Bacterial Autoinducer‑2 Detection via an Engineered Quorum Sensing Protein

Autoinducer-2 (AI-2) is a Quorum Sensing (QS) molecule utilized by bacteria in interspecies communication. More recently, it is identified to be vital in regulating QS pathways in a number of human and foodborne pathogens. Methods to detect AI-2 in a rapid and highly sensitive manner can help in the...

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Veröffentlicht in:	Analytical chemistry (Washington) 2015-03, Vol.87 (5), p.2608-2614
Hauptverfasser:	Raut, Nilesh, Joel, Smita, Pasini, Patrizia, Daunert, Sylvia
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Bacteria Bacterial infections Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Biosensing Techniques - methods Blood Coumarins - chemistry Detection Energy transfer Fluorescence Fluorescence Resonance Energy Transfer - methods Fretting Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Healthy Volunteers Homoserine - analogs & derivatives Homoserine - analysis Human Humans Lactones - analysis Molecules Pathogens Protein Conformation Protein Engineering - methods Proteins Quorum Sensing Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Saliva - chemistry
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