rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress
OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing P SARK ::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in P SARK ::IPT...
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| Veröffentlicht in: | Plant molecular biology 2015-07, Vol.88 (4-5), p.401-413 |
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| creator | Raineri, Jesica Wang, Songhu Peleg, Zvi Blumwald, Eduardo Chan, Raquel Lia |
| description | OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing P SARK ::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in P SARK ::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant. |
| doi_str_mv | 10.1007/s11103-015-0329-7 |
| format | Article |
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A transcriptomic analysis of the drought response of transgenic rice plants expressing P SARK ::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in P SARK ::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/s11103-015-0329-7</identifier><identifier>PMID: 25957211</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Base Sequence ; Binding Sites - genetics ; Biochemistry ; Bioinformatics ; Biomedical and Life Sciences ; calmodulin ; Calmodulin-Binding Proteins - genetics ; Calmodulin-Binding Proteins - metabolism ; DNA, Plant - genetics ; DNA, Plant - metabolism ; Drought ; Droughts ; Gene Knockout Techniques ; gene overexpression ; genes ; Genes, Plant ; knockout mutants ; Life Sciences ; Molecular Sequence Data ; Oryza - genetics ; Oryza - growth & development ; Oryza - metabolism ; Oryza sativa ; Plant biology ; Plant Pathology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Sciences ; Plants, Genetically Modified ; Promoter Regions, Genetic ; quantitative polymerase chain reaction ; rice ; Site selection ; Stress, Physiological ; systematic evolution of ligands by exponential enrichment ; transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcriptome ; transcriptomics ; transgenic plants ; Water deficit ; water stress</subject><ispartof>Plant molecular biology, 2015-07, Vol.88 (4-5), p.401-413</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-61926e18b33829979c0b0b4379f0fdef66e363ba39040046921bd91457cdc15d3</citedby><cites>FETCH-LOGICAL-c466t-61926e18b33829979c0b0b4379f0fdef66e363ba39040046921bd91457cdc15d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11103-015-0329-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11103-015-0329-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25957211$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raineri, Jesica</creatorcontrib><creatorcontrib>Wang, Songhu</creatorcontrib><creatorcontrib>Peleg, Zvi</creatorcontrib><creatorcontrib>Blumwald, Eduardo</creatorcontrib><creatorcontrib>Chan, Raquel Lia</creatorcontrib><title>rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><addtitle>Plant Mol Biol</addtitle><description>OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing P SARK ::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in P SARK ::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant.</description><subject>Base Sequence</subject><subject>Binding Sites - genetics</subject><subject>Biochemistry</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>calmodulin</subject><subject>Calmodulin-Binding Proteins - genetics</subject><subject>Calmodulin-Binding Proteins - metabolism</subject><subject>DNA, Plant - genetics</subject><subject>DNA, Plant - metabolism</subject><subject>Drought</subject><subject>Droughts</subject><subject>Gene Knockout Techniques</subject><subject>gene overexpression</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>knockout mutants</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>Oryza - genetics</subject><subject>Oryza - growth & development</subject><subject>Oryza - metabolism</subject><subject>Oryza sativa</subject><subject>Plant biology</subject><subject>Plant Pathology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic</subject><subject>quantitative polymerase chain reaction</subject><subject>rice</subject><subject>Site selection</subject><subject>Stress, Physiological</subject><subject>systematic evolution of ligands by exponential enrichment</subject><subject>transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcriptome</subject><subject>transcriptomics</subject><subject>transgenic plants</subject><subject>Water deficit</subject><subject>water stress</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kU1rFTEUhoMo7e3HD3CjgW7cjM1JZpKbpZTWioVCPxBXIZPJXFPunYw5GaX_vhmmirjoKpDzvE8OeQl5C-wjMKZOEQCYqBg0FRNcV-oVWUGjRNUwvn5NVgykquoa-D45QHxgrKSE3CP7vNGN4gAr0qbgPM3JDuhSGHOIA-2tyzHRa_x28_V7rWhAaukYMeTwy9PkN9PWzkDsaf4xX-AYByyWSH_b7BPtfB9cyBRzmeERedPbLfrj5_OQ3F-c351dVlfXn7-cfbqqXC1lriRoLj2sWyHWXGulHWtZWwule9YXo5ReSNFaoVnNWC01h7bTUDfKdQ6aThySD4t3TPHn5DGbXUDnt1s7-DihgRJRDQiAgp78hz7EKQ1lu5kqZqHlTMFCuRQRk-_NmMLOpkcDzMwFmKUAUwowcwFGlcy7Z_PU7nz3N_HnxwvAFwDLaNj49M_TL1jfL6HeRmM3KaC5v-Wl3lIpV0oJ8QSdm5gT</recordid><startdate>20150701</startdate><enddate>20150701</enddate><creator>Raineri, Jesica</creator><creator>Wang, Songhu</creator><creator>Peleg, Zvi</creator><creator>Blumwald, Eduardo</creator><creator>Chan, Raquel Lia</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20150701</creationdate><title>rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress</title><author>Raineri, Jesica ; Wang, Songhu ; Peleg, Zvi ; Blumwald, Eduardo ; Chan, Raquel Lia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-61926e18b33829979c0b0b4379f0fdef66e363ba39040046921bd91457cdc15d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Base Sequence</topic><topic>Binding Sites - genetics</topic><topic>Biochemistry</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>calmodulin</topic><topic>Calmodulin-Binding Proteins - genetics</topic><topic>Calmodulin-Binding Proteins - metabolism</topic><topic>DNA, Plant - genetics</topic><topic>DNA, Plant - metabolism</topic><topic>Drought</topic><topic>Droughts</topic><topic>Gene Knockout Techniques</topic><topic>gene overexpression</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>knockout mutants</topic><topic>Life Sciences</topic><topic>Molecular Sequence Data</topic><topic>Oryza - genetics</topic><topic>Oryza - growth & development</topic><topic>Oryza - metabolism</topic><topic>Oryza sativa</topic><topic>Plant biology</topic><topic>Plant Pathology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Sciences</topic><topic>Plants, Genetically Modified</topic><topic>Promoter Regions, Genetic</topic><topic>quantitative polymerase chain reaction</topic><topic>rice</topic><topic>Site selection</topic><topic>Stress, Physiological</topic><topic>systematic evolution of ligands by exponential enrichment</topic><topic>transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcriptome</topic><topic>transcriptomics</topic><topic>transgenic plants</topic><topic>Water deficit</topic><topic>water stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raineri, Jesica</creatorcontrib><creatorcontrib>Wang, Songhu</creatorcontrib><creatorcontrib>Peleg, Zvi</creatorcontrib><creatorcontrib>Blumwald, Eduardo</creatorcontrib><creatorcontrib>Chan, Raquel Lia</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raineri, Jesica</au><au>Wang, Songhu</au><au>Peleg, Zvi</au><au>Blumwald, Eduardo</au><au>Chan, Raquel Lia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress</atitle><jtitle>Plant molecular biology</jtitle><stitle>Plant Mol Biol</stitle><addtitle>Plant Mol Biol</addtitle><date>2015-07-01</date><risdate>2015</risdate><volume>88</volume><issue>4-5</issue><spage>401</spage><epage>413</epage><pages>401-413</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing P SARK ::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in P SARK ::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>25957211</pmid><doi>10.1007/s11103-015-0329-7</doi><tpages>13</tpages></addata></record> |
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| subjects | Base Sequence Binding Sites - genetics Biochemistry Bioinformatics Biomedical and Life Sciences calmodulin Calmodulin-Binding Proteins - genetics Calmodulin-Binding Proteins - metabolism DNA, Plant - genetics DNA, Plant - metabolism Drought Droughts Gene Knockout Techniques gene overexpression genes Genes, Plant knockout mutants Life Sciences Molecular Sequence Data Oryza - genetics Oryza - growth & development Oryza - metabolism Oryza sativa Plant biology Plant Pathology Plant Proteins - genetics Plant Proteins - metabolism Plant Sciences Plants, Genetically Modified Promoter Regions, Genetic quantitative polymerase chain reaction rice Site selection Stress, Physiological systematic evolution of ligands by exponential enrichment transcription factors Transcription Factors - genetics Transcription Factors - metabolism Transcriptome transcriptomics transgenic plants Water deficit water stress |
| title | rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress |
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