Treatment of Agrobacterium or leaf disks with 5-azacytidine increases transgene expression in tobacco

We have studied the effect of the demethylating agent azacytidine (azaC) on expression of a beta-glucuronidase (GUS) gene transferred to tobacco leaf disks by Agrobacterium-mediated transformation. In a system where no selection was performed, where shoot formation was partially repressed, and where...

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Veröffentlicht in:	Plant molecular biology 1993-02, Vol.21 (3), p.429-435
Hauptverfasser:	Palmgren, G. (Copenhagen Univ. (Denmark). Inst. of Plant Physiology), Mattson, O, Okkels, F.T
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN AGROBACTERIUM Agrobacterium tumefaciens Agrobacterium tumefaciens - genetics Azacitidine - pharmacology Biological and medical sciences Biotechnology DNA EXPRESION GENICA EXPRESSION DES GENES FEUILLE Fundamental and applied biological sciences. Psychology GENE EXPRESSION Gene Expression Regulation - drug effects Genetic engineering Genetic technics GENETIC TRANSFORMATION Glucuronidase - genetics HOJAS LEAVES Methods. Procedures. Technologies METHYLATION Methylation - drug effects METILACION NICOTIANA Nicotiana - genetics Nicotiana tabacum Plants, Genetically Modified - drug effects Plants, Toxic TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transformation, Genetic - drug effects Transgenic animals and transgenic plants Transgenic plants
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