The low-affinity nerve growth factor receptor p75NTR identifies a transient stem cell-like state in oral squamous cell carcinoma cells
Background Although several markers have been used for enrichment of cells with stem cell‐like properties in oral squamous cell carcinoma (OSCC), isolation of a pure subpopulation is still a challenging task. Normal oral and esophageal keratinocyte stem cells have been previously isolated using the...
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| Veröffentlicht in: | Journal of oral pathology & medicine 2015-07, Vol.44 (6), p.410-419 |
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| container_title | Journal of oral pathology & medicine |
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| creator | Osman, Tarig A. Parajuli, Himalaya Sapkota, Dipak Ahmed, Israa A. H. Johannessen, Anne Ch Costea, Daniela Elena |
| description | Background
Although several markers have been used for enrichment of cells with stem cell‐like properties in oral squamous cell carcinoma (OSCC), isolation of a pure subpopulation is still a challenging task. Normal oral and esophageal keratinocyte stem cells have been previously isolated using the low‐affinity nerve growth factor receptor p75NTR.
Objective
To investigate the potential of p75NTR as a marker for identification and isolation of oral cancer cells with stem cell‐like properties.
Methods
Subpopulations of cells with high or low expression of p75NTR were sorted from OSCC‐derived cells and compared for sphere/colony formation, in vivo tumor formation ability, expression of stem cell‐related molecules, cell cycle distribution and drug resistance.
Results
p75NTRHigh cells exhibited statistically significant higher stem cell properties than p75NTRLow cells in all assays performed. Nevertheless, p75NTRLow subpopulation did also exhibit some stem cell features, but to a lesser extent. Propagation of p75NTRLow cells for several passages in culture showed that the expression of p75NTR could rise spontaneously. This finding was also supported by the similar expression of p75NTR by the xenografts generated by both subpopulations in NOD\SCID IL2Rgnullmice.
Conclusion
p75NTR can be used for isolating a subpopulation enriched for cells with stem cell‐like properties in OSCC. De novo generation of p75NTRHigh cells from p75NTRLow cells suggests either that there is another subpopulation with stem cell features within the p75NTRLow cells, or that the p75NTRLow cells can dedifferentiate due to a contextually regulated equilibrium between stem cell‐like cells and transit‐amplifying neoplastic progenitors. |
| doi_str_mv | 10.1111/jop.12251 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1693719582</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1693719582</sourcerecordid><originalsourceid>FETCH-LOGICAL-i3941-825360768651466d870a49bda062dcbed97adb405f1e6c86dd6803d98195e6153</originalsourceid><addsrcrecordid>eNo9kU1PGzEQhq2qiKSUQ_9A5WMvSzzetb17rFCTgqKAShBHy1nPNob9wt405A_0d-MkwFw8H89rjeYl5BuwC4gxeez6C-BcwCcyBslYwhRkn8mYFSxLYpuPyJcQHhkDlWZwSkZccOBKqDH5v1wjrbttYqrKtW7Y0Rb9P6R_fbcd1rQy5dB56rHEfp_0SiyWf6iz2A6uchiooYM3bXCxQcOADS2xrpPaPWEszYDUtbTzpqbheWOabhMOAC2NL13bNeZQhq_kpDJ1wPO394zcT38tL38n85vZ1eXPeeLSIoMk5yKVTMlcCsiktLliJitW1jDJbblCWyhjVxkTFaAsc2mtzFlqixwKgRJEekZ-HP_tffe8wTDoxoX9BqbFuJsGWaQqwjmP6Pc3dLNq0Oreu8b4nX6_XQQmR2Dratx9zIHpvSk6mqIPpujrm9tDEhXJUeHipV4-FMY_aalSJfTDYqZvYX43zWZTfZe-Ahvljo8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1693719582</pqid></control><display><type>article</type><title>The low-affinity nerve growth factor receptor p75NTR identifies a transient stem cell-like state in oral squamous cell carcinoma cells</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Osman, Tarig A. ; Parajuli, Himalaya ; Sapkota, Dipak ; Ahmed, Israa A. H. ; Johannessen, Anne Ch ; Costea, Daniela Elena</creator><creatorcontrib>Osman, Tarig A. ; Parajuli, Himalaya ; Sapkota, Dipak ; Ahmed, Israa A. H. ; Johannessen, Anne Ch ; Costea, Daniela Elena</creatorcontrib><description>Background
Although several markers have been used for enrichment of cells with stem cell‐like properties in oral squamous cell carcinoma (OSCC), isolation of a pure subpopulation is still a challenging task. Normal oral and esophageal keratinocyte stem cells have been previously isolated using the low‐affinity nerve growth factor receptor p75NTR.
Objective
To investigate the potential of p75NTR as a marker for identification and isolation of oral cancer cells with stem cell‐like properties.
Methods
Subpopulations of cells with high or low expression of p75NTR were sorted from OSCC‐derived cells and compared for sphere/colony formation, in vivo tumor formation ability, expression of stem cell‐related molecules, cell cycle distribution and drug resistance.
Results
p75NTRHigh cells exhibited statistically significant higher stem cell properties than p75NTRLow cells in all assays performed. Nevertheless, p75NTRLow subpopulation did also exhibit some stem cell features, but to a lesser extent. Propagation of p75NTRLow cells for several passages in culture showed that the expression of p75NTR could rise spontaneously. This finding was also supported by the similar expression of p75NTR by the xenografts generated by both subpopulations in NOD\SCID IL2Rgnullmice.
Conclusion
p75NTR can be used for isolating a subpopulation enriched for cells with stem cell‐like properties in OSCC. De novo generation of p75NTRHigh cells from p75NTRLow cells suggests either that there is another subpopulation with stem cell features within the p75NTRLow cells, or that the p75NTRLow cells can dedifferentiate due to a contextually regulated equilibrium between stem cell‐like cells and transit‐amplifying neoplastic progenitors.</description><identifier>ISSN: 0904-2512</identifier><identifier>EISSN: 1600-0714</identifier><identifier>DOI: 10.1111/jop.12251</identifier><identifier>PMID: 25212757</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Animals ; Biomarkers, Tumor - metabolism ; cancer stem cell ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Cell Cycle - physiology ; Cell Line, Tumor ; Dentistry ; Head and Neck Neoplasms - metabolism ; Head and Neck Neoplasms - pathology ; Heterografts ; Humans ; Immunohistochemistry - methods ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Mouth Neoplasms - metabolism ; Mouth Neoplasms - pathology ; Neoplastic Stem Cells - metabolism ; Neoplastic Stem Cells - pathology ; Nerve Tissue Proteins - metabolism ; oral squamous cell carcinoma ; p75NTR ; plasticity ; Receptors, Nerve Growth Factor - metabolism ; Squamous Cell Carcinoma of Head and Neck ; stochastic stem cell ; tumor heterogeneity</subject><ispartof>Journal of oral pathology & medicine, 2015-07, Vol.44 (6), p.410-419</ispartof><rights>2014 The Authors. Published by John Wiley & Sons Ltd</rights><rights>2014 The Authors. Journal of Oral Pathology & Medicine Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjop.12251$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjop.12251$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25212757$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Osman, Tarig A.</creatorcontrib><creatorcontrib>Parajuli, Himalaya</creatorcontrib><creatorcontrib>Sapkota, Dipak</creatorcontrib><creatorcontrib>Ahmed, Israa A. H.</creatorcontrib><creatorcontrib>Johannessen, Anne Ch</creatorcontrib><creatorcontrib>Costea, Daniela Elena</creatorcontrib><title>The low-affinity nerve growth factor receptor p75NTR identifies a transient stem cell-like state in oral squamous cell carcinoma cells</title><title>Journal of oral pathology & medicine</title><addtitle>J Oral Pathol Med</addtitle><description>Background
Although several markers have been used for enrichment of cells with stem cell‐like properties in oral squamous cell carcinoma (OSCC), isolation of a pure subpopulation is still a challenging task. Normal oral and esophageal keratinocyte stem cells have been previously isolated using the low‐affinity nerve growth factor receptor p75NTR.
Objective
To investigate the potential of p75NTR as a marker for identification and isolation of oral cancer cells with stem cell‐like properties.
Methods
Subpopulations of cells with high or low expression of p75NTR were sorted from OSCC‐derived cells and compared for sphere/colony formation, in vivo tumor formation ability, expression of stem cell‐related molecules, cell cycle distribution and drug resistance.
Results
p75NTRHigh cells exhibited statistically significant higher stem cell properties than p75NTRLow cells in all assays performed. Nevertheless, p75NTRLow subpopulation did also exhibit some stem cell features, but to a lesser extent. Propagation of p75NTRLow cells for several passages in culture showed that the expression of p75NTR could rise spontaneously. This finding was also supported by the similar expression of p75NTR by the xenografts generated by both subpopulations in NOD\SCID IL2Rgnullmice.
Conclusion
p75NTR can be used for isolating a subpopulation enriched for cells with stem cell‐like properties in OSCC. De novo generation of p75NTRHigh cells from p75NTRLow cells suggests either that there is another subpopulation with stem cell features within the p75NTRLow cells, or that the p75NTRLow cells can dedifferentiate due to a contextually regulated equilibrium between stem cell‐like cells and transit‐amplifying neoplastic progenitors.</description><subject>Animals</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>cancer stem cell</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell Cycle - physiology</subject><subject>Cell Line, Tumor</subject><subject>Dentistry</subject><subject>Head and Neck Neoplasms - metabolism</subject><subject>Head and Neck Neoplasms - pathology</subject><subject>Heterografts</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Mouth Neoplasms - metabolism</subject><subject>Mouth Neoplasms - pathology</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>oral squamous cell carcinoma</subject><subject>p75NTR</subject><subject>plasticity</subject><subject>Receptors, Nerve Growth Factor - metabolism</subject><subject>Squamous Cell Carcinoma of Head and Neck</subject><subject>stochastic stem cell</subject><subject>tumor heterogeneity</subject><issn>0904-2512</issn><issn>1600-0714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNo9kU1PGzEQhq2qiKSUQ_9A5WMvSzzetb17rFCTgqKAShBHy1nPNob9wt405A_0d-MkwFw8H89rjeYl5BuwC4gxeez6C-BcwCcyBslYwhRkn8mYFSxLYpuPyJcQHhkDlWZwSkZccOBKqDH5v1wjrbttYqrKtW7Y0Rb9P6R_fbcd1rQy5dB56rHEfp_0SiyWf6iz2A6uchiooYM3bXCxQcOADS2xrpPaPWEszYDUtbTzpqbheWOabhMOAC2NL13bNeZQhq_kpDJ1wPO394zcT38tL38n85vZ1eXPeeLSIoMk5yKVTMlcCsiktLliJitW1jDJbblCWyhjVxkTFaAsc2mtzFlqixwKgRJEekZ-HP_tffe8wTDoxoX9BqbFuJsGWaQqwjmP6Pc3dLNq0Oreu8b4nX6_XQQmR2Dratx9zIHpvSk6mqIPpujrm9tDEhXJUeHipV4-FMY_aalSJfTDYqZvYX43zWZTfZe-Ahvljo8</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Osman, Tarig A.</creator><creator>Parajuli, Himalaya</creator><creator>Sapkota, Dipak</creator><creator>Ahmed, Israa A. H.</creator><creator>Johannessen, Anne Ch</creator><creator>Costea, Daniela Elena</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201507</creationdate><title>The low-affinity nerve growth factor receptor p75NTR identifies a transient stem cell-like state in oral squamous cell carcinoma cells</title><author>Osman, Tarig A. ; Parajuli, Himalaya ; Sapkota, Dipak ; Ahmed, Israa A. H. ; Johannessen, Anne Ch ; Costea, Daniela Elena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3941-825360768651466d870a49bda062dcbed97adb405f1e6c86dd6803d98195e6153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>cancer stem cell</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Cell Cycle - physiology</topic><topic>Cell Line, Tumor</topic><topic>Dentistry</topic><topic>Head and Neck Neoplasms - metabolism</topic><topic>Head and Neck Neoplasms - pathology</topic><topic>Heterografts</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Mice</topic><topic>Mice, Inbred NOD</topic><topic>Mice, SCID</topic><topic>Mouth Neoplasms - metabolism</topic><topic>Mouth Neoplasms - pathology</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>oral squamous cell carcinoma</topic><topic>p75NTR</topic><topic>plasticity</topic><topic>Receptors, Nerve Growth Factor - metabolism</topic><topic>Squamous Cell Carcinoma of Head and Neck</topic><topic>stochastic stem cell</topic><topic>tumor heterogeneity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osman, Tarig A.</creatorcontrib><creatorcontrib>Parajuli, Himalaya</creatorcontrib><creatorcontrib>Sapkota, Dipak</creatorcontrib><creatorcontrib>Ahmed, Israa A. H.</creatorcontrib><creatorcontrib>Johannessen, Anne Ch</creatorcontrib><creatorcontrib>Costea, Daniela Elena</creatorcontrib><collection>Istex</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of oral pathology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osman, Tarig A.</au><au>Parajuli, Himalaya</au><au>Sapkota, Dipak</au><au>Ahmed, Israa A. H.</au><au>Johannessen, Anne Ch</au><au>Costea, Daniela Elena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The low-affinity nerve growth factor receptor p75NTR identifies a transient stem cell-like state in oral squamous cell carcinoma cells</atitle><jtitle>Journal of oral pathology & medicine</jtitle><addtitle>J Oral Pathol Med</addtitle><date>2015-07</date><risdate>2015</risdate><volume>44</volume><issue>6</issue><spage>410</spage><epage>419</epage><pages>410-419</pages><issn>0904-2512</issn><eissn>1600-0714</eissn><abstract>Background
Although several markers have been used for enrichment of cells with stem cell‐like properties in oral squamous cell carcinoma (OSCC), isolation of a pure subpopulation is still a challenging task. Normal oral and esophageal keratinocyte stem cells have been previously isolated using the low‐affinity nerve growth factor receptor p75NTR.
Objective
To investigate the potential of p75NTR as a marker for identification and isolation of oral cancer cells with stem cell‐like properties.
Methods
Subpopulations of cells with high or low expression of p75NTR were sorted from OSCC‐derived cells and compared for sphere/colony formation, in vivo tumor formation ability, expression of stem cell‐related molecules, cell cycle distribution and drug resistance.
Results
p75NTRHigh cells exhibited statistically significant higher stem cell properties than p75NTRLow cells in all assays performed. Nevertheless, p75NTRLow subpopulation did also exhibit some stem cell features, but to a lesser extent. Propagation of p75NTRLow cells for several passages in culture showed that the expression of p75NTR could rise spontaneously. This finding was also supported by the similar expression of p75NTR by the xenografts generated by both subpopulations in NOD\SCID IL2Rgnullmice.
Conclusion
p75NTR can be used for isolating a subpopulation enriched for cells with stem cell‐like properties in OSCC. De novo generation of p75NTRHigh cells from p75NTRLow cells suggests either that there is another subpopulation with stem cell features within the p75NTRLow cells, or that the p75NTRLow cells can dedifferentiate due to a contextually regulated equilibrium between stem cell‐like cells and transit‐amplifying neoplastic progenitors.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>25212757</pmid><doi>10.1111/jop.12251</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biomarkers, Tumor - metabolism cancer stem cell Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Cell Cycle - physiology Cell Line, Tumor Dentistry Head and Neck Neoplasms - metabolism Head and Neck Neoplasms - pathology Heterografts Humans Immunohistochemistry - methods Mice Mice, Inbred NOD Mice, SCID Mouth Neoplasms - metabolism Mouth Neoplasms - pathology Neoplastic Stem Cells - metabolism Neoplastic Stem Cells - pathology Nerve Tissue Proteins - metabolism oral squamous cell carcinoma p75NTR plasticity Receptors, Nerve Growth Factor - metabolism Squamous Cell Carcinoma of Head and Neck stochastic stem cell tumor heterogeneity |
| title | The low-affinity nerve growth factor receptor p75NTR identifies a transient stem cell-like state in oral squamous cell carcinoma cells |
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