Glycine Oxidase Based High-Throughput Solid-Phase Assay for Substrate Profiling and Directed Evolution of (R)- and (S)‑Selective Amine Transaminases

Transaminases represent one of the most important enzymes of the biocatalytic toolbox for chiral amine synthesis as they allow asymmetric synthesis with quantitative yields and high enantioselectivity. In order to enable substrate profiling of transaminases for acceptance of different amines, a glyc...

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Veröffentlicht in:Analytical chemistry (Washington) 2014-12, Vol.86 (23), p.11847-11853
Hauptverfasser: Weiß, Martin S., Pavlidis, Ioannis V., Vickers, Clare, Höhne, Matthias, Bornscheuer, Uwe T.
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Sprache:eng
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