Otic placode cell specification and proliferation are regulated by Notch signaling in avian development
Background: The entire inner ear including the cochlear‐vestibular ganglion arises from a simple epithelium, the otic placode. Precursors for the placode originate from a pool of progenitors located in ectoderm next to the future hindbrain, the pre‐otic field, where they are intermingled with future...
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| Veröffentlicht in: | Developmental dynamics 2015-07, Vol.244 (7), p.839-851 |
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| creator | Shida, Hiroko Mende, Michael Takano‐Yamamoto, Teruko Osumi, Noriko Streit, Andrea Wakamatsu, Yoshio |
| description | Background: The entire inner ear including the cochlear‐vestibular ganglion arises from a simple epithelium, the otic placode. Precursors for the placode originate from a pool of progenitors located in ectoderm next to the future hindbrain, the pre‐otic field, where they are intermingled with future epibranchial and epidermal cells. While the importance of secreted proteins, such as FGFs and Wnts, in imparting otic identity has been well studied, how precursors for these different fates segregate locally is less well understood. Results: (1) The Notch ligand Delta1 and the Notch target Hes5‐2 are expressed in a part of pre‐otic field before otic commitment, indicative of active Notch signaling, and this is confirmed using a Notch reporter. (2) Loss and gain‐of‐function approaches reveal that Notch signaling regulates both proliferation and specification of pre‐otic progenitors. Conclusions: Our results identify a novel function of Notch signaling in cell fate determination in the pre‐otic field of avian embryos. Developmental Dynamics 244:839–851, 2015. © 2015 Wiley Periodicals, Inc.
Key Findings
The Notch ligand Delta1 and the Notch target Hes5‐2 are expressed in a part of pre‐otic field prior to otic commitment, indicative of active Notch signaling, and this is confirmed using a Notch reporter.
Loss and gain‐of‐function approaches reveal that Notch signaling regulates both proliferation and specification of pre‐otic progenitors. Our results identify a novel function of Notch signaling in cell fate determination in the pre‐otic field of avian embryos. |
| doi_str_mv | 10.1002/dvdy.24291 |
| format | Article |
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Key Findings
The Notch ligand Delta1 and the Notch target Hes5‐2 are expressed in a part of pre‐otic field prior to otic commitment, indicative of active Notch signaling, and this is confirmed using a Notch reporter.
Loss and gain‐of‐function approaches reveal that Notch signaling regulates both proliferation and specification of pre‐otic progenitors. Our results identify a novel function of Notch signaling in cell fate determination in the pre‐otic field of avian embryos.</description><identifier>ISSN: 1058-8388</identifier><identifier>EISSN: 1097-0177</identifier><identifier>DOI: 10.1002/dvdy.24291</identifier><identifier>PMID: 25970828</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animals ; Avian Proteins - metabolism ; Cell Proliferation - physiology ; Chick Embryo ; Chickens ; Coturnix - embryology ; Delta ; Ectoderm - cytology ; Ectoderm - embryology ; Hes ; Intracellular Signaling Peptides and Proteins - metabolism ; Membrane Proteins - metabolism ; Notch ; otic placode ; Pax2 ; Receptors, Notch - metabolism ; Signal Transduction - physiology ; Sox9 ; Spiral Ganglion - cytology ; Spiral Ganglion - embryology ; Stem Cells - cytology ; Stem Cells - metabolism</subject><ispartof>Developmental dynamics, 2015-07, Vol.244 (7), p.839-851</ispartof><rights>2015 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5291-259cef7ebb0c8d03801a18e2b1807614debc6fed6d6e4bab7e478dc782f1900c3</citedby><cites>FETCH-LOGICAL-c5291-259cef7ebb0c8d03801a18e2b1807614debc6fed6d6e4bab7e478dc782f1900c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fdvdy.24291$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fdvdy.24291$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,1434,27929,27930,45579,45580,46414,46838</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25970828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shida, Hiroko</creatorcontrib><creatorcontrib>Mende, Michael</creatorcontrib><creatorcontrib>Takano‐Yamamoto, Teruko</creatorcontrib><creatorcontrib>Osumi, Noriko</creatorcontrib><creatorcontrib>Streit, Andrea</creatorcontrib><creatorcontrib>Wakamatsu, Yoshio</creatorcontrib><title>Otic placode cell specification and proliferation are regulated by Notch signaling in avian development</title><title>Developmental dynamics</title><addtitle>Dev Dyn</addtitle><description>Background: The entire inner ear including the cochlear‐vestibular ganglion arises from a simple epithelium, the otic placode. Precursors for the placode originate from a pool of progenitors located in ectoderm next to the future hindbrain, the pre‐otic field, where they are intermingled with future epibranchial and epidermal cells. While the importance of secreted proteins, such as FGFs and Wnts, in imparting otic identity has been well studied, how precursors for these different fates segregate locally is less well understood. Results: (1) The Notch ligand Delta1 and the Notch target Hes5‐2 are expressed in a part of pre‐otic field before otic commitment, indicative of active Notch signaling, and this is confirmed using a Notch reporter. (2) Loss and gain‐of‐function approaches reveal that Notch signaling regulates both proliferation and specification of pre‐otic progenitors. Conclusions: Our results identify a novel function of Notch signaling in cell fate determination in the pre‐otic field of avian embryos. Developmental Dynamics 244:839–851, 2015. © 2015 Wiley Periodicals, Inc.
Key Findings
The Notch ligand Delta1 and the Notch target Hes5‐2 are expressed in a part of pre‐otic field prior to otic commitment, indicative of active Notch signaling, and this is confirmed using a Notch reporter.
Loss and gain‐of‐function approaches reveal that Notch signaling regulates both proliferation and specification of pre‐otic progenitors. Our results identify a novel function of Notch signaling in cell fate determination in the pre‐otic field of avian embryos.</description><subject>Animals</subject><subject>Avian Proteins - metabolism</subject><subject>Cell Proliferation - physiology</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>Coturnix - embryology</subject><subject>Delta</subject><subject>Ectoderm - cytology</subject><subject>Ectoderm - embryology</subject><subject>Hes</subject><subject>Intracellular Signaling Peptides and Proteins - metabolism</subject><subject>Membrane Proteins - metabolism</subject><subject>Notch</subject><subject>otic placode</subject><subject>Pax2</subject><subject>Receptors, Notch - metabolism</subject><subject>Signal Transduction - physiology</subject><subject>Sox9</subject><subject>Spiral Ganglion - cytology</subject><subject>Spiral Ganglion - embryology</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><issn>1058-8388</issn><issn>1097-0177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90E9LwzAYBvAgipvTix9AAl5E6EzaLkmPsvkPxF1U8FTS5O3MyNqatJN9ezM7PXjwlJfw4-F9H4ROKRlTQuIrvdabcZzGGd1DQ0oyHhHK-f52nohIJEIM0JH3S0KIYCk9RIN4knEiYjFEi3lrFG6sVLUGrMBa7BtQpjRKtqausKw0blxtTQlu9-MAO1h0VragcbHBT3Wr3rE3i0paUy2wCWZtZIU1rMHWzQqq9hgdlNJ6ONm9I_Rye_M8vY8e53cP0-vHSE3C_lFYTEHJoSiIEpokglBJBcQFFYQzmmooFCtBM80gLWTBIeVCKy7ikmaEqGSELvrcsPNHB77NV8Zvz5IV1J3PKcvoJBMsZoGe_6HLunPhhl5RliYsCeqyV8rV3jso88aZlXSbnJJ8W3--rT__rj_gs11kV6xA_9KfvgOgPfg0Fjb_ROWz19lbH_oFFzSREg</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Shida, Hiroko</creator><creator>Mende, Michael</creator><creator>Takano‐Yamamoto, Teruko</creator><creator>Osumi, Noriko</creator><creator>Streit, Andrea</creator><creator>Wakamatsu, Yoshio</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>JQ2</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201507</creationdate><title>Otic placode cell specification and proliferation are regulated by Notch signaling in avian development</title><author>Shida, Hiroko ; Mende, Michael ; Takano‐Yamamoto, Teruko ; Osumi, Noriko ; Streit, Andrea ; Wakamatsu, Yoshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5291-259cef7ebb0c8d03801a18e2b1807614debc6fed6d6e4bab7e478dc782f1900c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Avian Proteins - metabolism</topic><topic>Cell Proliferation - physiology</topic><topic>Chick Embryo</topic><topic>Chickens</topic><topic>Coturnix - embryology</topic><topic>Delta</topic><topic>Ectoderm - cytology</topic><topic>Ectoderm - embryology</topic><topic>Hes</topic><topic>Intracellular Signaling Peptides and Proteins - metabolism</topic><topic>Membrane Proteins - metabolism</topic><topic>Notch</topic><topic>otic placode</topic><topic>Pax2</topic><topic>Receptors, Notch - metabolism</topic><topic>Signal Transduction - physiology</topic><topic>Sox9</topic><topic>Spiral Ganglion - cytology</topic><topic>Spiral Ganglion - embryology</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shida, Hiroko</creatorcontrib><creatorcontrib>Mende, Michael</creatorcontrib><creatorcontrib>Takano‐Yamamoto, Teruko</creatorcontrib><creatorcontrib>Osumi, Noriko</creatorcontrib><creatorcontrib>Streit, Andrea</creatorcontrib><creatorcontrib>Wakamatsu, Yoshio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental dynamics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shida, Hiroko</au><au>Mende, Michael</au><au>Takano‐Yamamoto, Teruko</au><au>Osumi, Noriko</au><au>Streit, Andrea</au><au>Wakamatsu, Yoshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Otic placode cell specification and proliferation are regulated by Notch signaling in avian development</atitle><jtitle>Developmental dynamics</jtitle><addtitle>Dev Dyn</addtitle><date>2015-07</date><risdate>2015</risdate><volume>244</volume><issue>7</issue><spage>839</spage><epage>851</epage><pages>839-851</pages><issn>1058-8388</issn><eissn>1097-0177</eissn><abstract>Background: The entire inner ear including the cochlear‐vestibular ganglion arises from a simple epithelium, the otic placode. Precursors for the placode originate from a pool of progenitors located in ectoderm next to the future hindbrain, the pre‐otic field, where they are intermingled with future epibranchial and epidermal cells. While the importance of secreted proteins, such as FGFs and Wnts, in imparting otic identity has been well studied, how precursors for these different fates segregate locally is less well understood. Results: (1) The Notch ligand Delta1 and the Notch target Hes5‐2 are expressed in a part of pre‐otic field before otic commitment, indicative of active Notch signaling, and this is confirmed using a Notch reporter. (2) Loss and gain‐of‐function approaches reveal that Notch signaling regulates both proliferation and specification of pre‐otic progenitors. Conclusions: Our results identify a novel function of Notch signaling in cell fate determination in the pre‐otic field of avian embryos. Developmental Dynamics 244:839–851, 2015. © 2015 Wiley Periodicals, Inc.
Key Findings
The Notch ligand Delta1 and the Notch target Hes5‐2 are expressed in a part of pre‐otic field prior to otic commitment, indicative of active Notch signaling, and this is confirmed using a Notch reporter.
Loss and gain‐of‐function approaches reveal that Notch signaling regulates both proliferation and specification of pre‐otic progenitors. Our results identify a novel function of Notch signaling in cell fate determination in the pre‐otic field of avian embryos.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>25970828</pmid><doi>10.1002/dvdy.24291</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Avian Proteins - metabolism Cell Proliferation - physiology Chick Embryo Chickens Coturnix - embryology Delta Ectoderm - cytology Ectoderm - embryology Hes Intracellular Signaling Peptides and Proteins - metabolism Membrane Proteins - metabolism Notch otic placode Pax2 Receptors, Notch - metabolism Signal Transduction - physiology Sox9 Spiral Ganglion - cytology Spiral Ganglion - embryology Stem Cells - cytology Stem Cells - metabolism |
| title | Otic placode cell specification and proliferation are regulated by Notch signaling in avian development |
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