Optimization of Gold Nanoparticle Biosynthesis by Escherichia coli DH5α and its Conjugation with Gentamicin
Metal nanoparticles are one option for targeted drug delivery. In order to increase antibiotic efficiency and decrease its side effects, antibiotic conjugated nanoparticles have been known as a suitable approach. The aim of this study was optimization of gold nanoparticle biosynthesis by Escherichia...
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| Veröffentlicht in: | Journal of nano research 2015-05, Vol.32, p.93-105 |
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| creator | Akbarzadeh Khiavi, Azim Motamedi, Hossein Khademi Mazdeh, Samaneh Mehrabi, Mohammad Reza |
| description | Metal nanoparticles are one option for targeted drug delivery. In order to increase antibiotic efficiency and decrease its side effects, antibiotic conjugated nanoparticles have been known as a suitable approach. The aim of this study was optimization of gold nanoparticle biosynthesis by Escherichia coli DH5α and its conjugation with gentamicin. For this purpose gold nanoparticles were biosynthesized from HAuCl4 and confirmed by Uv/ Vis, XRD, DLS and SEM. Then the effects of different parameters on optimum conditions for gold nanoparticles production were investigated. The MIC and MBC of gentamicin and its conjugate were investigated against E. coli, Clostridium perfringens and Clostridium botulinum. The results revealed that among different treatments, centrifuge (10000 rpm, 10 min) and sonication are the optimum conditions for gold nanoparticle production with less than 10 nm sizes. Filtration was also the best method for purifying nanoparticles. The conjugated nanoparticles significantly reduced the MIC of gentamicin against E. coli and also overcame the natural resistance of tested anaerobic bacteria. In conclusion, the optimized method is an effective, inexpensive and environmental friendly method for biosynthesis of gold nanoparticles. Overcoming natural resistance of anaerobic bacteria using antibiotic conjugates with nanoparticles provides hopes for further experiments and in vivo studies. |
| doi_str_mv | 10.4028/www.scientific.net/JNanoR.32.93 |
| format | Article |
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In order to increase antibiotic efficiency and decrease its side effects, antibiotic conjugated nanoparticles have been known as a suitable approach. The aim of this study was optimization of gold nanoparticle biosynthesis by Escherichia coli DH5α and its conjugation with gentamicin. For this purpose gold nanoparticles were biosynthesized from HAuCl4 and confirmed by Uv/ Vis, XRD, DLS and SEM. Then the effects of different parameters on optimum conditions for gold nanoparticles production were investigated. The MIC and MBC of gentamicin and its conjugate were investigated against E. coli, Clostridium perfringens and Clostridium botulinum. The results revealed that among different treatments, centrifuge (10000 rpm, 10 min) and sonication are the optimum conditions for gold nanoparticle production with less than 10 nm sizes. Filtration was also the best method for purifying nanoparticles. The conjugated nanoparticles significantly reduced the MIC of gentamicin against E. coli and also overcame the natural resistance of tested anaerobic bacteria. In conclusion, the optimized method is an effective, inexpensive and environmental friendly method for biosynthesis of gold nanoparticles. 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In order to increase antibiotic efficiency and decrease its side effects, antibiotic conjugated nanoparticles have been known as a suitable approach. The aim of this study was optimization of gold nanoparticle biosynthesis by Escherichia coli DH5α and its conjugation with gentamicin. For this purpose gold nanoparticles were biosynthesized from HAuCl4 and confirmed by Uv/ Vis, XRD, DLS and SEM. Then the effects of different parameters on optimum conditions for gold nanoparticles production were investigated. The MIC and MBC of gentamicin and its conjugate were investigated against E. coli, Clostridium perfringens and Clostridium botulinum. The results revealed that among different treatments, centrifuge (10000 rpm, 10 min) and sonication are the optimum conditions for gold nanoparticle production with less than 10 nm sizes. Filtration was also the best method for purifying nanoparticles. The conjugated nanoparticles significantly reduced the MIC of gentamicin against E. coli and also overcame the natural resistance of tested anaerobic bacteria. In conclusion, the optimized method is an effective, inexpensive and environmental friendly method for biosynthesis of gold nanoparticles. Overcoming natural resistance of anaerobic bacteria using antibiotic conjugates with nanoparticles provides hopes for further experiments and in vivo studies.</description><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Biosynthesis</subject><subject>Clostridium botulinum</subject><subject>Clostridium perfringens</subject><subject>Conjugation</subject><subject>Escherichia coli</subject><subject>Gold</subject><subject>Nanoparticles</subject><subject>Nanostructure</subject><subject>Optimization</subject><issn>1662-5250</issn><issn>1661-9897</issn><issn>1661-9897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkctu1DAUhiMEEqXwDt7BJqkv8SUrVIYyBVVUqsracs445Iwy9mB7NBreqi_SZyIlSGy7Omfx69N_zldV7xltWsrNxfF4bDKgDwUHhCb4cvHtuwvxrhG86cSL6owpxerOdPrl353Xkkv6unqT85ZSJZnqzqrpdl9wh79dwRhIHMg6ThvyxNm7VBAmTz5hzKdQRp8xk_5ErjKMPiGM6AjECcnna_n4QFzYECyZrGLYHn4uvCOWkaznim6HgOFt9WpwU_bv_s3z6seXq_vVdX1zu_66urypQXBdaiGHnjJKwTjQfKM3bTeAlkwbp7jynnHpAbyTPThGGbTtwHVvWsN973yvxHn1YeHuU_x18LnYHWbw0-SCj4ds58u5EKqT7TOiRivNjTFz9OMShRRzTn6w-4Q7l06WUfukxM5K7H8ldlZiFyVWcNuJmXC5EEpyIRcPo93GQwrzL57N-AObZ6Jx</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Akbarzadeh Khiavi, Azim</creator><creator>Motamedi, Hossein</creator><creator>Khademi Mazdeh, Samaneh</creator><creator>Mehrabi, Mohammad Reza</creator><general>Trans Tech Publications Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20150501</creationdate><title>Optimization of Gold Nanoparticle Biosynthesis by Escherichia coli DH5α and its Conjugation with Gentamicin</title><author>Akbarzadeh Khiavi, Azim ; Motamedi, Hossein ; Khademi Mazdeh, Samaneh ; Mehrabi, Mohammad Reza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-35fb0100c8ac72d7d49fc75178a626ee125eccea5bca101c44f27b8482ebaeb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Biosynthesis</topic><topic>Clostridium botulinum</topic><topic>Clostridium perfringens</topic><topic>Conjugation</topic><topic>Escherichia coli</topic><topic>Gold</topic><topic>Nanoparticles</topic><topic>Nanostructure</topic><topic>Optimization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akbarzadeh Khiavi, Azim</creatorcontrib><creatorcontrib>Motamedi, Hossein</creatorcontrib><creatorcontrib>Khademi Mazdeh, Samaneh</creatorcontrib><creatorcontrib>Mehrabi, Mohammad Reza</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of nano research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akbarzadeh Khiavi, Azim</au><au>Motamedi, Hossein</au><au>Khademi Mazdeh, Samaneh</au><au>Mehrabi, Mohammad Reza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of Gold Nanoparticle Biosynthesis by Escherichia coli DH5α and its Conjugation with Gentamicin</atitle><jtitle>Journal of nano research</jtitle><date>2015-05-01</date><risdate>2015</risdate><volume>32</volume><spage>93</spage><epage>105</epage><pages>93-105</pages><issn>1662-5250</issn><issn>1661-9897</issn><eissn>1661-9897</eissn><abstract>Metal nanoparticles are one option for targeted drug delivery. In order to increase antibiotic efficiency and decrease its side effects, antibiotic conjugated nanoparticles have been known as a suitable approach. The aim of this study was optimization of gold nanoparticle biosynthesis by Escherichia coli DH5α and its conjugation with gentamicin. For this purpose gold nanoparticles were biosynthesized from HAuCl4 and confirmed by Uv/ Vis, XRD, DLS and SEM. Then the effects of different parameters on optimum conditions for gold nanoparticles production were investigated. The MIC and MBC of gentamicin and its conjugate were investigated against E. coli, Clostridium perfringens and Clostridium botulinum. The results revealed that among different treatments, centrifuge (10000 rpm, 10 min) and sonication are the optimum conditions for gold nanoparticle production with less than 10 nm sizes. Filtration was also the best method for purifying nanoparticles. The conjugated nanoparticles significantly reduced the MIC of gentamicin against E. coli and also overcame the natural resistance of tested anaerobic bacteria. In conclusion, the optimized method is an effective, inexpensive and environmental friendly method for biosynthesis of gold nanoparticles. Overcoming natural resistance of anaerobic bacteria using antibiotic conjugates with nanoparticles provides hopes for further experiments and in vivo studies.</abstract><pub>Trans Tech Publications Ltd</pub><doi>10.4028/www.scientific.net/JNanoR.32.93</doi><tpages>13</tpages></addata></record> |
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| subjects | Antibiotics Bacteria Biosynthesis Clostridium botulinum Clostridium perfringens Conjugation Escherichia coli Gold Nanoparticles Nanostructure Optimization |
| title | Optimization of Gold Nanoparticle Biosynthesis by Escherichia coli DH5α and its Conjugation with Gentamicin |
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