Expression of melatonin receptors genes and genes associated with regulation of their activity in endometrial cancer
The aim of the study was to evaluate transcription activity of melatonin receptors and genes associated with regulation of their activity in endometrial adenocarcinoma to identify probable diagnostic and prognostic molecular markers. The material included endometrial adenocarcinoma tissue samples of...
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| Veröffentlicht in: | Ginekologia polska 2015-01, Vol.86 (4), p.248-255 |
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| creator | Witek, Andrzej Jęda, Agnieszka Baliś, Michał Orchel, Joanna Sikora, Bartosz Skubis, Aleksandra Szota-Czyż, Justyna Janikowski, Tomasz Mazurek, Urszula |
| description | The aim of the study was to evaluate transcription activity of melatonin receptors and genes associated with regulation of their activity in endometrial adenocarcinoma to identify probable diagnostic and prognostic molecular markers.
The material included endometrial adenocarcinoma tissue samples of histopathological grades G1, G2, G3, and normal endometrium. The molecular analysis was performed on 37 patient samples. Total RNA was extracted and used for the microarray HG-U133A analysis. Among 22 283 ID mRNA, only entities of genes associated with regulation of melatonin receptors activity were selected. qRT-PCR was employed for validation, what allowed to compare melatonin receptor genes activation in endometrial cancer tissues to the normal endometrium.
The results of the microarray experiments showed that only 18 ID mRNA were differential in endometrial cancer samples as compared to the control at p-value1.5. These genes were identified as differentially expressed in grade G2-ASMTL, GNA 11, PER2, PTGDS and in grade G3-GNA12, GNA 11. Silencing of RGS4 encoding RGP4, which regulates signal transmission by G protein, was observed in all cancer groups, independently of the histopathological grade.
The profile expression of genes associated with regulation of melatonin receptors activity was different and dependent on the histopathological grade of endometrial cancer and can be an additional diagnostic and prognostic marker Statistically significant was the down-regulation of melatonin biosynthesis genes (ASMTL) and melatonin signal transmitters (GNA 11, GNA 12, RTGS). |
| doi_str_mv | 10.17772/gp/2069 |
| format | Article |
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The material included endometrial adenocarcinoma tissue samples of histopathological grades G1, G2, G3, and normal endometrium. The molecular analysis was performed on 37 patient samples. Total RNA was extracted and used for the microarray HG-U133A analysis. Among 22 283 ID mRNA, only entities of genes associated with regulation of melatonin receptors activity were selected. qRT-PCR was employed for validation, what allowed to compare melatonin receptor genes activation in endometrial cancer tissues to the normal endometrium.
The results of the microarray experiments showed that only 18 ID mRNA were differential in endometrial cancer samples as compared to the control at p-value<0.05 and FC(log2)>1.5. These genes were identified as differentially expressed in grade G2-ASMTL, GNA 11, PER2, PTGDS and in grade G3-GNA12, GNA 11. Silencing of RGS4 encoding RGP4, which regulates signal transmission by G protein, was observed in all cancer groups, independently of the histopathological grade.
The profile expression of genes associated with regulation of melatonin receptors activity was different and dependent on the histopathological grade of endometrial cancer and can be an additional diagnostic and prognostic marker Statistically significant was the down-regulation of melatonin biosynthesis genes (ASMTL) and melatonin signal transmitters (GNA 11, GNA 12, RTGS).</description><identifier>ISSN: 0017-0011</identifier><identifier>EISSN: 2543-6767</identifier><identifier>DOI: 10.17772/gp/2069</identifier><identifier>PMID: 26117982</identifier><language>eng ; pol</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Carcinoma, Endometrioid - genetics ; Carcinoma, Endometrioid - metabolism ; Down-Regulation ; Endometrial cancer ; Endometrial Neoplasms - genetics ; Endometrial Neoplasms - metabolism ; Endometrium ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Melatonin ; Real-Time Polymerase Chain Reaction ; Receptors, Melatonin - genetics ; Receptors, Melatonin - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism</subject><ispartof>Ginekologia polska, 2015-01, Vol.86 (4), p.248-255</ispartof><rights>2015. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c275t-4a0b2861062114322a623523f58b9261d4a4fe0c0e896a824ee55200c09285ab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26117982$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Witek, Andrzej</creatorcontrib><creatorcontrib>Jęda, Agnieszka</creatorcontrib><creatorcontrib>Baliś, Michał</creatorcontrib><creatorcontrib>Orchel, Joanna</creatorcontrib><creatorcontrib>Sikora, Bartosz</creatorcontrib><creatorcontrib>Skubis, Aleksandra</creatorcontrib><creatorcontrib>Szota-Czyż, Justyna</creatorcontrib><creatorcontrib>Janikowski, Tomasz</creatorcontrib><creatorcontrib>Mazurek, Urszula</creatorcontrib><title>Expression of melatonin receptors genes and genes associated with regulation of their activity in endometrial cancer</title><title>Ginekologia polska</title><addtitle>Ginekol Pol</addtitle><description>The aim of the study was to evaluate transcription activity of melatonin receptors and genes associated with regulation of their activity in endometrial adenocarcinoma to identify probable diagnostic and prognostic molecular markers.
The material included endometrial adenocarcinoma tissue samples of histopathological grades G1, G2, G3, and normal endometrium. The molecular analysis was performed on 37 patient samples. Total RNA was extracted and used for the microarray HG-U133A analysis. Among 22 283 ID mRNA, only entities of genes associated with regulation of melatonin receptors activity were selected. qRT-PCR was employed for validation, what allowed to compare melatonin receptor genes activation in endometrial cancer tissues to the normal endometrium.
The results of the microarray experiments showed that only 18 ID mRNA were differential in endometrial cancer samples as compared to the control at p-value<0.05 and FC(log2)>1.5. These genes were identified as differentially expressed in grade G2-ASMTL, GNA 11, PER2, PTGDS and in grade G3-GNA12, GNA 11. Silencing of RGS4 encoding RGP4, which regulates signal transmission by G protein, was observed in all cancer groups, independently of the histopathological grade.
The profile expression of genes associated with regulation of melatonin receptors activity was different and dependent on the histopathological grade of endometrial cancer and can be an additional diagnostic and prognostic marker Statistically significant was the down-regulation of melatonin biosynthesis genes (ASMTL) and melatonin signal transmitters (GNA 11, GNA 12, RTGS).</description><subject>Carcinoma, Endometrioid - genetics</subject><subject>Carcinoma, Endometrioid - metabolism</subject><subject>Down-Regulation</subject><subject>Endometrial cancer</subject><subject>Endometrial Neoplasms - genetics</subject><subject>Endometrial Neoplasms - metabolism</subject><subject>Endometrium</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Melatonin</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptors, Melatonin - genetics</subject><subject>Receptors, Melatonin - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><issn>0017-0011</issn><issn>2543-6767</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkcFq3DAQQEVpyC6bQL4gCHrpxY00liXrWEKSFgK5NGejlccbLbbkSHLb_fsq6aaFzEEziKfHjIaQC86-cKUUXO3mK2BSfyBraERdSSXVR7JmjKuqHHxFzlPasxISFGh9SlYgOVe6hTXJN7_niCm54GkY6ISjycE7TyNanHOIie7QY6LG929VSsE6k7Gnv1x-KuRuKa-OhvyELlJjs_vp8oEWE_o-TJijMyO1xluMZ-RkMGPC82PekMfbmx_X36r7h7vv11_vKwuqyZUwbAut5KVvzkUNYCTUDdRD0251GaEXRgzILMNWS9OCQGwaYOVCQ9uYbb0hn_965xieF0y5m1yyOI7GY1hSx6UG0KLhUNBP79B9WKIv3XUgpADOlGj_C20MKUUcujm6ycRDx1n3uoxuN3cvyyjo5VG4bCfs_4FvX1__AZhgg-I</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Witek, Andrzej</creator><creator>Jęda, Agnieszka</creator><creator>Baliś, Michał</creator><creator>Orchel, Joanna</creator><creator>Sikora, Bartosz</creator><creator>Skubis, Aleksandra</creator><creator>Szota-Czyż, Justyna</creator><creator>Janikowski, Tomasz</creator><creator>Mazurek, Urszula</creator><general>Wydawnictwo Via Medica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20150101</creationdate><title>Expression of melatonin receptors genes and genes associated with regulation of their activity in endometrial cancer</title><author>Witek, Andrzej ; Jęda, Agnieszka ; Baliś, Michał ; Orchel, Joanna ; Sikora, Bartosz ; Skubis, Aleksandra ; Szota-Czyż, Justyna ; Janikowski, Tomasz ; Mazurek, Urszula</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-4a0b2861062114322a623523f58b9261d4a4fe0c0e896a824ee55200c09285ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; pol</language><creationdate>2015</creationdate><topic>Carcinoma, Endometrioid - genetics</topic><topic>Carcinoma, Endometrioid - metabolism</topic><topic>Down-Regulation</topic><topic>Endometrial cancer</topic><topic>Endometrial Neoplasms - genetics</topic><topic>Endometrial Neoplasms - metabolism</topic><topic>Endometrium</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Melatonin</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptors, Melatonin - genetics</topic><topic>Receptors, Melatonin - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Witek, Andrzej</creatorcontrib><creatorcontrib>Jęda, Agnieszka</creatorcontrib><creatorcontrib>Baliś, Michał</creatorcontrib><creatorcontrib>Orchel, Joanna</creatorcontrib><creatorcontrib>Sikora, Bartosz</creatorcontrib><creatorcontrib>Skubis, Aleksandra</creatorcontrib><creatorcontrib>Szota-Czyż, Justyna</creatorcontrib><creatorcontrib>Janikowski, Tomasz</creatorcontrib><creatorcontrib>Mazurek, Urszula</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Ginekologia polska</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Witek, Andrzej</au><au>Jęda, Agnieszka</au><au>Baliś, Michał</au><au>Orchel, Joanna</au><au>Sikora, Bartosz</au><au>Skubis, Aleksandra</au><au>Szota-Czyż, Justyna</au><au>Janikowski, Tomasz</au><au>Mazurek, Urszula</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of melatonin receptors genes and genes associated with regulation of their activity in endometrial cancer</atitle><jtitle>Ginekologia polska</jtitle><addtitle>Ginekol Pol</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>86</volume><issue>4</issue><spage>248</spage><epage>255</epage><pages>248-255</pages><issn>0017-0011</issn><eissn>2543-6767</eissn><abstract>The aim of the study was to evaluate transcription activity of melatonin receptors and genes associated with regulation of their activity in endometrial adenocarcinoma to identify probable diagnostic and prognostic molecular markers.
The material included endometrial adenocarcinoma tissue samples of histopathological grades G1, G2, G3, and normal endometrium. The molecular analysis was performed on 37 patient samples. Total RNA was extracted and used for the microarray HG-U133A analysis. Among 22 283 ID mRNA, only entities of genes associated with regulation of melatonin receptors activity were selected. qRT-PCR was employed for validation, what allowed to compare melatonin receptor genes activation in endometrial cancer tissues to the normal endometrium.
The results of the microarray experiments showed that only 18 ID mRNA were differential in endometrial cancer samples as compared to the control at p-value<0.05 and FC(log2)>1.5. These genes were identified as differentially expressed in grade G2-ASMTL, GNA 11, PER2, PTGDS and in grade G3-GNA12, GNA 11. Silencing of RGS4 encoding RGP4, which regulates signal transmission by G protein, was observed in all cancer groups, independently of the histopathological grade.
The profile expression of genes associated with regulation of melatonin receptors activity was different and dependent on the histopathological grade of endometrial cancer and can be an additional diagnostic and prognostic marker Statistically significant was the down-regulation of melatonin biosynthesis genes (ASMTL) and melatonin signal transmitters (GNA 11, GNA 12, RTGS).</abstract><cop>Poland</cop><pub>Wydawnictwo Via Medica</pub><pmid>26117982</pmid><doi>10.17772/gp/2069</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Carcinoma, Endometrioid - genetics Carcinoma, Endometrioid - metabolism Down-Regulation Endometrial cancer Endometrial Neoplasms - genetics Endometrial Neoplasms - metabolism Endometrium Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Melatonin Real-Time Polymerase Chain Reaction Receptors, Melatonin - genetics Receptors, Melatonin - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism |
| title | Expression of melatonin receptors genes and genes associated with regulation of their activity in endometrial cancer |
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