Sensitive Detection of DNA Methyltransferase Activity Based on Exonuclease-Mediated Target Recycling

DNA methylation plays vital roles in various biological processes in both prokaryotes and eukaryotes. In bacteria, modification of adenine at N6 can protect bacterial DNA against cleavage by restriction enzymes, and bacterial DNA adenine methyltransferases are essential for bacterial virulence and v...

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Veröffentlicht in:	Analytical chemistry (Washington) 2014-11, Vol.86 (22), p.11269-11274
Hauptverfasser:	Xing, Xi-Wen, Tang, Feng, Wu, Jun, Chu, Jie-Mei, Feng, Yu-Qi, Zhou, Xiang, Yuan, Bi-Feng
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenines Assaying Bacteria Cells, Cultured Deoxyribonucleic acid DNA methylation E coli Enzyme Activation - drug effects Enzymes Escherichia coli Escherichia coli - cytology Escherichia coli - enzymology Eukaryotes Exonucleases - metabolism Inhibitors Methylation Prokaryotes Recycling Site-Specific DNA-Methyltransferase (Adenine-Specific) - antagonists & inhibitors Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism Strategy Structure-Activity Relationship
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