Insecticide resistance genes in Culex pipiens (Diptera: Culicidae) from Italy: esterase B locus at the DNA level
Restriction fragment length polymorphism (RFLP) analysis at the esterase B locus was performed in samples from four populations of Italian Culex pipiens L. to identify correctly, at the molecular level, the resistance gene. The absence of the amplified B2 haplotype was confirmed, indicating that it...
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| Veröffentlicht in: | Journal of medical entomology 1994-05, Vol.31 (3), p.496-499 |
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| container_title | Journal of medical entomology |
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| creator | Severini, C Marinucci, M Raymond, M |
| description | Restriction fragment length polymorphism (RFLP) analysis at the esterase B locus was performed in samples from four populations of Italian Culex pipiens L. to identify correctly, at the molecular level, the resistance gene. The absence of the amplified B2 haplotype was confirmed, indicating that it has not increased in frequency after its detection in continental Italy around 1985. The B4 haplotype was detected in all populations at the same frequencies as a previous starch gel survey. The possibility that B4 amplification has occurred only once and has migrated across western Mediterranean region is supported by the data. A1 also was detected indirectly by the presence of its closely linked B allele. The B5 haplotype apparently was absent. No amplification at the esterase B locus was detected, except B4, indicating a low rate of occurrence of new amplification at this locus. |
| doi_str_mv | 10.1093/jmedent/31.3.496 |
| format | Article |
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The absence of the amplified B2 haplotype was confirmed, indicating that it has not increased in frequency after its detection in continental Italy around 1985. The B4 haplotype was detected in all populations at the same frequencies as a previous starch gel survey. The possibility that B4 amplification has occurred only once and has migrated across western Mediterranean region is supported by the data. A1 also was detected indirectly by the presence of its closely linked B allele. The B5 haplotype apparently was absent. No amplification at the esterase B locus was detected, except B4, indicating a low rate of occurrence of new amplification at this locus.</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 1938-2928</identifier><identifier>DOI: 10.1093/jmedent/31.3.496</identifier><identifier>PMID: 7914542</identifier><identifier>CODEN: JMENA6</identifier><language>eng</language><publisher>Lanham, MD: Entomological Society of America</publisher><subject>Animals ; Biological and medical sciences ; Culex - enzymology ; Culex - genetics ; Culex pipiens ; Culex pipiens pipiens ; Culicidae ; Diptera ; DNA - genetics ; dna amplification ; enzyme activity ; esterases ; Fundamental and applied biological sciences. Psychology ; Gene Frequency ; genes ; Genes, Insect ; Insecticide Resistance ; Insecticides - pharmacology ; Invertebrates ; Italy ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; Organophosphorus Compounds ; Polymorphism, Restriction Fragment Length ; population ; restriction fragment length polymorphism ; Serine Endopeptidases - genetics ; Vectors. Intermediate hosts</subject><ispartof>Journal of medical entomology, 1994-05, Vol.31 (3), p.496-499</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-94a31c8842487a6750ad9326cfbb4003fdfbfbddbb75671f4222c24a0d0d1cf93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4227345$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7914542$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Severini, C</creatorcontrib><creatorcontrib>Marinucci, M</creatorcontrib><creatorcontrib>Raymond, M</creatorcontrib><title>Insecticide resistance genes in Culex pipiens (Diptera: Culicidae) from Italy: esterase B locus at the DNA level</title><title>Journal of medical entomology</title><addtitle>J Med Entomol</addtitle><description>Restriction fragment length polymorphism (RFLP) analysis at the esterase B locus was performed in samples from four populations of Italian Culex pipiens L. to identify correctly, at the molecular level, the resistance gene. The absence of the amplified B2 haplotype was confirmed, indicating that it has not increased in frequency after its detection in continental Italy around 1985. The B4 haplotype was detected in all populations at the same frequencies as a previous starch gel survey. The possibility that B4 amplification has occurred only once and has migrated across western Mediterranean region is supported by the data. A1 also was detected indirectly by the presence of its closely linked B allele. The B5 haplotype apparently was absent. No amplification at the esterase B locus was detected, except B4, indicating a low rate of occurrence of new amplification at this locus.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Culex - enzymology</subject><subject>Culex - genetics</subject><subject>Culex pipiens</subject><subject>Culex pipiens pipiens</subject><subject>Culicidae</subject><subject>Diptera</subject><subject>DNA - genetics</subject><subject>dna amplification</subject><subject>enzyme activity</subject><subject>esterases</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Frequency</subject><subject>genes</subject><subject>Genes, Insect</subject><subject>Insecticide Resistance</subject><subject>Insecticides - pharmacology</subject><subject>Invertebrates</subject><subject>Italy</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>Organophosphorus Compounds</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>population</subject><subject>restriction fragment length polymorphism</subject><subject>Serine Endopeptidases - genetics</subject><subject>Vectors. Intermediate hosts</subject><issn>0022-2585</issn><issn>1938-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kUtv1DAURi0EKkPLng3CC4Rgken1Iw93V6bQjlTBArq2HOe6uPIkwU5Q--9xNFFXlvyd7_rqmJB3DLYMlDh_OGCH_XQu2FZspapekA1Toim44s1LsgHgvOBlU74mb1J6AICGSXVCTmrFZCn5hoz7PqGdvPUd0ojJp8n0Fuk99pio7-luDvhIRz967BP9fOXHCaO5WO6XksEv1MXhQPeTCU8XFNMSJ6RfaRjsnKiZ6PQH6dWPSxrwH4Yz8sqZkPDtep6Su-_ffu9uituf1_vd5W1hRd1MhZJGMNs0ksumNlVdgumU4JV1bSsBhOtc69qua9u6rGrmJOfccmmgg45Zp8Qp-XScO8bh75zX0gefLIZgehzmpFmlGLCyzCAcQRuHlCI6PUZ_MPFJM9CLZL1K1oJpobPkXHm_zp7bnD0XVqs5_7jmJlkTXMxKfXrG8q61kMvLH46YM4M29zEjd784MAH5k6ACLv4DifuPkg</recordid><startdate>19940501</startdate><enddate>19940501</enddate><creator>Severini, C</creator><creator>Marinucci, M</creator><creator>Raymond, M</creator><general>Entomological Society of America</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19940501</creationdate><title>Insecticide resistance genes in Culex pipiens (Diptera: Culicidae) from Italy: esterase B locus at the DNA level</title><author>Severini, C ; Marinucci, M ; Raymond, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-94a31c8842487a6750ad9326cfbb4003fdfbfbddbb75671f4222c24a0d0d1cf93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Culex - enzymology</topic><topic>Culex - genetics</topic><topic>Culex pipiens</topic><topic>Culex pipiens pipiens</topic><topic>Culicidae</topic><topic>Diptera</topic><topic>DNA - genetics</topic><topic>dna amplification</topic><topic>enzyme activity</topic><topic>esterases</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Frequency</topic><topic>genes</topic><topic>Genes, Insect</topic><topic>Insecticide Resistance</topic><topic>Insecticides - pharmacology</topic><topic>Invertebrates</topic><topic>Italy</topic><topic>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</topic><topic>Organophosphorus Compounds</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>population</topic><topic>restriction fragment length polymorphism</topic><topic>Serine Endopeptidases - genetics</topic><topic>Vectors. Intermediate hosts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Severini, C</creatorcontrib><creatorcontrib>Marinucci, M</creatorcontrib><creatorcontrib>Raymond, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of medical entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Severini, C</au><au>Marinucci, M</au><au>Raymond, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insecticide resistance genes in Culex pipiens (Diptera: Culicidae) from Italy: esterase B locus at the DNA level</atitle><jtitle>Journal of medical entomology</jtitle><addtitle>J Med Entomol</addtitle><date>1994-05-01</date><risdate>1994</risdate><volume>31</volume><issue>3</issue><spage>496</spage><epage>499</epage><pages>496-499</pages><issn>0022-2585</issn><eissn>1938-2928</eissn><coden>JMENA6</coden><abstract>Restriction fragment length polymorphism (RFLP) analysis at the esterase B locus was performed in samples from four populations of Italian Culex pipiens L. to identify correctly, at the molecular level, the resistance gene. The absence of the amplified B2 haplotype was confirmed, indicating that it has not increased in frequency after its detection in continental Italy around 1985. The B4 haplotype was detected in all populations at the same frequencies as a previous starch gel survey. The possibility that B4 amplification has occurred only once and has migrated across western Mediterranean region is supported by the data. A1 also was detected indirectly by the presence of its closely linked B allele. The B5 haplotype apparently was absent. No amplification at the esterase B locus was detected, except B4, indicating a low rate of occurrence of new amplification at this locus.</abstract><cop>Lanham, MD</cop><pub>Entomological Society of America</pub><pmid>7914542</pmid><doi>10.1093/jmedent/31.3.496</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of medical entomology, 1994-05, Vol.31 (3), p.496-499 |
| issn | 0022-2585 1938-2928 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | Animals Biological and medical sciences Culex - enzymology Culex - genetics Culex pipiens Culex pipiens pipiens Culicidae Diptera DNA - genetics dna amplification enzyme activity esterases Fundamental and applied biological sciences. Psychology Gene Frequency genes Genes, Insect Insecticide Resistance Insecticides - pharmacology Invertebrates Italy Medically important nuisances and vectors, pests of stored products and materials: population survey and control Organophosphorus Compounds Polymorphism, Restriction Fragment Length population restriction fragment length polymorphism Serine Endopeptidases - genetics Vectors. Intermediate hosts |
| title | Insecticide resistance genes in Culex pipiens (Diptera: Culicidae) from Italy: esterase B locus at the DNA level |
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