Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor
Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A des...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2014-09, Vol.86 (17), p.8680-8686 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8686 |
|---|---|
| container_issue | 17 |
| container_start_page | 8680 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 86 |
| creator | Kovář, David Farka, Zdeněk Skládal, Petr |
| description | Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator in the chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 × 104 CFU·L–1 of air. The total time from sample collection to detection was 16 min. The system was fully automated and controlled remotely through a local network. |
| doi_str_mv | 10.1021/ac501623m |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1692384974</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1692384974</sourcerecordid><originalsourceid>FETCH-LOGICAL-a442t-3fe1bc88c33dc55670307e3d03909d41f7b9d4160e7f44edd71c4edf3129f0dd3</originalsourceid><addsrcrecordid>eNqF0btOwzAUBmALgWgpDLwAioSQYAgcX3IbS7lVqlQGOkepfVxcJXGJk4E-Pa5aKgQD01k-_0c-PyHnFG4pMHpXyAhozHh1QPo0YhDGacoOSR8AeMgSgB45cW4JQKl3x6THIkjThEZ9Mn3AFmVrbB1YHQyxsc6WZo0quDe2tAsjizIYLrBuXTBzpl4E7TsGrwbXFkv_sDEyGFdVV1uHtbPNKTnSRenwbDcHZPb0-DZ6CSfT5_FoOAkLIVgbco10LtNUcq5kFMUJcEiQK-AZZEpQncw3IwZMtBCoVEKlH5pTlmlQig_I9TZ31diPDl2bV8ZJLMuiRtu5nMYZ46nIEvE_jaIsZpD6Yw3I5S-6tF1T-494FVMquBAbdbNV0l_LNajzVWOqovnMKeSbQvJ9Id5e7BK7eYVqL78b8OBqCwrpfmz7E_QFsJ-QMg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1561143440</pqid></control><display><type>article</type><title>Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor</title><source>MEDLINE</source><source>ACS Publications</source><creator>Kovář, David ; Farka, Zdeněk ; Skládal, Petr</creator><creatorcontrib>Kovář, David ; Farka, Zdeněk ; Skládal, Petr</creatorcontrib><description>Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator in the chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 × 104 CFU·L–1 of air. The total time from sample collection to detection was 16 min. The system was fully automated and controlled remotely through a local network.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac501623m</identifier><identifier>PMID: 25088715</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Aerosols ; Aerosols - analysis ; Air Microbiology ; Antibodies - immunology ; Bacteria ; Bioaerosols ; Biological ; Biosensing Techniques - methods ; Biosensors ; Chambers ; Escherichia coli - isolation & purification ; Immunoglobulins ; Immunosensors ; Microorganisms ; Piezoelectricity ; Quartz Crystal Microbalance Techniques ; Respiratory diseases ; Toxicology ; Toxins</subject><ispartof>Analytical chemistry (Washington), 2014-09, Vol.86 (17), p.8680-8686</ispartof><rights>Copyright © 2014 American Chemical Society</rights><rights>Copyright American Chemical Society Sep 2, 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a442t-3fe1bc88c33dc55670307e3d03909d41f7b9d4160e7f44edd71c4edf3129f0dd3</citedby><cites>FETCH-LOGICAL-a442t-3fe1bc88c33dc55670307e3d03909d41f7b9d4160e7f44edd71c4edf3129f0dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac501623m$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac501623m$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27074,27922,27923,56736,56786</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25088715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kovář, David</creatorcontrib><creatorcontrib>Farka, Zdeněk</creatorcontrib><creatorcontrib>Skládal, Petr</creatorcontrib><title>Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator in the chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 × 104 CFU·L–1 of air. The total time from sample collection to detection was 16 min. The system was fully automated and controlled remotely through a local network.</description><subject>Aerosols</subject><subject>Aerosols - analysis</subject><subject>Air Microbiology</subject><subject>Antibodies - immunology</subject><subject>Bacteria</subject><subject>Bioaerosols</subject><subject>Biological</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Chambers</subject><subject>Escherichia coli - isolation & purification</subject><subject>Immunoglobulins</subject><subject>Immunosensors</subject><subject>Microorganisms</subject><subject>Piezoelectricity</subject><subject>Quartz Crystal Microbalance Techniques</subject><subject>Respiratory diseases</subject><subject>Toxicology</subject><subject>Toxins</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0btOwzAUBmALgWgpDLwAioSQYAgcX3IbS7lVqlQGOkepfVxcJXGJk4E-Pa5aKgQD01k-_0c-PyHnFG4pMHpXyAhozHh1QPo0YhDGacoOSR8AeMgSgB45cW4JQKl3x6THIkjThEZ9Mn3AFmVrbB1YHQyxsc6WZo0quDe2tAsjizIYLrBuXTBzpl4E7TsGrwbXFkv_sDEyGFdVV1uHtbPNKTnSRenwbDcHZPb0-DZ6CSfT5_FoOAkLIVgbco10LtNUcq5kFMUJcEiQK-AZZEpQncw3IwZMtBCoVEKlH5pTlmlQig_I9TZ31diPDl2bV8ZJLMuiRtu5nMYZ46nIEvE_jaIsZpD6Yw3I5S-6tF1T-494FVMquBAbdbNV0l_LNajzVWOqovnMKeSbQvJ9Id5e7BK7eYVqL78b8OBqCwrpfmz7E_QFsJ-QMg</recordid><startdate>20140902</startdate><enddate>20140902</enddate><creator>Kovář, David</creator><creator>Farka, Zdeněk</creator><creator>Skládal, Petr</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20140902</creationdate><title>Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor</title><author>Kovář, David ; Farka, Zdeněk ; Skládal, Petr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a442t-3fe1bc88c33dc55670307e3d03909d41f7b9d4160e7f44edd71c4edf3129f0dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aerosols</topic><topic>Aerosols - analysis</topic><topic>Air Microbiology</topic><topic>Antibodies - immunology</topic><topic>Bacteria</topic><topic>Bioaerosols</topic><topic>Biological</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Chambers</topic><topic>Escherichia coli - isolation & purification</topic><topic>Immunoglobulins</topic><topic>Immunosensors</topic><topic>Microorganisms</topic><topic>Piezoelectricity</topic><topic>Quartz Crystal Microbalance Techniques</topic><topic>Respiratory diseases</topic><topic>Toxicology</topic><topic>Toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kovář, David</creatorcontrib><creatorcontrib>Farka, Zdeněk</creatorcontrib><creatorcontrib>Skládal, Petr</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kovář, David</au><au>Farka, Zdeněk</au><au>Skládal, Petr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2014-09-02</date><risdate>2014</risdate><volume>86</volume><issue>17</issue><spage>8680</spage><epage>8686</epage><pages>8680-8686</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator in the chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 × 104 CFU·L–1 of air. The total time from sample collection to detection was 16 min. The system was fully automated and controlled remotely through a local network.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>25088715</pmid><doi>10.1021/ac501623m</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2014-09, Vol.86 (17), p.8680-8686 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1692384974 |
| source | MEDLINE; ACS Publications |
| subjects | Aerosols Aerosols - analysis Air Microbiology Antibodies - immunology Bacteria Bioaerosols Biological Biosensing Techniques - methods Biosensors Chambers Escherichia coli - isolation & purification Immunoglobulins Immunosensors Microorganisms Piezoelectricity Quartz Crystal Microbalance Techniques Respiratory diseases Toxicology Toxins |
| title | Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T22%3A14%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20Aerosolized%20Biological%20Agents%20Using%20the%20Piezoelectric%20Immunosensor&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Kova%CC%81r%CC%8C,%20David&rft.date=2014-09-02&rft.volume=86&rft.issue=17&rft.spage=8680&rft.epage=8686&rft.pages=8680-8686&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac501623m&rft_dat=%3Cproquest_cross%3E1692384974%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1561143440&rft_id=info:pmid/25088715&rfr_iscdi=true |