Using Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures (NECEEM) for Simultaneous Determination of Concentration and Equilibrium Constant

Nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) is a versatile tool for studying affinity binding. Here we describe a NECEEM-based approach for simultaneous determination of both the equilibrium constant, K d, and the unknown concentration of a binder that we call a target,...

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Veröffentlicht in:	Analytical chemistry (Washington) 2015-03, Vol.87 (5), p.3099-3106
Hauptverfasser:	Kanoatov, Mirzo, Galievsky, Victor A, Krylova, Svetlana M, Cherney, Leonid T, Jankowski, Hanna K, Krylov, Sergey N
Format:	Artikel
Sprache:	eng
Schlagworte:	Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - metabolism Bacterial Proteins - chemistry Bacterial Proteins - metabolism Binders Binding sites Capillarity Capillary electrophoresis Chemical equilibrium Computer Simulation Dilution DNA-Binding Proteins - metabolism Electrophoresis Electrophoresis, Capillary - instrumentation Electrophoresis, Capillary - methods Equilibrium Escherichia coli Proteins - chemistry Escherichia coli Proteins - metabolism Kinetics Mathematical analysis Mathematical models Molecules MutS DNA Mismatch-Binding Protein - chemistry MutS DNA Mismatch-Binding Protein - metabolism
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