The impact of CRISPR–Cas9 on target identification and validation

•Cas9 has utility across gene editing especially for rapid knock-out (KO) generation.•The D10A nickase mutant may boost rates of homologous recombination.•The infrastructure developed for shRNA can be redeployed in sgRNA drop out screens.•sgRNA KO screens may overcome many of the issues with large-s...

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Veröffentlicht in:	Drug discovery today 2015-04, Vol.20 (4), p.450-457
1. Verfasser:	Moore, Jonathan D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Cas Systems Drug Discovery - methods Endonucleases - genetics Endonucleases - metabolism Gene Expression Regulation Gene Targeting - methods Genetic Predisposition to Disease Humans Phenotype
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