnIC0kn6GTQXbbNW-fCSlU-9B3o2jVdUMAlk5xzKf5HmYwTzDiHiB7_QhdN1_afjJSgFAua94IwUHE0IbSuUMvWV7pdK8Cq90QNnqjoieo9Ub3y0Ua5M5WbfVd8mRABMgAhXtVz1_54-k_VDyZ6lCs</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Nogueira, Andressa Vilas Boas</creator><creator>Nokhbehsaim, Marjan</creator><creator>Eick, Sigrun</creator><creator>Bourauel, Christoph</creator><creator>Jäger, Andreas</creator><creator>Jepsen, Søren</creator><creator>Cirelli, Joni Augusto</creator><creator>Deschner, James</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20140101</creationdate><title>Regulation of visfatin by microbial and biomechanical signals in PDL cells</title><author>Nogueira, Andressa Vilas Boas ; Nokhbehsaim, Marjan ; Eick, Sigrun ; Bourauel, Christoph ; Jäger, Andreas ; Jepsen, Søren ; Cirelli, Joni Augusto ; Deschner, James</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-65b9c8f5334721274b564ac4e12fec26edb86e27d6b10691a942bca9503678f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biomechanical Phenomena</topic><topic>Dentistry</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fusobacterium nucleatum</topic><topic>Fusobacterium nucleatum - isolation & purification</topic><topic>Fusobacterium nucleatum - pathogenicity</topic><topic>Humans</topic><topic>Medicine</topic><topic>Nicotinamide Phosphoribosyltransferase - metabolism</topic><topic>Original Article</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - metabolism</topic><topic>Periodontal Ligament - microbiology</topic><topic>Real-Time Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nogueira, Andressa Vilas Boas</creatorcontrib><creatorcontrib>Nokhbehsaim, Marjan</creatorcontrib><creatorcontrib>Eick, Sigrun</creatorcontrib><creatorcontrib>Bourauel, Christoph</creatorcontrib><creatorcontrib>Jäger, Andreas</creatorcontrib><creatorcontrib>Jepsen, Søren</creatorcontrib><creatorcontrib>Cirelli, Joni Augusto</creatorcontrib><creatorcontrib>Deschner, James</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nogueira, Andressa Vilas Boas</au><au>Nokhbehsaim, Marjan</au><au>Eick, Sigrun</au><au>Bourauel, Christoph</au><au>Jäger, Andreas</au><au>Jepsen, Søren</au><au>Cirelli, Joni Augusto</au><au>Deschner, James</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of visfatin by microbial and biomechanical signals in PDL cells</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>18</volume><issue>1</issue><spage>171</spage><epage>178</epage><pages>171-178</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals.
Materials and methods
PDL cells seeded on BioFlex® plates were exposed to the oral pathogen
Fusobacterium nucleatum
ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NFκB nuclear translocation by immunofluorescence.
Results
F
.
nucleatum
upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the
F
.
nucleatum
-upregulated visfatin expression at 1 day.
F
.
nucleatum
stimulated the NFκB nuclear translocation. Biomechanical loading reduced the stimulatory effects of
F
.
nucleatum
on visfatin expression at 1 and 3 days and also abrogated the
F
.
nucleatum
-induced NFκB nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of
F
.
nucleatum
and/or biomechanical loading on visfatin expression were also observed at protein level.
Conclusions
PDL cells produce visfatin, and this production is enhanced by
F
.
nucleatum
. Biomechanical loading seems to be protective against the effects of
F
.
nucleatum
on visfatin expression.
Clinical relevance
Visfatin produced by periodontal tissues could play a major role in the pathogenesis of periodontitis and the interactions with obesity and other systemic diseases.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>23404558</pmid><doi>10.1007/s00784-013-0935-1</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Biomechanical Phenomena Dentistry Enzyme-Linked Immunosorbent Assay Fusobacterium nucleatum Fusobacterium nucleatum - isolation & purification Fusobacterium nucleatum - pathogenicity Humans Medicine Nicotinamide Phosphoribosyltransferase - metabolism Original Article Periodontal Ligament - cytology Periodontal Ligament - metabolism Periodontal Ligament - microbiology Real-Time Polymerase Chain Reaction |
title | Regulation of visfatin by microbial and biomechanical signals in PDL cells |
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