Isolation of copper oxide (CuO) nanoparticles resistant Pseudomonas strains from soil and investigation on possible mechanism for resistance
The present study deals with isolation and characterization of copper oxide nanoparticles resistant Pseudomonas strains that were isolated from the soil collected from mining and refining sites of Sarcheshmeh copper mine in the Kerman Province of Iran. The three isolates were selected based on high...
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| Veröffentlicht in: | World journal of microbiology & biotechnology 2014-03, Vol.30 (3), p.809-817 |
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| creator | Soltani Nezhad, Shahla Rabbani Khorasgani, Mohammad Emtiazi, Giti Yaghoobi, Mohammad Mehdi Shakeri, Shahryar |
| description | The present study deals with isolation and characterization of copper oxide nanoparticles resistant
Pseudomonas
strains that were isolated from the soil collected from mining and refining sites of Sarcheshmeh copper mine in the Kerman Province of Iran. The three isolates were selected based on high level of copper oxide nanoparticles (CuO NPs) resistance. The isolates were authentically identified as
Pseudomonas
fluorescens
CuO-1,
Pseudomonas fluorescens
CuO-2 and
Pseudomonas
sp. CuO-3 by morphological, biochemical and 16S rDNA gene sequencing analysis. The growth pattern of these isolates with all the studied CuO NPs concentrations was similar to that of control (without CuO NPs) indicating that CuO NPs would not affect the growth of isolated strains. A reduction in the amount of exopolysaccharides was observed after CuO NPs—
P. fluorescens
CuO-1 culture supernatant interaction. The Fourier transform infrared spectroscopy (FT-IR) peaks for the exopolysaccharides extracted from the bacterial culture supernatant and the interacted CuO NPs were almost similar. The exopolysaccharide capping of the CuO NPs was confirmed by FT-IR and X-ray diffraction analysis. The study of bacterial exopolysaccharides capped CuO NPs with
E. coli
PTCC 1338 and
S. aureus
PTCC 1113 showed less toxicity compared to uncoated CuO NPs. Our study suggests that the capping of nanoparticles by bacterially produced exopolysaccharides serve as the probable mechanism of tolerance. |
| doi_str_mv | 10.1007/s11274-013-1481-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1692379483</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3210298081</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-ae86376e3e129651e1f9a7e87bb8641fdcb242759711b73f0b5fca5eb55295b23</originalsourceid><addsrcrecordid>eNqFkctu1TAQhi0EoofCA7BBltiURcDjSxwv0RGXSpXKAtaWk0yKq8QOnqSCd-ChydE5VAgJsfJivvk8Mz9jz0G8BiHsGwKQVlcCVAW6gUo9YDswVlXCWfmQ7YQzrlLOqTP2hOhWiK3LqcfsTGrQtRJ2x35eUh7DEnPieeBdnmcsPH-PPfKL_Xr9iqeQ8hzKErsRiRekSEtIC_9EuPZ5yikQp6WEmIgPJU-cchx5SD2P6Q5piTcne-JzJortiHzC7mtIkSY-5HLv7PApezSEkfDZ6T1nX96_-7z_WF1df7jcv72qOm3NUgVsamVrVAjS1QYQBhcsNrZtm1rD0Het1NIaZwFaqwbRmqELBltjpDOtVOfs4uidS_62bkP6KVKH4xgS5pU81E4q63Sj_o8aYZRswJoNffkXepvXkrZFPGhXiwOmNwqOVFe2cxQc_FziFMoPD8IfUvXHVP2Wqj-k6g9DvDiZ13bC_r7jd4wbII8AbaV0g-WPr_9p_QXFGK5q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1496032814</pqid></control><display><type>article</type><title>Isolation of copper oxide (CuO) nanoparticles resistant Pseudomonas strains from soil and investigation on possible mechanism for resistance</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Soltani Nezhad, Shahla ; Rabbani Khorasgani, Mohammad ; Emtiazi, Giti ; Yaghoobi, Mohammad Mehdi ; Shakeri, Shahryar</creator><creatorcontrib>Soltani Nezhad, Shahla ; Rabbani Khorasgani, Mohammad ; Emtiazi, Giti ; Yaghoobi, Mohammad Mehdi ; Shakeri, Shahryar</creatorcontrib><description>The present study deals with isolation and characterization of copper oxide nanoparticles resistant
Pseudomonas
strains that were isolated from the soil collected from mining and refining sites of Sarcheshmeh copper mine in the Kerman Province of Iran. The three isolates were selected based on high level of copper oxide nanoparticles (CuO NPs) resistance. The isolates were authentically identified as
Pseudomonas
fluorescens
CuO-1,
Pseudomonas fluorescens
CuO-2 and
Pseudomonas
sp. CuO-3 by morphological, biochemical and 16S rDNA gene sequencing analysis. The growth pattern of these isolates with all the studied CuO NPs concentrations was similar to that of control (without CuO NPs) indicating that CuO NPs would not affect the growth of isolated strains. A reduction in the amount of exopolysaccharides was observed after CuO NPs—
P. fluorescens
CuO-1 culture supernatant interaction. The Fourier transform infrared spectroscopy (FT-IR) peaks for the exopolysaccharides extracted from the bacterial culture supernatant and the interacted CuO NPs were almost similar. The exopolysaccharide capping of the CuO NPs was confirmed by FT-IR and X-ray diffraction analysis. The study of bacterial exopolysaccharides capped CuO NPs with
E. coli
PTCC 1338 and
S. aureus
PTCC 1113 showed less toxicity compared to uncoated CuO NPs. Our study suggests that the capping of nanoparticles by bacterially produced exopolysaccharides serve as the probable mechanism of tolerance.</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-013-1481-3</identifier><identifier>PMID: 24146307</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Analysis ; Anti-Infective Agents - metabolism ; Antibiotics ; Applied Microbiology ; Bacteria ; Bacterial Typing Techniques ; Biochemistry ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Capping ; Copper ; Copper - metabolism ; COPPER OXIDE ; Copper oxides ; COPPER RESOURCES ; Culture ; CUPRIC OXIDE ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Drug Resistance, Bacterial ; E coli ; Environmental Engineering/Biotechnology ; Escherichia coli ; Fourier transforms ; Infrared spectroscopy ; Iran ; Life Sciences ; Microbiology ; Molecular Sequence Data ; Nanoparticles ; Nanoparticles - metabolism ; Original Paper ; OXIDES ; PARTICLES ; Phylogenetics ; Physical properties ; Polysaccharides, Bacterial - metabolism ; Pseudomonas ; Pseudomonas - drug effects ; Pseudomonas - genetics ; Pseudomonas - isolation & purification ; Pseudomonas - physiology ; Pseudomonas fluorescens ; REFINING ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Soil Microbiology ; SOILS ; Spectroscopy, Fourier Transform Infrared ; Staphylococcus aureus ; STRAIN ; Studies ; X-Ray Diffraction</subject><ispartof>World journal of microbiology & biotechnology, 2014-03, Vol.30 (3), p.809-817</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-ae86376e3e129651e1f9a7e87bb8641fdcb242759711b73f0b5fca5eb55295b23</citedby><cites>FETCH-LOGICAL-c475t-ae86376e3e129651e1f9a7e87bb8641fdcb242759711b73f0b5fca5eb55295b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11274-013-1481-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11274-013-1481-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24146307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soltani Nezhad, Shahla</creatorcontrib><creatorcontrib>Rabbani Khorasgani, Mohammad</creatorcontrib><creatorcontrib>Emtiazi, Giti</creatorcontrib><creatorcontrib>Yaghoobi, Mohammad Mehdi</creatorcontrib><creatorcontrib>Shakeri, Shahryar</creatorcontrib><title>Isolation of copper oxide (CuO) nanoparticles resistant Pseudomonas strains from soil and investigation on possible mechanism for resistance</title><title>World journal of microbiology & biotechnology</title><addtitle>World J Microbiol Biotechnol</addtitle><addtitle>World J Microbiol Biotechnol</addtitle><description>The present study deals with isolation and characterization of copper oxide nanoparticles resistant
Pseudomonas
strains that were isolated from the soil collected from mining and refining sites of Sarcheshmeh copper mine in the Kerman Province of Iran. The three isolates were selected based on high level of copper oxide nanoparticles (CuO NPs) resistance. The isolates were authentically identified as
Pseudomonas
fluorescens
CuO-1,
Pseudomonas fluorescens
CuO-2 and
Pseudomonas
sp. CuO-3 by morphological, biochemical and 16S rDNA gene sequencing analysis. The growth pattern of these isolates with all the studied CuO NPs concentrations was similar to that of control (without CuO NPs) indicating that CuO NPs would not affect the growth of isolated strains. A reduction in the amount of exopolysaccharides was observed after CuO NPs—
P. fluorescens
CuO-1 culture supernatant interaction. The Fourier transform infrared spectroscopy (FT-IR) peaks for the exopolysaccharides extracted from the bacterial culture supernatant and the interacted CuO NPs were almost similar. The exopolysaccharide capping of the CuO NPs was confirmed by FT-IR and X-ray diffraction analysis. The study of bacterial exopolysaccharides capped CuO NPs with
E. coli
PTCC 1338 and
S. aureus
PTCC 1113 showed less toxicity compared to uncoated CuO NPs. Our study suggests that the capping of nanoparticles by bacterially produced exopolysaccharides serve as the probable mechanism of tolerance.</description><subject>Analysis</subject><subject>Anti-Infective Agents - metabolism</subject><subject>Antibiotics</subject><subject>Applied Microbiology</subject><subject>Bacteria</subject><subject>Bacterial Typing Techniques</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Capping</subject><subject>Copper</subject><subject>Copper - metabolism</subject><subject>COPPER OXIDE</subject><subject>Copper oxides</subject><subject>COPPER RESOURCES</subject><subject>Culture</subject><subject>CUPRIC OXIDE</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Drug Resistance, Bacterial</subject><subject>E coli</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Escherichia coli</subject><subject>Fourier transforms</subject><subject>Infrared spectroscopy</subject><subject>Iran</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nanoparticles</subject><subject>Nanoparticles - metabolism</subject><subject>Original Paper</subject><subject>OXIDES</subject><subject>PARTICLES</subject><subject>Phylogenetics</subject><subject>Physical properties</subject><subject>Polysaccharides, Bacterial - metabolism</subject><subject>Pseudomonas</subject><subject>Pseudomonas - drug effects</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - isolation & purification</subject><subject>Pseudomonas - physiology</subject><subject>Pseudomonas fluorescens</subject><subject>REFINING</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Soil Microbiology</subject><subject>SOILS</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Staphylococcus aureus</subject><subject>STRAIN</subject><subject>Studies</subject><subject>X-Ray 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of copper oxide (CuO) nanoparticles resistant Pseudomonas strains from soil and investigation on possible mechanism for resistance</title><author>Soltani Nezhad, Shahla ; Rabbani Khorasgani, Mohammad ; Emtiazi, Giti ; Yaghoobi, Mohammad Mehdi ; Shakeri, Shahryar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-ae86376e3e129651e1f9a7e87bb8641fdcb242759711b73f0b5fca5eb55295b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analysis</topic><topic>Anti-Infective Agents - metabolism</topic><topic>Antibiotics</topic><topic>Applied Microbiology</topic><topic>Bacteria</topic><topic>Bacterial Typing Techniques</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Capping</topic><topic>Copper</topic><topic>Copper - metabolism</topic><topic>COPPER OXIDE</topic><topic>Copper oxides</topic><topic>COPPER RESOURCES</topic><topic>Culture</topic><topic>CUPRIC OXIDE</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Drug Resistance, Bacterial</topic><topic>E coli</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Escherichia coli</topic><topic>Fourier transforms</topic><topic>Infrared spectroscopy</topic><topic>Iran</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nanoparticles</topic><topic>Nanoparticles - metabolism</topic><topic>Original Paper</topic><topic>OXIDES</topic><topic>PARTICLES</topic><topic>Phylogenetics</topic><topic>Physical properties</topic><topic>Polysaccharides, Bacterial - metabolism</topic><topic>Pseudomonas</topic><topic>Pseudomonas - drug effects</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - isolation & 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Database</collection><jtitle>World journal of microbiology & biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soltani Nezhad, Shahla</au><au>Rabbani Khorasgani, Mohammad</au><au>Emtiazi, Giti</au><au>Yaghoobi, Mohammad Mehdi</au><au>Shakeri, Shahryar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of copper oxide (CuO) nanoparticles resistant Pseudomonas strains from soil and investigation on possible mechanism for resistance</atitle><jtitle>World journal of microbiology & biotechnology</jtitle><stitle>World J Microbiol Biotechnol</stitle><addtitle>World J Microbiol Biotechnol</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>30</volume><issue>3</issue><spage>809</spage><epage>817</epage><pages>809-817</pages><issn>0959-3993</issn><eissn>1573-0972</eissn><abstract>The present study deals with isolation and characterization of copper oxide nanoparticles resistant
Pseudomonas
strains that were isolated from the soil collected from mining and refining sites of Sarcheshmeh copper mine in the Kerman Province of Iran. The three isolates were selected based on high level of copper oxide nanoparticles (CuO NPs) resistance. The isolates were authentically identified as
Pseudomonas
fluorescens
CuO-1,
Pseudomonas fluorescens
CuO-2 and
Pseudomonas
sp. CuO-3 by morphological, biochemical and 16S rDNA gene sequencing analysis. The growth pattern of these isolates with all the studied CuO NPs concentrations was similar to that of control (without CuO NPs) indicating that CuO NPs would not affect the growth of isolated strains. A reduction in the amount of exopolysaccharides was observed after CuO NPs—
P. fluorescens
CuO-1 culture supernatant interaction. The Fourier transform infrared spectroscopy (FT-IR) peaks for the exopolysaccharides extracted from the bacterial culture supernatant and the interacted CuO NPs were almost similar. The exopolysaccharide capping of the CuO NPs was confirmed by FT-IR and X-ray diffraction analysis. The study of bacterial exopolysaccharides capped CuO NPs with
E. coli
PTCC 1338 and
S. aureus
PTCC 1113 showed less toxicity compared to uncoated CuO NPs. Our study suggests that the capping of nanoparticles by bacterially produced exopolysaccharides serve as the probable mechanism of tolerance.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24146307</pmid><doi>10.1007/s11274-013-1481-3</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0959-3993 |
| ispartof | World journal of microbiology & biotechnology, 2014-03, Vol.30 (3), p.809-817 |
| issn | 0959-3993 1573-0972 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1692379483 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Analysis Anti-Infective Agents - metabolism Antibiotics Applied Microbiology Bacteria Bacterial Typing Techniques Biochemistry Biomedical and Life Sciences Biosynthesis Biotechnology Capping Copper Copper - metabolism COPPER OXIDE Copper oxides COPPER RESOURCES Culture CUPRIC OXIDE DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Drug Resistance, Bacterial E coli Environmental Engineering/Biotechnology Escherichia coli Fourier transforms Infrared spectroscopy Iran Life Sciences Microbiology Molecular Sequence Data Nanoparticles Nanoparticles - metabolism Original Paper OXIDES PARTICLES Phylogenetics Physical properties Polysaccharides, Bacterial - metabolism Pseudomonas Pseudomonas - drug effects Pseudomonas - genetics Pseudomonas - isolation & purification Pseudomonas - physiology Pseudomonas fluorescens REFINING RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Soil Microbiology SOILS Spectroscopy, Fourier Transform Infrared Staphylococcus aureus STRAIN Studies X-Ray Diffraction |
| title | Isolation of copper oxide (CuO) nanoparticles resistant Pseudomonas strains from soil and investigation on possible mechanism for resistance |
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