Effects of steroids on association of T-2 toxin with mammalian cells
The effects of steroids on the association of T-2 toxin with cultured cells were evaluated. Preincubating cells with certain steroids led to a time- and concentration-related increase in total T-2-cell association. At maximally effective concentrations, the increase in association was 300–500%. This...
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| Veröffentlicht in: | Toxicon (Oxford) 1994-04, Vol.32 (4), p.435-444 |
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| creator | Middlebrook, John L. Leatherman, Dennis L. |
| description | The effects of steroids on the association of T-2 toxin with cultured cells were evaluated. Preincubating cells with certain steroids led to a time- and concentration-related increase in total T-2-cell association. At maximally effective concentrations, the increase in association was 300–500%. This effect required a preincubation at 37°C for a minimum of 10 min and was completely reversible after 20–30 min. Steroid treatment increased the rate of toxin-cell association and decreased the rate of dissociation. The effect was elicited by progesterone, estradiol, testosterone and diethylstilbestrol, but not by several other steroids tested. Binding of T-2 to isolated ribosomes was not altered by the steroids. We speculated that steroids somehow alter the state of ribosomal aggregation or assembly such that more toxin can bind after entering the cell. |
| doi_str_mv | 10.1016/0041-0101(94)90295-X |
| format | Article |
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Preincubating cells with certain steroids led to a time- and concentration-related increase in total T-2-cell association. At maximally effective concentrations, the increase in association was 300–500%. This effect required a preincubation at 37°C for a minimum of 10 min and was completely reversible after 20–30 min. Steroid treatment increased the rate of toxin-cell association and decreased the rate of dissociation. The effect was elicited by progesterone, estradiol, testosterone and diethylstilbestrol, but not by several other steroids tested. Binding of T-2 to isolated ribosomes was not altered by the steroids. 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Preincubating cells with certain steroids led to a time- and concentration-related increase in total T-2-cell association. At maximally effective concentrations, the increase in association was 300–500%. This effect required a preincubation at 37°C for a minimum of 10 min and was completely reversible after 20–30 min. Steroid treatment increased the rate of toxin-cell association and decreased the rate of dissociation. The effect was elicited by progesterone, estradiol, testosterone and diethylstilbestrol, but not by several other steroids tested. Binding of T-2 to isolated ribosomes was not altered by the steroids. We speculated that steroids somehow alter the state of ribosomal aggregation or assembly such that more toxin can bind after entering the cell.</description><subject>Animals</subject><subject>binding</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>cell lines</subject><subject>Cell Membrane - drug effects</subject><subject>Chinese hamsters</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>diethylstilbestrol</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Interactions</subject><subject>estradiol</subject><subject>Female</subject><subject>Kinetics</subject><subject>Medical sciences</subject><subject>ovaries</subject><subject>Plant poisons toxicology</subject><subject>progesterone</subject><subject>Progesterone - pharmacology</subject><subject>Protein Biosynthesis</subject><subject>protein synthesis</subject><subject>ribosomes</subject><subject>Ribosomes - drug effects</subject><subject>Ribosomes - metabolism</subject><subject>steroids</subject><subject>Steroids - pharmacology</subject><subject>T-2 toxin</subject><subject>T-2 Toxin - metabolism</subject><subject>testosterone</subject><subject>Toxicology</subject><subject>uptake</subject><subject>Vero Cells</subject><issn>0041-0101</issn><issn>1879-3150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1rFTEUhkNR6u3HP1CchZS6GD35mEzORpB-2EKhC1voLmQyiUZmJjWZa-u_b8Z7uUtXeeE85-XwhJC3FD5RoPIzgKA1lHiK4iMCw6Z-2CMrqlqsOW3gFVntkDfkIOdfAMAVyn2yr6BhiGpFzi-8d3bOVfRVnl2KoS95qkzO0QYzh5LL6K5m1Ryfw1Q9hflnNZpxNEMwU2XdMOQj8tqbIbvj7XtI7i8v7s6u6pvbb9dnX29qKwSd645xJp0UgimFfYfMYgtoe28UF6prur6XTAnDG_QNl8iEazi0LUrTtlJQfkhONr2PKf5euzzrMeTlAjO5uM6aSoVKSFlAsQFtijkn5_VjCqNJfzUFvcjTixm9mNEo9D95-qGsvdv2r7vR9bulra0y_7Cdm2zN4JOZbMg7TICkCFiw9xvMm6jNj1SQ--8MKAcqkCFdir5sCFds_Qku6WyDm6zrQyq_ofsY_n_pC9qokbE</recordid><startdate>19940401</startdate><enddate>19940401</enddate><creator>Middlebrook, John L.</creator><creator>Leatherman, Dennis L.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>M7N</scope></search><sort><creationdate>19940401</creationdate><title>Effects of steroids on association of T-2 toxin with mammalian cells</title><author>Middlebrook, John L. ; Leatherman, Dennis L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-b2326e6442889db92c9709cdfa8348b5bdd6284a359f536924e5307796a776413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>binding</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>cell lines</topic><topic>Cell Membrane - drug effects</topic><topic>Chinese hamsters</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>diethylstilbestrol</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Interactions</topic><topic>estradiol</topic><topic>Female</topic><topic>Kinetics</topic><topic>Medical sciences</topic><topic>ovaries</topic><topic>Plant poisons toxicology</topic><topic>progesterone</topic><topic>Progesterone - pharmacology</topic><topic>Protein Biosynthesis</topic><topic>protein synthesis</topic><topic>ribosomes</topic><topic>Ribosomes - drug effects</topic><topic>Ribosomes - metabolism</topic><topic>steroids</topic><topic>Steroids - pharmacology</topic><topic>T-2 toxin</topic><topic>T-2 Toxin - metabolism</topic><topic>testosterone</topic><topic>Toxicology</topic><topic>uptake</topic><topic>Vero Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Middlebrook, John L.</creatorcontrib><creatorcontrib>Leatherman, Dennis L.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Toxicon (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Middlebrook, John L.</au><au>Leatherman, Dennis L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of steroids on association of T-2 toxin with mammalian cells</atitle><jtitle>Toxicon (Oxford)</jtitle><addtitle>Toxicon</addtitle><date>1994-04-01</date><risdate>1994</risdate><volume>32</volume><issue>4</issue><spage>435</spage><epage>444</epage><pages>435-444</pages><issn>0041-0101</issn><eissn>1879-3150</eissn><coden>TOXIA6</coden><abstract>The effects of steroids on the association of T-2 toxin with cultured cells were evaluated. Preincubating cells with certain steroids led to a time- and concentration-related increase in total T-2-cell association. At maximally effective concentrations, the increase in association was 300–500%. This effect required a preincubation at 37°C for a minimum of 10 min and was completely reversible after 20–30 min. Steroid treatment increased the rate of toxin-cell association and decreased the rate of dissociation. The effect was elicited by progesterone, estradiol, testosterone and diethylstilbestrol, but not by several other steroids tested. Binding of T-2 to isolated ribosomes was not altered by the steroids. We speculated that steroids somehow alter the state of ribosomal aggregation or assembly such that more toxin can bind after entering the cell.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8052998</pmid><doi>10.1016/0041-0101(94)90295-X</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Toxicon (Oxford), 1994-04, Vol.32 (4), p.435-444 |
| issn | 0041-0101 1879-3150 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals binding Biological and medical sciences Cell Line cell lines Cell Membrane - drug effects Chinese hamsters CHO Cells Cricetinae Cricetulus diethylstilbestrol Dose-Response Relationship, Drug Drug Interactions estradiol Female Kinetics Medical sciences ovaries Plant poisons toxicology progesterone Progesterone - pharmacology Protein Biosynthesis protein synthesis ribosomes Ribosomes - drug effects Ribosomes - metabolism steroids Steroids - pharmacology T-2 toxin T-2 Toxin - metabolism testosterone Toxicology uptake Vero Cells |
| title | Effects of steroids on association of T-2 toxin with mammalian cells |
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