Improved recovery of chlorine-stressed coliforms with pyruvate supplemented media
The use of sodium pyruvate in lauryl sulphate-based enumeration media to facilitate the recovery of chlorine-stressed coliforms was investigated as part of a programme to develop a single membrane enumeration medium for coliforms and Escherichia coli. Pyruvate degraded peroxides and promoted cell re...
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| Veröffentlicht in: | Water science and technology 1995, Vol.31 (5-6), p.255-258 |
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| creator | Sartory, D.P. |
| description | The use of sodium pyruvate in lauryl sulphate-based enumeration media to facilitate the recovery of chlorine-stressed coliforms was investigated as part of a programme to develop a single membrane enumeration medium for coliforms and Escherichia coli. Pyruvate degraded peroxides and promoted cell recovery. Water containing 0.1 mg free chlorine per litre was inoculated with E. coli and enumerations were made at various times with the standard medium supplemented with 0.01-1.0 per cent pyruvate. There was a 9-fold increase in recovery of bacteria from samples exposed to chlorine for 3 h with media containing 0.05 per cent pyruvate or above. Measurements on a range of chlorinated and unchlorinated water samples also demonstrated higher counts on chlorinated waters with the 0.05 per cent pyruvate-modified medium. |
| doi_str_mv | 10.1016/0273-1223(95)00276-S |
| format | Article |
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Pyruvate degraded peroxides and promoted cell recovery. Water containing 0.1 mg free chlorine per litre was inoculated with E. coli and enumerations were made at various times with the standard medium supplemented with 0.01-1.0 per cent pyruvate. There was a 9-fold increase in recovery of bacteria from samples exposed to chlorine for 3 h with media containing 0.05 per cent pyruvate or above. Measurements on a range of chlorinated and unchlorinated water samples also demonstrated higher counts on chlorinated waters with the 0.05 per cent pyruvate-modified medium.</description><identifier>ISSN: 0273-1223</identifier><identifier>ISBN: 9780080426556</identifier><identifier>ISBN: 0080426557</identifier><identifier>EISSN: 1996-9732</identifier><identifier>DOI: 10.1016/0273-1223(95)00276-S</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>bacteriological water quality ; chlorinated water ; Chlorine ; Coliforms ; disinfection ; E coli ; Enumeration ; Indicator bacteria ; Membrane filtration ; Pyruvic acid ; Recovery ; Sodium ; Sodium pyruvate</subject><ispartof>Water science and technology, 1995, Vol.31 (5-6), p.255-258</ispartof><rights>1995 International Association on Water Quality</rights><rights>Copyright IWA Publishing Mar 1995</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417s-a9c07c4702de17031722534a5b4f5f603527ea4878239596ecfed86bc72120363</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,4010,4036,4037,27900,27901,27902</link.rule.ids></links><search><contributor>Grabow, WOK</contributor><contributor>Morris, R</contributor><contributor>Botzenhart</contributor><creatorcontrib>Sartory, D.P.</creatorcontrib><title>Improved recovery of chlorine-stressed coliforms with pyruvate supplemented media</title><title>Water science and technology</title><description>The use of sodium pyruvate in lauryl sulphate-based enumeration media to facilitate the recovery of chlorine-stressed coliforms was investigated as part of a programme to develop a single membrane enumeration medium for coliforms and Escherichia coli. Pyruvate degraded peroxides and promoted cell recovery. Water containing 0.1 mg free chlorine per litre was inoculated with E. coli and enumerations were made at various times with the standard medium supplemented with 0.01-1.0 per cent pyruvate. There was a 9-fold increase in recovery of bacteria from samples exposed to chlorine for 3 h with media containing 0.05 per cent pyruvate or above. Measurements on a range of chlorinated and unchlorinated water samples also demonstrated higher counts on chlorinated waters with the 0.05 per cent pyruvate-modified medium.</description><subject>bacteriological water quality</subject><subject>chlorinated water</subject><subject>Chlorine</subject><subject>Coliforms</subject><subject>disinfection</subject><subject>E coli</subject><subject>Enumeration</subject><subject>Indicator bacteria</subject><subject>Membrane filtration</subject><subject>Pyruvic acid</subject><subject>Recovery</subject><subject>Sodium</subject><subject>Sodium 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| identifier | ISSN: 0273-1223 |
| ispartof | Water science and technology, 1995, Vol.31 (5-6), p.255-258 |
| issn | 0273-1223 1996-9732 |
| language | eng |
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| source | EZB-FREE-00999 freely available EZB journals |
| subjects | bacteriological water quality chlorinated water Chlorine Coliforms disinfection E coli Enumeration Indicator bacteria Membrane filtration Pyruvic acid Recovery Sodium Sodium pyruvate |
| title | Improved recovery of chlorine-stressed coliforms with pyruvate supplemented media |
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