Molecular analysis of pentachlorophenol degradation
A limited number of microorganisms have been described for their ability to partially degrade pentachlorophenol (PCP), or to completely mineralize it. Several years ago we chose one of these microorganisms, Flavobacterium sp. strain ATCC 39723, for use in a detailed molecular analysis of the catabol...
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| Veröffentlicht in: | Biodegradation (Dordrecht) 1994-12, Vol.5 (3/4), p.277-288 |
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| container_title | Biodegradation (Dordrecht) |
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| creator | Orser, C.S Lange, C.C |
| description | A limited number of microorganisms have been described for their ability to partially degrade pentachlorophenol (PCP), or to completely mineralize it. Several years ago we chose one of these microorganisms, Flavobacterium sp. strain ATCC 39723, for use in a detailed molecular analysis of the catabolism of PCP. This strain was chosen because it had previously been studied in great detail for its growth characteristics in relation to degradation of PCP. In this paper we provide an overview of the degradation pathway of PCP to 2,6-dichloro-p-hydroquinone by Flavobacterium. The specific biochemical reactions and the genes encoding the enzymes are reviewed. The successful transformation and site specific mutagenesis of Flavobacterium, as well as the discovery of two new pcp alleles is also presented. |
| doi_str_mv | 10.1007/BF00696465 |
| format | Article |
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The successful transformation and site specific mutagenesis of Flavobacterium, as well as the discovery of two new pcp alleles is also presented.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>biochemical pathways</subject><subject>biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>enzyme activity</subject><subject>Flavobacterium</subject><subject>Flavobacterium - enzymology</subject><subject>Flavobacterium - genetics</subject><subject>Flavobacterium - metabolism</subject><subject>Fundamental and applied biological sciences. 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| ispartof | Biodegradation (Dordrecht), 1994-12, Vol.5 (3/4), p.277-288 |
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| language | eng |
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| subjects | Amino Acid Sequence amino acid sequences biochemical pathways biodegradation Biodegradation, Environmental Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology enzyme activity Flavobacterium Flavobacterium - enzymology Flavobacterium - genetics Flavobacterium - metabolism Fundamental and applied biological sciences. Psychology genes Genes, Bacterial Genetics Glutathione Transferase - genetics Glutathione Transferase - metabolism metabolism Mission oriented research Molecular Sequence Data pentachlorophenol Pentachlorophenol - metabolism Sequence Homology, Amino Acid Transformation, Genetic |
| title | Molecular analysis of pentachlorophenol degradation |
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