Expression and significance of MOC-31 and calretinin in pleural fluid of patients with lung cancer
Background The cytologic assessment of pleural effusions to distinguish carcinoma cells from reactive mesothelial cells is particularly challenging. The aim of this study was to investigate the diagnostic value of monoclonal antibody (MOC‐31) and calretinin in pleural fluid of patients with lung can...
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| Veröffentlicht in: | Diagnostic cytopathology 2015-07, Vol.43 (7), p.527-531 |
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| creator | Lv, Ming Leng, Ji-Hong Hao, Yan-Yong Sun, Yan Cha, Na Wu, Guang-Ping |
| description | Background
The cytologic assessment of pleural effusions to distinguish carcinoma cells from reactive mesothelial cells is particularly challenging. The aim of this study was to investigate the diagnostic value of monoclonal antibody (MOC‐31) and calretinin in pleural fluid of patients with lung cancer to significantly improve the diagnostic accuracy.
Methods
The expressions of MOC‐31 and calretinin were detected by means of S‐P immunocytochemical technique in pleural effusions of patients with lung cancer (n = 92) and in patients with benign lung disease (n = 70).
Results
The positive rate of MOC‐31 in pleural fluid was 90.2% (83/92) from patients with lung cancer and 2.9% (2/70) from patients with benign lung diseases, showing a significant difference (P < 0.01). Calretinin was expressed 87.1% (61/70) in benign lung diseases and 6.5% (6/92) in lung cancer, also showing a significant difference (P < 0.01). The optimal combination for assay was MOC‐31 + calretinin: Sensitivity and specificity were 100 and 98.6%, respectively.
Conclusion
MOC‐31 and calretinin are of important clinical value for diagnosing and differentially diagnosing the cancer cells in pleural fluid of patients with lung cancer. Diagn. Cytopathol. 2015;43:527–531. © 2014 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/dc.23218 |
| format | Article |
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The cytologic assessment of pleural effusions to distinguish carcinoma cells from reactive mesothelial cells is particularly challenging. The aim of this study was to investigate the diagnostic value of monoclonal antibody (MOC‐31) and calretinin in pleural fluid of patients with lung cancer to significantly improve the diagnostic accuracy.
Methods
The expressions of MOC‐31 and calretinin were detected by means of S‐P immunocytochemical technique in pleural effusions of patients with lung cancer (n = 92) and in patients with benign lung disease (n = 70).
Results
The positive rate of MOC‐31 in pleural fluid was 90.2% (83/92) from patients with lung cancer and 2.9% (2/70) from patients with benign lung diseases, showing a significant difference (P < 0.01). Calretinin was expressed 87.1% (61/70) in benign lung diseases and 6.5% (6/92) in lung cancer, also showing a significant difference (P < 0.01). The optimal combination for assay was MOC‐31 + calretinin: Sensitivity and specificity were 100 and 98.6%, respectively.
Conclusion
MOC‐31 and calretinin are of important clinical value for diagnosing and differentially diagnosing the cancer cells in pleural fluid of patients with lung cancer. Diagn. Cytopathol. 2015;43:527–531. © 2014 Wiley Periodicals, Inc.</description><identifier>ISSN: 8755-1039</identifier><identifier>EISSN: 1097-0339</identifier><identifier>DOI: 10.1002/dc.23218</identifier><identifier>PMID: 25346242</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Adenocarcinoma - diagnosis ; Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Adult ; Aged ; Aged, 80 and over ; Antibodies, Monoclonal - genetics ; Antibodies, Monoclonal - metabolism ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Calbindin 2 - genetics ; Calbindin 2 - metabolism ; calretinin ; Female ; Gene Expression ; Humans ; immunocytochemistry ; Immunohistochemistry ; Lung cancer ; lung neoplasms ; Lung Neoplasms - diagnosis ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Male ; Middle Aged ; MOC-31 ; Pleural Cavity - metabolism ; Pleural Cavity - pathology ; Pleural Effusion, Malignant - diagnosis ; Pleural Effusion, Malignant - genetics ; Pleural Effusion, Malignant - metabolism ; Pleural Effusion, Malignant - pathology ; pleural fluid ; Sensitivity and Specificity ; tumor markers</subject><ispartof>Diagnostic cytopathology, 2015-07, Vol.43 (7), p.527-531</ispartof><rights>2014 Wiley Periodicals, Inc.</rights><rights>2015 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3878-83ab7ad805db575dee91c1ebd3f6c64d0397f3da993b8b3ec6c29059fdc642e23</citedby><cites>FETCH-LOGICAL-c3878-83ab7ad805db575dee91c1ebd3f6c64d0397f3da993b8b3ec6c29059fdc642e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fdc.23218$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fdc.23218$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25346242$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lv, Ming</creatorcontrib><creatorcontrib>Leng, Ji-Hong</creatorcontrib><creatorcontrib>Hao, Yan-Yong</creatorcontrib><creatorcontrib>Sun, Yan</creatorcontrib><creatorcontrib>Cha, Na</creatorcontrib><creatorcontrib>Wu, Guang-Ping</creatorcontrib><title>Expression and significance of MOC-31 and calretinin in pleural fluid of patients with lung cancer</title><title>Diagnostic cytopathology</title><addtitle>Diagn. Cytopathol</addtitle><description>Background
The cytologic assessment of pleural effusions to distinguish carcinoma cells from reactive mesothelial cells is particularly challenging. The aim of this study was to investigate the diagnostic value of monoclonal antibody (MOC‐31) and calretinin in pleural fluid of patients with lung cancer to significantly improve the diagnostic accuracy.
Methods
The expressions of MOC‐31 and calretinin were detected by means of S‐P immunocytochemical technique in pleural effusions of patients with lung cancer (n = 92) and in patients with benign lung disease (n = 70).
Results
The positive rate of MOC‐31 in pleural fluid was 90.2% (83/92) from patients with lung cancer and 2.9% (2/70) from patients with benign lung diseases, showing a significant difference (P < 0.01). Calretinin was expressed 87.1% (61/70) in benign lung diseases and 6.5% (6/92) in lung cancer, also showing a significant difference (P < 0.01). The optimal combination for assay was MOC‐31 + calretinin: Sensitivity and specificity were 100 and 98.6%, respectively.
Conclusion
MOC‐31 and calretinin are of important clinical value for diagnosing and differentially diagnosing the cancer cells in pleural fluid of patients with lung cancer. Diagn. Cytopathol. 2015;43:527–531. © 2014 Wiley Periodicals, Inc.</description><subject>Adenocarcinoma - diagnosis</subject><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antibodies, Monoclonal - genetics</subject><subject>Antibodies, Monoclonal - metabolism</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Calbindin 2 - genetics</subject><subject>Calbindin 2 - metabolism</subject><subject>calretinin</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>immunocytochemistry</subject><subject>Immunohistochemistry</subject><subject>Lung cancer</subject><subject>lung neoplasms</subject><subject>Lung Neoplasms - diagnosis</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>MOC-31</subject><subject>Pleural Cavity - metabolism</subject><subject>Pleural Cavity - pathology</subject><subject>Pleural Effusion, Malignant - diagnosis</subject><subject>Pleural Effusion, Malignant - genetics</subject><subject>Pleural Effusion, Malignant - metabolism</subject><subject>Pleural Effusion, Malignant - pathology</subject><subject>pleural fluid</subject><subject>Sensitivity and Specificity</subject><subject>tumor markers</subject><issn>8755-1039</issn><issn>1097-0339</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10F1PFDEUgOGGYGBFE3-BacKNN4P92E7bS7IiGkESXYW7ptOewWK3M7YzAf69s8uCCYlJk16cJ2_ag9AbSo4oIey9d0eMM6p20IwSLSvCud5FMyWFqCjheh-9LOWGEKIZrffQPhN8XrM5m6Hm5K7PUEroErbJ4xKuU2iDs8kB7lp8frGoON2MnI0ZhpBCwtPpI4zZRtzGMfi17O0QIA0F34bhF45jusabSn6FXrQ2Fni9vQ_Qj48ny8Wn6uzi9PPi-KxyXElVKW4bab0iwjdCCg-gqaPQeN7Wrp776Ruy5d5qzRvVcHC1Y5oI3fppyoDxA_Tuodvn7s8IZTCrUBzEaBN0YzG01pRQSRWf6OEzetONOU2vWyvC5opq_i_ocldKhtb0OaxsvjeUmPXejXdms_eJvt0Gx2YF_gk-LnoC1QO4DRHu_xsyHxaPwa0PZYC7J2_zb1NLLoW5_HpqzpfLL9-uxHfzk_8FE8iZsA</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Lv, Ming</creator><creator>Leng, Ji-Hong</creator><creator>Hao, Yan-Yong</creator><creator>Sun, Yan</creator><creator>Cha, Na</creator><creator>Wu, Guang-Ping</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201507</creationdate><title>Expression and significance of MOC-31 and calretinin in pleural fluid of patients with lung cancer</title><author>Lv, Ming ; Leng, Ji-Hong ; Hao, Yan-Yong ; Sun, Yan ; Cha, Na ; Wu, Guang-Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3878-83ab7ad805db575dee91c1ebd3f6c64d0397f3da993b8b3ec6c29059fdc642e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adenocarcinoma - diagnosis</topic><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antibodies, Monoclonal - genetics</topic><topic>Antibodies, Monoclonal - metabolism</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Calbindin 2 - genetics</topic><topic>Calbindin 2 - metabolism</topic><topic>calretinin</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>immunocytochemistry</topic><topic>Immunohistochemistry</topic><topic>Lung cancer</topic><topic>lung neoplasms</topic><topic>Lung Neoplasms - diagnosis</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - pathology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>MOC-31</topic><topic>Pleural Cavity - metabolism</topic><topic>Pleural Cavity - pathology</topic><topic>Pleural Effusion, Malignant - diagnosis</topic><topic>Pleural Effusion, Malignant - genetics</topic><topic>Pleural Effusion, Malignant - metabolism</topic><topic>Pleural Effusion, Malignant - pathology</topic><topic>pleural fluid</topic><topic>Sensitivity and Specificity</topic><topic>tumor markers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lv, Ming</creatorcontrib><creatorcontrib>Leng, Ji-Hong</creatorcontrib><creatorcontrib>Hao, Yan-Yong</creatorcontrib><creatorcontrib>Sun, Yan</creatorcontrib><creatorcontrib>Cha, Na</creatorcontrib><creatorcontrib>Wu, Guang-Ping</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic cytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lv, Ming</au><au>Leng, Ji-Hong</au><au>Hao, Yan-Yong</au><au>Sun, Yan</au><au>Cha, Na</au><au>Wu, Guang-Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and significance of MOC-31 and calretinin in pleural fluid of patients with lung cancer</atitle><jtitle>Diagnostic cytopathology</jtitle><addtitle>Diagn. Cytopathol</addtitle><date>2015-07</date><risdate>2015</risdate><volume>43</volume><issue>7</issue><spage>527</spage><epage>531</epage><pages>527-531</pages><issn>8755-1039</issn><eissn>1097-0339</eissn><abstract>Background
The cytologic assessment of pleural effusions to distinguish carcinoma cells from reactive mesothelial cells is particularly challenging. The aim of this study was to investigate the diagnostic value of monoclonal antibody (MOC‐31) and calretinin in pleural fluid of patients with lung cancer to significantly improve the diagnostic accuracy.
Methods
The expressions of MOC‐31 and calretinin were detected by means of S‐P immunocytochemical technique in pleural effusions of patients with lung cancer (n = 92) and in patients with benign lung disease (n = 70).
Results
The positive rate of MOC‐31 in pleural fluid was 90.2% (83/92) from patients with lung cancer and 2.9% (2/70) from patients with benign lung diseases, showing a significant difference (P < 0.01). Calretinin was expressed 87.1% (61/70) in benign lung diseases and 6.5% (6/92) in lung cancer, also showing a significant difference (P < 0.01). The optimal combination for assay was MOC‐31 + calretinin: Sensitivity and specificity were 100 and 98.6%, respectively.
Conclusion
MOC‐31 and calretinin are of important clinical value for diagnosing and differentially diagnosing the cancer cells in pleural fluid of patients with lung cancer. Diagn. Cytopathol. 2015;43:527–531. © 2014 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25346242</pmid><doi>10.1002/dc.23218</doi><tpages>5</tpages></addata></record> |
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| subjects | Adenocarcinoma - diagnosis Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - pathology Adult Aged Aged, 80 and over Antibodies, Monoclonal - genetics Antibodies, Monoclonal - metabolism Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Calbindin 2 - genetics Calbindin 2 - metabolism calretinin Female Gene Expression Humans immunocytochemistry Immunohistochemistry Lung cancer lung neoplasms Lung Neoplasms - diagnosis Lung Neoplasms - genetics Lung Neoplasms - metabolism Lung Neoplasms - pathology Male Middle Aged MOC-31 Pleural Cavity - metabolism Pleural Cavity - pathology Pleural Effusion, Malignant - diagnosis Pleural Effusion, Malignant - genetics Pleural Effusion, Malignant - metabolism Pleural Effusion, Malignant - pathology pleural fluid Sensitivity and Specificity tumor markers |
| title | Expression and significance of MOC-31 and calretinin in pleural fluid of patients with lung cancer |
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