Regulation of visfatin by microbial and biomechanical signals in PDL cells

Objectives This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals. Materials and methods PDL cells...

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Veröffentlicht in:Clinical oral investigations 2014-01, Vol.18 (1), p.171-178
Hauptverfasser: Nogueira, Andressa Vilas Boas, Nokhbehsaim, Marjan, Eick, Sigrun, Bourauel, Christoph, Jäger, Andreas, Jepsen, Søren, Cirelli, Joni Augusto, Deschner, James
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container_issue 1
container_start_page 171
container_title Clinical oral investigations
container_volume 18
creator Nogueira, Andressa Vilas Boas
Nokhbehsaim, Marjan
Eick, Sigrun
Bourauel, Christoph
Jäger, Andreas
Jepsen, Søren
Cirelli, Joni Augusto
Deschner, James
description Objectives This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals. Materials and methods PDL cells seeded on BioFlex® plates were exposed to the oral pathogen Fusobacterium nucleatum ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NFκB nuclear translocation by immunofluorescence. Results F . nucleatum upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the F . nucleatum -upregulated visfatin expression at 1 day. F . nucleatum stimulated the NFκB nuclear translocation. Biomechanical loading reduced the stimulatory effects of F . nucleatum on visfatin expression at 1 and 3 days and also abrogated the F . nucleatum -induced NFκB nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of F . nucleatum and/or biomechanical loading on visfatin expression were also observed at protein level. Conclusions PDL cells produce visfatin, and this production is enhanced by F . nucleatum . Biomechanical loading seems to be protective against the effects of F . nucleatum on visfatin expression. Clinical relevance Visfatin produced by periodontal tissues could play a major role in the pathogenesis of periodontitis and the interactions with obesity and other systemic diseases.
doi_str_mv 10.1007/s00784-013-0935-1
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Materials and methods PDL cells seeded on BioFlex® plates were exposed to the oral pathogen Fusobacterium nucleatum ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NFκB nuclear translocation by immunofluorescence. Results F . nucleatum upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the F . nucleatum -upregulated visfatin expression at 1 day. F . nucleatum stimulated the NFκB nuclear translocation. Biomechanical loading reduced the stimulatory effects of F . nucleatum on visfatin expression at 1 and 3 days and also abrogated the F . nucleatum -induced NFκB nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of F . nucleatum and/or biomechanical loading on visfatin expression were also observed at protein level. Conclusions PDL cells produce visfatin, and this production is enhanced by F . nucleatum . Biomechanical loading seems to be protective against the effects of F . nucleatum on visfatin expression. Clinical relevance Visfatin produced by periodontal tissues could play a major role in the pathogenesis of periodontitis and the interactions with obesity and other systemic diseases.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-013-0935-1</identifier><identifier>PMID: 23404558</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomechanical Phenomena ; Dentistry ; Enzyme-Linked Immunosorbent Assay ; Fusobacterium nucleatum ; Fusobacterium nucleatum - isolation &amp; purification ; Fusobacterium nucleatum - pathogenicity ; Humans ; Medicine ; Nicotinamide Phosphoribosyltransferase - metabolism ; Original Article ; Periodontal Ligament - cytology ; Periodontal Ligament - metabolism ; Periodontal Ligament - microbiology ; Real-Time Polymerase Chain Reaction</subject><ispartof>Clinical oral investigations, 2014-01, Vol.18 (1), p.171-178</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><rights>Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-65b9c8f5334721274b564ac4e12fec26edb86e27d6b10691a942bca9503678f93</citedby><cites>FETCH-LOGICAL-c471t-65b9c8f5334721274b564ac4e12fec26edb86e27d6b10691a942bca9503678f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-013-0935-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-013-0935-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23404558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nogueira, Andressa Vilas Boas</creatorcontrib><creatorcontrib>Nokhbehsaim, Marjan</creatorcontrib><creatorcontrib>Eick, Sigrun</creatorcontrib><creatorcontrib>Bourauel, Christoph</creatorcontrib><creatorcontrib>Jäger, Andreas</creatorcontrib><creatorcontrib>Jepsen, Søren</creatorcontrib><creatorcontrib>Cirelli, Joni Augusto</creatorcontrib><creatorcontrib>Deschner, James</creatorcontrib><title>Regulation of visfatin by microbial and biomechanical signals in PDL cells</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals. Materials and methods PDL cells seeded on BioFlex® plates were exposed to the oral pathogen Fusobacterium nucleatum ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NFκB nuclear translocation by immunofluorescence. Results F . nucleatum upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the F . nucleatum -upregulated visfatin expression at 1 day. F . nucleatum stimulated the NFκB nuclear translocation. Biomechanical loading reduced the stimulatory effects of F . nucleatum on visfatin expression at 1 and 3 days and also abrogated the F . nucleatum -induced NFκB nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of F . nucleatum and/or biomechanical loading on visfatin expression were also observed at protein level. Conclusions PDL cells produce visfatin, and this production is enhanced by F . nucleatum . Biomechanical loading seems to be protective against the effects of F . nucleatum on visfatin expression. 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Materials and methods PDL cells seeded on BioFlex® plates were exposed to the oral pathogen Fusobacterium nucleatum ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NFκB nuclear translocation by immunofluorescence. Results F . nucleatum upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the F . nucleatum -upregulated visfatin expression at 1 day. F . nucleatum stimulated the NFκB nuclear translocation. Biomechanical loading reduced the stimulatory effects of F . nucleatum on visfatin expression at 1 and 3 days and also abrogated the F . nucleatum -induced NFκB nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of F . nucleatum and/or biomechanical loading on visfatin expression were also observed at protein level. Conclusions PDL cells produce visfatin, and this production is enhanced by F . nucleatum . Biomechanical loading seems to be protective against the effects of F . nucleatum on visfatin expression. Clinical relevance Visfatin produced by periodontal tissues could play a major role in the pathogenesis of periodontitis and the interactions with obesity and other systemic diseases.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>23404558</pmid><doi>10.1007/s00784-013-0935-1</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
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subjects Biomechanical Phenomena
Dentistry
Enzyme-Linked Immunosorbent Assay
Fusobacterium nucleatum
Fusobacterium nucleatum - isolation & purification
Fusobacterium nucleatum - pathogenicity
Humans
Medicine
Nicotinamide Phosphoribosyltransferase - metabolism
Original Article
Periodontal Ligament - cytology
Periodontal Ligament - metabolism
Periodontal Ligament - microbiology
Real-Time Polymerase Chain Reaction
title Regulation of visfatin by microbial and biomechanical signals in PDL cells
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