SAS-1 Is a C2 Domain Protein Critical for Centriole Integrity in C. elegans: e1004777
Centrioles are microtubule-based organelles important for the formation of cilia, flagella and centrosomes. Despite progress in understanding the underlying assembly mechanisms, how centriole integrity is ensured is incompletely understood, including in sperm cells, where such integrity is particula...
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| Veröffentlicht in: | PLoS genetics 2014-11, Vol.10 (11) |
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| container_title | PLoS genetics |
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| creator | Tobel, Lukas von Mikeladze-Dvali, Tamara Delattre, Marie Balestra, Fernando R Blanchoud, Simon Finger, Susanne Knott, Graham Müller-Reichert, Thomas Gönczy, Pierre |
| description | Centrioles are microtubule-based organelles important for the formation of cilia, flagella and centrosomes. Despite progress in understanding the underlying assembly mechanisms, how centriole integrity is ensured is incompletely understood, including in sperm cells, where such integrity is particularly critical. We identified C. elegans sas-1 in a genetic screen as a locus required for bipolar spindle assembly in the early embryo. Our analysis reveals that sperm-derived sas-1 mutant centrioles lose their integrity shortly after fertilization, and that a related defect occurs when maternal sas-1 function is lacking. We establish that sas-1 encodes a C2 domain containing protein that localizes to centrioles in C. elegans, and which can bind and stabilize microtubules when expressed in human cells. Moreover, we uncover that SAS-1 is related to C2CD3, a protein required for complete centriole formation in human cells and affected in a type of oral-facial-digital (OFD) syndrome. |
| doi_str_mv | 10.1371/journal.pgen.1004777 |
| format | Article |
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Moreover, we uncover that SAS-1 is related to C2CD3, a protein required for complete centriole formation in human cells and affected in a type of oral-facial-digital (OFD) syndrome.</description><identifier>ISSN: 1553-7390</identifier><identifier>EISSN: 1553-7404</identifier><identifier>DOI: 10.1371/journal.pgen.1004777</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Cell cycle ; Genes ; Motion pictures ; Nematodes</subject><ispartof>PLoS genetics, 2014-11, Vol.10 (11)</ispartof><rights>2014 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: . 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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central |
| subjects | Cell cycle Genes Motion pictures Nematodes |
| title | SAS-1 Is a C2 Domain Protein Critical for Centriole Integrity in C. elegans: e1004777 |
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