Diversity of Frankia nodule endophytes of the actinorhizal shrub Ceanothus as assessed by RFLP patterns from single nodule lobes
Root nodules of the actinorhizal shrub Ceanothus were collected from seven sites from its native range. DNA was extracted from individual nodule lobes using a cetyl trimethyl ammonium bromide (CTAB) extraction procedure. Three DNA probes were used in combination with two restriction endonucleases to...
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| Veröffentlicht in: | Soil biology & biochemistry 1994, Vol.26 (5), p.547-552 |
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| creator | Baker, Dwight D. Mullin, Beth C. |
| description | Root nodules of the actinorhizal shrub
Ceanothus were collected from seven sites from its native range. DNA was extracted from individual nodule lobes using a cetyl trimethyl ammonium bromide (CTAB) extraction procedure. Three DNA probes were used in combination with two restriction endonucleases to evaluate the extent of restriction fragment length polymorphism (RFLP) diversity within and among populations of
Ceanothus endophytes. We observed some diversity using a
nif DH gene probe; however there was no correlation of RFLP pattern and geographic site. Using two random
Frankia probes, we observed more diversity among the
Ceanothus endophytes than with the
nif probe. Differences in RFLP patterns were observed among plants at a single geographic site and between geographical sites. The results demonstrated that considerable diversity exists among
Frankia strains symbiotic with
Ceanothus, as has been shown for pure-cultured
Frankia strains isolated from other actinorhizal genera. We have also demonstrated the usefulness of this method for the study of
Frankia ecology
in planta. |
| doi_str_mv | 10.1016/0038-0717(94)90241-0 |
| format | Article |
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Ceanothus were collected from seven sites from its native range. DNA was extracted from individual nodule lobes using a cetyl trimethyl ammonium bromide (CTAB) extraction procedure. Three DNA probes were used in combination with two restriction endonucleases to evaluate the extent of restriction fragment length polymorphism (RFLP) diversity within and among populations of
Ceanothus endophytes. We observed some diversity using a
nif DH gene probe; however there was no correlation of RFLP pattern and geographic site. Using two random
Frankia probes, we observed more diversity among the
Ceanothus endophytes than with the
nif probe. Differences in RFLP patterns were observed among plants at a single geographic site and between geographical sites. The results demonstrated that considerable diversity exists among
Frankia strains symbiotic with
Ceanothus, as has been shown for pure-cultured
Frankia strains isolated from other actinorhizal genera. We have also demonstrated the usefulness of this method for the study of
Frankia ecology
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Ceanothus were collected from seven sites from its native range. DNA was extracted from individual nodule lobes using a cetyl trimethyl ammonium bromide (CTAB) extraction procedure. Three DNA probes were used in combination with two restriction endonucleases to evaluate the extent of restriction fragment length polymorphism (RFLP) diversity within and among populations of
Ceanothus endophytes. We observed some diversity using a
nif DH gene probe; however there was no correlation of RFLP pattern and geographic site. Using two random
Frankia probes, we observed more diversity among the
Ceanothus endophytes than with the
nif probe. Differences in RFLP patterns were observed among plants at a single geographic site and between geographical sites. The results demonstrated that considerable diversity exists among
Frankia strains symbiotic with
Ceanothus, as has been shown for pure-cultured
Frankia strains isolated from other actinorhizal genera. We have also demonstrated the usefulness of this method for the study of
Frankia ecology
in planta.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biochemistry and biology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Ceanothus</subject><subject>Chemical, physicochemical, biochemical and biological properties</subject><subject>Frankia</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Physics, chemistry, biochemistry and biology of agricultural and forest soils</subject><subject>Soil science</subject><issn>0038-0717</issn><issn>1879-3428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNp9kE2LFDEQhhtRcFz9Bx5yENFDa766O7kIMuvsCgOK6Dmkk2o72tMZU-mF8eRPN-0MexQKcqin3qo8VfWc0TeMsvYtpULVtGPdKy1fa8olq-mDasNUp2shuXpYbe6Rx9UTxB-UUt4wsan-XIc7SBjyicSB7JKdfwZL5uiXCQjMPh7HUwZcm3kEYl0Oc0xj-G0ngmNaerIFO8c8LkjsWgilPOlP5Mtu_5kcbc6QZiRDigeCYf5eci_xU-wBn1aPBjshPLu8V9W33Yev29t6_-nm4_b9vnaibXPtlWsa13Rtbz10TKi20S33wLhrlGD9wJXnUgMbCsIbxVvBu6azwjvdStmLq-rlOfeY4q8FMJtDQAfTZGeICxrWaqa0YAWUZ9CliJhgMMcUDjadDKNm1W1Wl2Z1abQ0_3QbWsZeXPItOjsNxaQLeD8rmSxn6oK9O2NQ_noXIBl0AWYHPiRw2fgY_r_nL7_hlLU</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Baker, Dwight D.</creator><creator>Mullin, Beth C.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SN</scope><scope>C1K</scope></search><sort><creationdate>1994</creationdate><title>Diversity of Frankia nodule endophytes of the actinorhizal shrub Ceanothus as assessed by RFLP patterns from single nodule lobes</title><author>Baker, Dwight D. ; Mullin, Beth C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-d8c55c576bade713865962de12c5831bf28d249e1f76b2582632757a3dc9644b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biochemistry and biology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Ceanothus</topic><topic>Chemical, physicochemical, biochemical and biological properties</topic><topic>Frankia</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Physics, chemistry, biochemistry and biology of agricultural and forest soils</topic><topic>Soil science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baker, Dwight D.</creatorcontrib><creatorcontrib>Mullin, Beth C.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Soil biology & biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baker, Dwight D.</au><au>Mullin, Beth C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diversity of Frankia nodule endophytes of the actinorhizal shrub Ceanothus as assessed by RFLP patterns from single nodule lobes</atitle><jtitle>Soil biology & biochemistry</jtitle><date>1994</date><risdate>1994</risdate><volume>26</volume><issue>5</issue><spage>547</spage><epage>552</epage><pages>547-552</pages><issn>0038-0717</issn><eissn>1879-3428</eissn><coden>SBIOAH</coden><abstract>Root nodules of the actinorhizal shrub
Ceanothus were collected from seven sites from its native range. DNA was extracted from individual nodule lobes using a cetyl trimethyl ammonium bromide (CTAB) extraction procedure. Three DNA probes were used in combination with two restriction endonucleases to evaluate the extent of restriction fragment length polymorphism (RFLP) diversity within and among populations of
Ceanothus endophytes. We observed some diversity using a
nif DH gene probe; however there was no correlation of RFLP pattern and geographic site. Using two random
Frankia probes, we observed more diversity among the
Ceanothus endophytes than with the
nif probe. Differences in RFLP patterns were observed among plants at a single geographic site and between geographical sites. The results demonstrated that considerable diversity exists among
Frankia strains symbiotic with
Ceanothus, as has been shown for pure-cultured
Frankia strains isolated from other actinorhizal genera. We have also demonstrated the usefulness of this method for the study of
Frankia ecology
in planta.</abstract><cop>Oxford</cop><cop>New York, NY</cop><pub>Elsevier Ltd</pub><doi>10.1016/0038-0717(94)90241-0</doi><tpages>6</tpages></addata></record> |
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| ispartof | Soil biology & biochemistry, 1994, Vol.26 (5), p.547-552 |
| issn | 0038-0717 1879-3428 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Agronomy. Soil science and plant productions Biochemistry and biology Biological and medical sciences Biotechnology Ceanothus Chemical, physicochemical, biochemical and biological properties Frankia Fundamental and applied biological sciences. Psychology Microbiology Physics, chemistry, biochemistry and biology of agricultural and forest soils Soil science |
| title | Diversity of Frankia nodule endophytes of the actinorhizal shrub Ceanothus as assessed by RFLP patterns from single nodule lobes |
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