Single active‐site mutants are sufficient to enhance serine:pyruvate α‐transaminase activity in an ω‐transaminase
We have analyzed the natural evolution of transaminase structure and sequence between an α‐transaminase serine‐pyruvate aminotransferase and an ω‐transaminase from Chromobacterium violaceum with
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| Veröffentlicht in: | The FEBS journal 2015-07, Vol.282 (13), p.2512-2526 |
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| container_issue | 13 |
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| container_title | The FEBS journal |
| container_volume | 282 |
| creator | Deszcz, Dawid Affaticati, Pierre Ladkau, Nadine Gegel, Alex Ward, John M Hailes, Helen C Dalby, Paul A |
| description | We have analyzed the natural evolution of transaminase structure and sequence between an α‐transaminase serine‐pyruvate aminotransferase and an ω‐transaminase from Chromobacterium violaceum with |
| doi_str_mv | 10.1111/febs.13293 |
| format | Article |
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We also show that these structural changes correlate strongly with transaminase substrate specificity during evolution and therefore might normally be presumed to be essential determinants of substrate specificity. However, key residues are often conserved spatially during evolution and yet originate from within a different region of the sequence via structural reorganizations. In the present study, we also show that α‐transaminase‐type serine‐pyruvate aminotransferase activity can be engineered into the CV2025 ω‐transaminase scaffold with any one of many possible single‐point mutations at three key positions, without the requirement for significant backbone remodeling, or repositioning of the residue from a different region of sequence. This finding has significant implications for enzyme redesign in which solutions to substrate specificity changes may be found more efficiently than is achieved by engineering in all sequence and structure determinants identified by correlation to substrate specificity.</description><identifier>ISSN: 1742-464X</identifier><identifier>EISSN: 1742-4658</identifier><identifier>DOI: 10.1111/febs.13293</identifier><identifier>PMID: 25846556</identifier><language>eng</language><publisher>England: Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies</publisher><subject>active sites ; aminotransferase ; biocatalysis ; Catalytic Domain ; Chromobacterium violaceum ; directed evolution ; engineering ; evolution ; High-Throughput Screening Assays ; Hydrogen Bonding ; Models, Molecular ; mutants ; Mutation ; Phylogeny ; sequence analysis ; Substrate Specificity ; transaminase ; transaminases ; Transaminases - chemistry ; Transaminases - metabolism</subject><ispartof>The FEBS journal, 2015-07, Vol.282 (13), p.2512-2526</ispartof><rights>2015 FEBS</rights><rights>2015 FEBS.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3743-fb28138f94987c141ce0c9645bd4f7c06b48dc14520a7436c708dfbfd8ff80423</citedby><cites>FETCH-LOGICAL-c3743-fb28138f94987c141ce0c9645bd4f7c06b48dc14520a7436c708dfbfd8ff80423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ffebs.13293$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ffebs.13293$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25846556$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deszcz, Dawid</creatorcontrib><creatorcontrib>Affaticati, Pierre</creatorcontrib><creatorcontrib>Ladkau, Nadine</creatorcontrib><creatorcontrib>Gegel, Alex</creatorcontrib><creatorcontrib>Ward, John M</creatorcontrib><creatorcontrib>Hailes, Helen C</creatorcontrib><creatorcontrib>Dalby, Paul A</creatorcontrib><title>Single active‐site mutants are sufficient to enhance serine:pyruvate α‐transaminase activity in an ω‐transaminase</title><title>The FEBS journal</title><addtitle>FEBS J</addtitle><description>We have analyzed the natural evolution of transaminase structure and sequence between an α‐transaminase serine‐pyruvate aminotransferase and an ω‐transaminase from Chromobacterium violaceum with < 20% sequence identity, and identified the active‐site regions that are least conserved structurally. We also show that these structural changes correlate strongly with transaminase substrate specificity during evolution and therefore might normally be presumed to be essential determinants of substrate specificity. However, key residues are often conserved spatially during evolution and yet originate from within a different region of the sequence via structural reorganizations. In the present study, we also show that α‐transaminase‐type serine‐pyruvate aminotransferase activity can be engineered into the CV2025 ω‐transaminase scaffold with any one of many possible single‐point mutations at three key positions, without the requirement for significant backbone remodeling, or repositioning of the residue from a different region of sequence. This finding has significant implications for enzyme redesign in which solutions to substrate specificity changes may be found more efficiently than is achieved by engineering in all sequence and structure determinants identified by correlation to substrate specificity.</description><subject>active sites</subject><subject>aminotransferase</subject><subject>biocatalysis</subject><subject>Catalytic Domain</subject><subject>Chromobacterium violaceum</subject><subject>directed evolution</subject><subject>engineering</subject><subject>evolution</subject><subject>High-Throughput Screening Assays</subject><subject>Hydrogen Bonding</subject><subject>Models, Molecular</subject><subject>mutants</subject><subject>Mutation</subject><subject>Phylogeny</subject><subject>sequence analysis</subject><subject>Substrate Specificity</subject><subject>transaminase</subject><subject>transaminases</subject><subject>Transaminases - chemistry</subject><subject>Transaminases - metabolism</subject><issn>1742-464X</issn><issn>1742-4658</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1OHDEURq0oKJBNGh6AuERIu9hjz4wnHSD-JCSKDVI6y-O5BqMZz8b2EE2Xli6vwovAO_AkeNmFIkVuc6-uzneKD6FtSmY0zb6BOswoyyr2AW3RkmdTXuTi4_vNf26izyHcEsJyXlWf0GaWi4TkxRYa59Zdt4CVjvYOnv_8DTYC7oaoXAxYecBhMMZqCy7i2GNwN8rp9AVvHXxfjH64Uynx-JCy0SsXVGedCmujjSO2DiuHn-7_Ab6gDaPaAF_Xe4KuTo5_HJ1NLy5Pz48OLqaalZxNTZ0JyoSpeCVKTTnVQHRV8LxuuCk1KWoumvTPM6ISX-iSiMbUphHGCMIzNkG7K-_C978GCFF2NmhoW-WgH4KkRcWoSPElurdCte9D8GDkwttO-VFSIpdVy2XV8rXqBO-svUPdQfOOvnWbALoCftsWxv-o5Mnx4fxN-m2VMaqX6trbIK_mGaEFISQjpKLsBceAmNM</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Deszcz, Dawid</creator><creator>Affaticati, Pierre</creator><creator>Ladkau, Nadine</creator><creator>Gegel, Alex</creator><creator>Ward, John M</creator><creator>Hailes, Helen C</creator><creator>Dalby, Paul A</creator><general>Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201507</creationdate><title>Single active‐site mutants are sufficient to enhance serine:pyruvate α‐transaminase activity in an ω‐transaminase</title><author>Deszcz, Dawid ; Affaticati, Pierre ; Ladkau, Nadine ; Gegel, Alex ; Ward, John M ; Hailes, Helen C ; Dalby, Paul A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3743-fb28138f94987c141ce0c9645bd4f7c06b48dc14520a7436c708dfbfd8ff80423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>active sites</topic><topic>aminotransferase</topic><topic>biocatalysis</topic><topic>Catalytic Domain</topic><topic>Chromobacterium violaceum</topic><topic>directed evolution</topic><topic>engineering</topic><topic>evolution</topic><topic>High-Throughput Screening Assays</topic><topic>Hydrogen Bonding</topic><topic>Models, Molecular</topic><topic>mutants</topic><topic>Mutation</topic><topic>Phylogeny</topic><topic>sequence analysis</topic><topic>Substrate Specificity</topic><topic>transaminase</topic><topic>transaminases</topic><topic>Transaminases - chemistry</topic><topic>Transaminases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deszcz, Dawid</creatorcontrib><creatorcontrib>Affaticati, Pierre</creatorcontrib><creatorcontrib>Ladkau, Nadine</creatorcontrib><creatorcontrib>Gegel, Alex</creatorcontrib><creatorcontrib>Ward, John M</creatorcontrib><creatorcontrib>Hailes, Helen C</creatorcontrib><creatorcontrib>Dalby, Paul A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The FEBS journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deszcz, Dawid</au><au>Affaticati, Pierre</au><au>Ladkau, Nadine</au><au>Gegel, Alex</au><au>Ward, John M</au><au>Hailes, Helen C</au><au>Dalby, Paul A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single active‐site mutants are sufficient to enhance serine:pyruvate α‐transaminase activity in an ω‐transaminase</atitle><jtitle>The FEBS journal</jtitle><addtitle>FEBS J</addtitle><date>2015-07</date><risdate>2015</risdate><volume>282</volume><issue>13</issue><spage>2512</spage><epage>2526</epage><pages>2512-2526</pages><issn>1742-464X</issn><eissn>1742-4658</eissn><abstract>We have analyzed the natural evolution of transaminase structure and sequence between an α‐transaminase serine‐pyruvate aminotransferase and an ω‐transaminase from Chromobacterium violaceum with < 20% sequence identity, and identified the active‐site regions that are least conserved structurally. We also show that these structural changes correlate strongly with transaminase substrate specificity during evolution and therefore might normally be presumed to be essential determinants of substrate specificity. However, key residues are often conserved spatially during evolution and yet originate from within a different region of the sequence via structural reorganizations. In the present study, we also show that α‐transaminase‐type serine‐pyruvate aminotransferase activity can be engineered into the CV2025 ω‐transaminase scaffold with any one of many possible single‐point mutations at three key positions, without the requirement for significant backbone remodeling, or repositioning of the residue from a different region of sequence. This finding has significant implications for enzyme redesign in which solutions to substrate specificity changes may be found more efficiently than is achieved by engineering in all sequence and structure determinants identified by correlation to substrate specificity.</abstract><cop>England</cop><pub>Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies</pub><pmid>25846556</pmid><doi>10.1111/febs.13293</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | active sites aminotransferase biocatalysis Catalytic Domain Chromobacterium violaceum directed evolution engineering evolution High-Throughput Screening Assays Hydrogen Bonding Models, Molecular mutants Mutation Phylogeny sequence analysis Substrate Specificity transaminase transaminases Transaminases - chemistry Transaminases - metabolism |
| title | Single active‐site mutants are sufficient to enhance serine:pyruvate α‐transaminase activity in an ω‐transaminase |
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