Real-Time NMR Monitoring of Protein-Folding Kinetics by a Recycle Flow System for Temperature Jump
An NMR method was developed that allows for real-time monitoring of reactions (on the order of seconds) induced by a temperature jump. In a recycle flow system, heating and cooling baths were integrated, with the latter inside the NMR probe. A refolding reaction of ribonuclease A was triggered by ra...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2013-10, Vol.85 (20), p.9439-9443 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Yamasaki, Kazuhiko Obara, Yuji Hasegawa, Manabu Tanaka, Hideki Yamasaki, Tomoko Wakuda, Tsuyoshi Okada, Michiya Kohzuma, Takamitsu |
| description | An NMR method was developed that allows for real-time monitoring of reactions (on the order of seconds) induced by a temperature jump. In a recycle flow system, heating and cooling baths were integrated, with the latter inside the NMR probe. A refolding reaction of ribonuclease A was triggered by rapid cooling and monitored by a series of NMR measurements over 12 s. Data were processed by principal component analysis, in which a factor related to the structural change with an exponential rate constant of 0.2–0.7 s–1 was successfully separated from factors related to baseline instability and/or noise. Temperature dependency of the rate constant revealed the entropy-driven formation of the transition state of the refolding reaction. |
| doi_str_mv | 10.1021/ac401579e |
| format | Article |
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In a recycle flow system, heating and cooling baths were integrated, with the latter inside the NMR probe. A refolding reaction of ribonuclease A was triggered by rapid cooling and monitored by a series of NMR measurements over 12 s. Data were processed by principal component analysis, in which a factor related to the structural change with an exponential rate constant of 0.2–0.7 s–1 was successfully separated from factors related to baseline instability and/or noise. 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Chem</addtitle><description>An NMR method was developed that allows for real-time monitoring of reactions (on the order of seconds) induced by a temperature jump. In a recycle flow system, heating and cooling baths were integrated, with the latter inside the NMR probe. A refolding reaction of ribonuclease A was triggered by rapid cooling and monitored by a series of NMR measurements over 12 s. Data were processed by principal component analysis, in which a factor related to the structural change with an exponential rate constant of 0.2–0.7 s–1 was successfully separated from factors related to baseline instability and/or noise. Temperature dependency of the rate constant revealed the entropy-driven formation of the transition state of the refolding reaction.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cooling systems</subject><subject>Entropy</subject><subject>Heating</subject><subject>Instability</subject><subject>Kinetics</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>Monitoring</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Principal Component Analysis</subject><subject>Principal components analysis</subject><subject>Protein Folding</subject><subject>Rate constants</subject><subject>Reaction kinetics</subject><subject>Real time</subject><subject>Ribonuclease, Pancreatic - chemistry</subject><subject>Symbols</subject><subject>Temperature</subject><subject>Temperature effects</subject><subject>Time Factors</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1LxDAQBuAgiq4fB_-ABETQQ3XysWl6FHH9XJV1PZc0nUqlbdakRfbfW91VRA-eBoaHdxheQnYZHDPg7MRYCWwYJ7hCBmzIIVJa81UyAAAR8Rhgg2yG8ALAGDC1Tja4BK6lEAOSTdBU0bSskd6NJ3TsmrJ1vmyeqSvog3ctlk00clX-sbopG2xLG2g2p4ZO0M5thXRUuTf6OA8t1rRwnk6xnqE3beeRXnf1bJusFaYKuLOcW-RpdD49u4xu7y-uzk5vIyNi1UbK5AiJ4FbaWEIGWotEQ65itEMDSnJpjbSSZ0JKAZBkUhecCxOrXEpexGKLHC5yZ969dhjatC6DxaoyDboupEwlXMih7h__l_YnJNM6SXq6_4u-uM43_SOfSitIGO_V0UJZ70LwWKQzX9bGz1MG6UdH6XdHvd1bJnZZjfm3_CqlBwcLYGz4ce1P0Dv725SN</recordid><startdate>20131015</startdate><enddate>20131015</enddate><creator>Yamasaki, Kazuhiko</creator><creator>Obara, Yuji</creator><creator>Hasegawa, Manabu</creator><creator>Tanaka, Hideki</creator><creator>Yamasaki, Tomoko</creator><creator>Wakuda, Tsuyoshi</creator><creator>Okada, Michiya</creator><creator>Kohzuma, Takamitsu</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20131015</creationdate><title>Real-Time NMR Monitoring of Protein-Folding Kinetics by a Recycle Flow System for Temperature Jump</title><author>Yamasaki, Kazuhiko ; 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| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Cattle Cooling systems Entropy Heating Instability Kinetics Magnetic Resonance Spectroscopy - methods Monitoring NMR Nuclear magnetic resonance Principal Component Analysis Principal components analysis Protein Folding Rate constants Reaction kinetics Real time Ribonuclease, Pancreatic - chemistry Symbols Temperature Temperature effects Time Factors |
| title | Real-Time NMR Monitoring of Protein-Folding Kinetics by a Recycle Flow System for Temperature Jump |
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