Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies
Chengcai Chu and colleagues show that genetic variation in NRT1.1B / OsNPF6.5 contributes to nitrate-use divergence between two main subspecies of Asian cultivated rice. Their findings may help to improve nitrogen-use efficiency in plant production. Asian cultivated rice ( Oryza sativa L.) consists...
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| Veröffentlicht in: | Nature genetics 2015-07, Vol.47 (7), p.834-838 |
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| creator | Hu, Bin Wang, Wei Ou, Shujun Tang, Jiuyou Li, Hua Che, Ronghui Zhang, Zhihua Chai, Xuyang Wang, Hongru Wang, Yiqin Liang, Chengzhen Liu, Linchuan Piao, Zhongze Deng, Qiyun Deng, Kun Xu, Chi Liang, Yan Zhang, Lianhe Li, Legong Chu, Chengcai |
| description | Chengcai Chu and colleagues show that genetic variation in
NRT1.1B
/
OsNPF6.5
contributes to nitrate-use divergence between two main subspecies of Asian cultivated rice. Their findings may help to improve nitrogen-use efficiency in plant production.
Asian cultivated rice (
Oryza sativa
L.) consists of two main subspecies,
indica
and
japonica
.
Indica
has higher nitrate-absorption activity than
japonica
, but the molecular mechanisms underlying that activity remain elusive. Here we show that variation in a nitrate-transporter gene,
NRT1.1B
(
OsNPF6.5
), may contribute to this divergence in nitrate use. Phylogenetic analysis revealed that
NRT1.1B
diverges between
indica
and
japonica
.
NRT1.1B
-
indica
variation was associated with enhanced nitrate uptake and root-to-shoot transport and upregulated expression of nitrate-responsive genes. The selection signature of
NRT1.1B
-
indica
suggests that nitrate-use divergence occurred during rice domestication. Notably, field tests with near-isogenic and transgenic lines confirmed that the
japonica
variety carrying the
NRT1.1B
-
indica
allele had significantly improved grain yield and nitrogen-use efficiency (NUE) compared to the variety without that allele. Our results show that variation in
NRT1.1B
largely explains nitrate-use divergence between
indica
and
japonica
and that
NRT1.1B
-
indica
can potentially improve the NUE of
japonica
. |
| doi_str_mv | 10.1038/ng.3337 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1691601921</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A421323414</galeid><sourcerecordid>A421323414</sourcerecordid><originalsourceid>FETCH-LOGICAL-c613t-eba79724b6c1ac35807eb9a4d5b626bf38499529292f9ac11e83eca4b1a6f3333</originalsourceid><addsrcrecordid>eNqNkktr3DAUhUVpyZv-g2LoounCE11Llq1lEtomEBqYPLZC0lwbhRl5Ksl9_PvKJGkyIYtyF3p996BzuIS8BzoDytoj388YY80bsgM1FyU00L7Neyqg5JSJbbIb4x2lwDltt8h2JWjNuGx2yPxWB6eTG3zhfPF9fg0zOCns4FNwZkwYizQU3qWgE5ZjxGLhfmLo0VssDKZfiL4ILh_iaOIarcO4T951ehnx4GHdIzdfv1yfnpUXl9_OT48vSiuApRKNbmRTcSMsaMvqljZopOaL2ohKmI61XMq6krk6qS0Atgyt5ga06LJZtkcO73XXYfgxYkxq5aLF5VJ7HMaoQEgQFGQFGf34Ar0bxuDz7yaKC5njkE9Ur5eonO-GbNtOouqYZ5WKceCZmr1C5VrgyuXgsHP5fqPh80bDFC7-Tr0eY1TnV_P_Zy9vN9lP96wNQ4wBO7UObqXDHwVUTVOhfK-mqcjkhwf7o1nh4h_3OAZPUcb85HsMz_J5ofUXnbW6Ng</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1694690539</pqid></control><display><type>article</type><title>Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies</title><source>MEDLINE</source><source>Nature</source><source>Springer Nature - Complete Springer Journals</source><creator>Hu, Bin ; Wang, Wei ; Ou, Shujun ; Tang, Jiuyou ; Li, Hua ; Che, Ronghui ; Zhang, Zhihua ; Chai, Xuyang ; Wang, Hongru ; Wang, Yiqin ; Liang, Chengzhen ; Liu, Linchuan ; Piao, Zhongze ; Deng, Qiyun ; Deng, Kun ; Xu, Chi ; Liang, Yan ; Zhang, Lianhe ; Li, Legong ; Chu, Chengcai</creator><creatorcontrib>Hu, Bin ; Wang, Wei ; Ou, Shujun ; Tang, Jiuyou ; Li, Hua ; Che, Ronghui ; Zhang, Zhihua ; Chai, Xuyang ; Wang, Hongru ; Wang, Yiqin ; Liang, Chengzhen ; Liu, Linchuan ; Piao, Zhongze ; Deng, Qiyun ; Deng, Kun ; Xu, Chi ; Liang, Yan ; Zhang, Lianhe ; Li, Legong ; Chu, Chengcai</creatorcontrib><description>Chengcai Chu and colleagues show that genetic variation in
NRT1.1B
/
OsNPF6.5
contributes to nitrate-use divergence between two main subspecies of Asian cultivated rice. Their findings may help to improve nitrogen-use efficiency in plant production.
Asian cultivated rice (
Oryza sativa
L.) consists of two main subspecies,
indica
and
japonica
.
Indica
has higher nitrate-absorption activity than
japonica
, but the molecular mechanisms underlying that activity remain elusive. Here we show that variation in a nitrate-transporter gene,
NRT1.1B
(
OsNPF6.5
), may contribute to this divergence in nitrate use. Phylogenetic analysis revealed that
NRT1.1B
diverges between
indica
and
japonica
.
NRT1.1B
-
indica
variation was associated with enhanced nitrate uptake and root-to-shoot transport and upregulated expression of nitrate-responsive genes. The selection signature of
NRT1.1B
-
indica
suggests that nitrate-use divergence occurred during rice domestication. Notably, field tests with near-isogenic and transgenic lines confirmed that the
japonica
variety carrying the
NRT1.1B
-
indica
allele had significantly improved grain yield and nitrogen-use efficiency (NUE) compared to the variety without that allele. Our results show that variation in
NRT1.1B
largely explains nitrate-use divergence between
indica
and
japonica
and that
NRT1.1B
-
indica
can potentially improve the NUE of
japonica
.</description><identifier>ISSN: 1061-4036</identifier><identifier>EISSN: 1546-1718</identifier><identifier>DOI: 10.1038/ng.3337</identifier><identifier>PMID: 26053497</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>45 ; 45/23 ; 631/449/2491 ; Agriculture ; Alleles ; Animal Genetics and Genomics ; Animals ; Anion Transport Proteins - genetics ; Anion Transport Proteins - metabolism ; Base Sequence ; Biological transport ; Biomedicine ; Cancer Research ; Cells, Cultured ; Cultivation ; Domestication ; Experiments ; Field tests ; Fixation ; Gene expression ; Gene Function ; Genetic aspects ; Genetic Speciation ; Genetic Variation ; Grain cultivation ; Human Genetics ; Identification and classification ; Labeling ; letter ; Nitrates ; Nitrates - metabolism ; Nitrogen ; Oryza - genetics ; Oryza - metabolism ; Phylogeny ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Polymorphism, Single Nucleotide ; Proteins ; Rice ; Species Specificity ; Transgenic plants ; Xenopus laevis</subject><ispartof>Nature genetics, 2015-07, Vol.47 (7), p.834-838</ispartof><rights>Springer Nature America, Inc. 2015</rights><rights>COPYRIGHT 2015 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jul 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c613t-eba79724b6c1ac35807eb9a4d5b626bf38499529292f9ac11e83eca4b1a6f3333</citedby><cites>FETCH-LOGICAL-c613t-eba79724b6c1ac35807eb9a4d5b626bf38499529292f9ac11e83eca4b1a6f3333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/ng.3337$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/ng.3337$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26053497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Bin</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Ou, Shujun</creatorcontrib><creatorcontrib>Tang, Jiuyou</creatorcontrib><creatorcontrib>Li, Hua</creatorcontrib><creatorcontrib>Che, Ronghui</creatorcontrib><creatorcontrib>Zhang, Zhihua</creatorcontrib><creatorcontrib>Chai, Xuyang</creatorcontrib><creatorcontrib>Wang, Hongru</creatorcontrib><creatorcontrib>Wang, Yiqin</creatorcontrib><creatorcontrib>Liang, Chengzhen</creatorcontrib><creatorcontrib>Liu, Linchuan</creatorcontrib><creatorcontrib>Piao, Zhongze</creatorcontrib><creatorcontrib>Deng, Qiyun</creatorcontrib><creatorcontrib>Deng, Kun</creatorcontrib><creatorcontrib>Xu, Chi</creatorcontrib><creatorcontrib>Liang, Yan</creatorcontrib><creatorcontrib>Zhang, Lianhe</creatorcontrib><creatorcontrib>Li, Legong</creatorcontrib><creatorcontrib>Chu, Chengcai</creatorcontrib><title>Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies</title><title>Nature genetics</title><addtitle>Nat Genet</addtitle><addtitle>Nat Genet</addtitle><description>Chengcai Chu and colleagues show that genetic variation in
NRT1.1B
/
OsNPF6.5
contributes to nitrate-use divergence between two main subspecies of Asian cultivated rice. Their findings may help to improve nitrogen-use efficiency in plant production.
Asian cultivated rice (
Oryza sativa
L.) consists of two main subspecies,
indica
and
japonica
.
Indica
has higher nitrate-absorption activity than
japonica
, but the molecular mechanisms underlying that activity remain elusive. Here we show that variation in a nitrate-transporter gene,
NRT1.1B
(
OsNPF6.5
), may contribute to this divergence in nitrate use. Phylogenetic analysis revealed that
NRT1.1B
diverges between
indica
and
japonica
.
NRT1.1B
-
indica
variation was associated with enhanced nitrate uptake and root-to-shoot transport and upregulated expression of nitrate-responsive genes. The selection signature of
NRT1.1B
-
indica
suggests that nitrate-use divergence occurred during rice domestication. Notably, field tests with near-isogenic and transgenic lines confirmed that the
japonica
variety carrying the
NRT1.1B
-
indica
allele had significantly improved grain yield and nitrogen-use efficiency (NUE) compared to the variety without that allele. Our results show that variation in
NRT1.1B
largely explains nitrate-use divergence between
indica
and
japonica
and that
NRT1.1B
-
indica
can potentially improve the NUE of
japonica
.</description><subject>45</subject><subject>45/23</subject><subject>631/449/2491</subject><subject>Agriculture</subject><subject>Alleles</subject><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Anion Transport Proteins - genetics</subject><subject>Anion Transport Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Biological transport</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Cells, Cultured</subject><subject>Cultivation</subject><subject>Domestication</subject><subject>Experiments</subject><subject>Field tests</subject><subject>Fixation</subject><subject>Gene expression</subject><subject>Gene Function</subject><subject>Genetic aspects</subject><subject>Genetic Speciation</subject><subject>Genetic Variation</subject><subject>Grain cultivation</subject><subject>Human Genetics</subject><subject>Identification and classification</subject><subject>Labeling</subject><subject>letter</subject><subject>Nitrates</subject><subject>Nitrates - metabolism</subject><subject>Nitrogen</subject><subject>Oryza - genetics</subject><subject>Oryza - metabolism</subject><subject>Phylogeny</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Proteins</subject><subject>Rice</subject><subject>Species Specificity</subject><subject>Transgenic plants</subject><subject>Xenopus laevis</subject><issn>1061-4036</issn><issn>1546-1718</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkktr3DAUhUVpyZv-g2LoounCE11Llq1lEtomEBqYPLZC0lwbhRl5Ksl9_PvKJGkyIYtyF3p996BzuIS8BzoDytoj388YY80bsgM1FyU00L7Neyqg5JSJbbIb4x2lwDltt8h2JWjNuGx2yPxWB6eTG3zhfPF9fg0zOCns4FNwZkwYizQU3qWgE5ZjxGLhfmLo0VssDKZfiL4ILh_iaOIarcO4T951ehnx4GHdIzdfv1yfnpUXl9_OT48vSiuApRKNbmRTcSMsaMvqljZopOaL2ohKmI61XMq6krk6qS0Atgyt5ga06LJZtkcO73XXYfgxYkxq5aLF5VJ7HMaoQEgQFGQFGf34Ar0bxuDz7yaKC5njkE9Ur5eonO-GbNtOouqYZ5WKceCZmr1C5VrgyuXgsHP5fqPh80bDFC7-Tr0eY1TnV_P_Zy9vN9lP96wNQ4wBO7UObqXDHwVUTVOhfK-mqcjkhwf7o1nh4h_3OAZPUcb85HsMz_J5ofUXnbW6Ng</recordid><startdate>20150701</startdate><enddate>20150701</enddate><creator>Hu, Bin</creator><creator>Wang, Wei</creator><creator>Ou, Shujun</creator><creator>Tang, Jiuyou</creator><creator>Li, Hua</creator><creator>Che, Ronghui</creator><creator>Zhang, Zhihua</creator><creator>Chai, Xuyang</creator><creator>Wang, Hongru</creator><creator>Wang, Yiqin</creator><creator>Liang, Chengzhen</creator><creator>Liu, Linchuan</creator><creator>Piao, Zhongze</creator><creator>Deng, Qiyun</creator><creator>Deng, Kun</creator><creator>Xu, Chi</creator><creator>Liang, Yan</creator><creator>Zhang, Lianhe</creator><creator>Li, Legong</creator><creator>Chu, Chengcai</creator><general>Nature Publishing Group US</general><general>Nature Publishing 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in NRT1.1B contributes to nitrate-use divergence between rice subspecies</title><author>Hu, Bin ; Wang, Wei ; Ou, Shujun ; Tang, Jiuyou ; Li, Hua ; Che, Ronghui ; Zhang, Zhihua ; Chai, Xuyang ; Wang, Hongru ; Wang, Yiqin ; Liang, Chengzhen ; Liu, Linchuan ; Piao, Zhongze ; Deng, Qiyun ; Deng, Kun ; Xu, Chi ; Liang, Yan ; Zhang, Lianhe ; Li, Legong ; Chu, Chengcai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c613t-eba79724b6c1ac35807eb9a4d5b626bf38499529292f9ac11e83eca4b1a6f3333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>45</topic><topic>45/23</topic><topic>631/449/2491</topic><topic>Agriculture</topic><topic>Alleles</topic><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Anion Transport Proteins - genetics</topic><topic>Anion Transport Proteins - metabolism</topic><topic>Base Sequence</topic><topic>Biological transport</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Cells, Cultured</topic><topic>Cultivation</topic><topic>Domestication</topic><topic>Experiments</topic><topic>Field tests</topic><topic>Fixation</topic><topic>Gene expression</topic><topic>Gene Function</topic><topic>Genetic aspects</topic><topic>Genetic Speciation</topic><topic>Genetic Variation</topic><topic>Grain cultivation</topic><topic>Human Genetics</topic><topic>Identification and classification</topic><topic>Labeling</topic><topic>letter</topic><topic>Nitrates</topic><topic>Nitrates - metabolism</topic><topic>Nitrogen</topic><topic>Oryza - genetics</topic><topic>Oryza - metabolism</topic><topic>Phylogeny</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Proteins</topic><topic>Rice</topic><topic>Species Specificity</topic><topic>Transgenic plants</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Bin</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Ou, Shujun</creatorcontrib><creatorcontrib>Tang, Jiuyou</creatorcontrib><creatorcontrib>Li, Hua</creatorcontrib><creatorcontrib>Che, Ronghui</creatorcontrib><creatorcontrib>Zhang, Zhihua</creatorcontrib><creatorcontrib>Chai, Xuyang</creatorcontrib><creatorcontrib>Wang, Hongru</creatorcontrib><creatorcontrib>Wang, Yiqin</creatorcontrib><creatorcontrib>Liang, Chengzhen</creatorcontrib><creatorcontrib>Liu, Linchuan</creatorcontrib><creatorcontrib>Piao, Zhongze</creatorcontrib><creatorcontrib>Deng, Qiyun</creatorcontrib><creatorcontrib>Deng, Kun</creatorcontrib><creatorcontrib>Xu, Chi</creatorcontrib><creatorcontrib>Liang, Yan</creatorcontrib><creatorcontrib>Zhang, Lianhe</creatorcontrib><creatorcontrib>Li, Legong</creatorcontrib><creatorcontrib>Chu, 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Chengcai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies</atitle><jtitle>Nature genetics</jtitle><stitle>Nat Genet</stitle><addtitle>Nat Genet</addtitle><date>2015-07-01</date><risdate>2015</risdate><volume>47</volume><issue>7</issue><spage>834</spage><epage>838</epage><pages>834-838</pages><issn>1061-4036</issn><eissn>1546-1718</eissn><abstract>Chengcai Chu and colleagues show that genetic variation in
NRT1.1B
/
OsNPF6.5
contributes to nitrate-use divergence between two main subspecies of Asian cultivated rice. Their findings may help to improve nitrogen-use efficiency in plant production.
Asian cultivated rice (
Oryza sativa
L.) consists of two main subspecies,
indica
and
japonica
.
Indica
has higher nitrate-absorption activity than
japonica
, but the molecular mechanisms underlying that activity remain elusive. Here we show that variation in a nitrate-transporter gene,
NRT1.1B
(
OsNPF6.5
), may contribute to this divergence in nitrate use. Phylogenetic analysis revealed that
NRT1.1B
diverges between
indica
and
japonica
.
NRT1.1B
-
indica
variation was associated with enhanced nitrate uptake and root-to-shoot transport and upregulated expression of nitrate-responsive genes. The selection signature of
NRT1.1B
-
indica
suggests that nitrate-use divergence occurred during rice domestication. Notably, field tests with near-isogenic and transgenic lines confirmed that the
japonica
variety carrying the
NRT1.1B
-
indica
allele had significantly improved grain yield and nitrogen-use efficiency (NUE) compared to the variety without that allele. Our results show that variation in
NRT1.1B
largely explains nitrate-use divergence between
indica
and
japonica
and that
NRT1.1B
-
indica
can potentially improve the NUE of
japonica
.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>26053497</pmid><doi>10.1038/ng.3337</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1061-4036 |
| ispartof | Nature genetics, 2015-07, Vol.47 (7), p.834-838 |
| issn | 1061-4036 1546-1718 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1691601921 |
| source | MEDLINE; Nature; Springer Nature - Complete Springer Journals |
| subjects | 45 45/23 631/449/2491 Agriculture Alleles Animal Genetics and Genomics Animals Anion Transport Proteins - genetics Anion Transport Proteins - metabolism Base Sequence Biological transport Biomedicine Cancer Research Cells, Cultured Cultivation Domestication Experiments Field tests Fixation Gene expression Gene Function Genetic aspects Genetic Speciation Genetic Variation Grain cultivation Human Genetics Identification and classification Labeling letter Nitrates Nitrates - metabolism Nitrogen Oryza - genetics Oryza - metabolism Phylogeny Plant Proteins - genetics Plant Proteins - metabolism Polymorphism, Single Nucleotide Proteins Rice Species Specificity Transgenic plants Xenopus laevis |
| title | Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T11%3A50%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Variation%20in%20NRT1.1B%20contributes%20to%20nitrate-use%20divergence%20between%20rice%20subspecies&rft.jtitle=Nature%20genetics&rft.au=Hu,%20Bin&rft.date=2015-07-01&rft.volume=47&rft.issue=7&rft.spage=834&rft.epage=838&rft.pages=834-838&rft.issn=1061-4036&rft.eissn=1546-1718&rft_id=info:doi/10.1038/ng.3337&rft_dat=%3Cgale_proqu%3EA421323414%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1694690539&rft_id=info:pmid/26053497&rft_galeid=A421323414&rfr_iscdi=true |