Levels of five mycotoxins in grains harvested in Atlantic Canada as measured by high performance liquid chromatography
An analytical protocol using high performance liquid chromatography (HPLC) was used to analyze samples of spring wheat, winter wheat, 2-row barley, and 6-row barley over a period of three years for the presence of five mycotoxins. These included deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, an...
Gespeichert in:
| Veröffentlicht in: | Archives of environmental contamination and toxicology 1993-04, Vol.24 (3), p.399-409 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 409 |
|---|---|
| container_issue | 3 |
| container_start_page | 399 |
| container_title | Archives of environmental contamination and toxicology |
| container_volume | 24 |
| creator | Stratton, G.W. (Department of Biology, Nova Scotia, Canada.) Robinson, A.R Smith, H.C Kittilsen, L Barbour, M |
| description | An analytical protocol using high performance liquid chromatography (HPLC) was used to analyze samples of spring wheat, winter wheat, 2-row barley, and 6-row barley over a period of three years for the presence of five mycotoxins. These included deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, and diacetoxyscirpenol. The protocol employed a single extraction step using acetonitrile-water and two cleanup procedures. One utilized a solid-phase extraction column and the other a charcoal-alumina column. Detection limits ranged from 0.02 to 0.15 micrograms of mycotoxin g-1 grain. Little T-2 toxin, HT-2 toxin, or diacetoxyscirpenol was found in the samples. Deoxynivalenol was detected in 53 to 62% of the samples tested and zearalenone in 25-29% of the samples. Several enzyme-linked immunosorbent assays were used for comparison purposes with deoxynivalenol, zearalenone, and T-2 toxin. These kits provided reliable qualitative, but not quantitative, data. |
| doi_str_mv | 10.1007/BF01128740 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16893122</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16893122</sourcerecordid><originalsourceid>FETCH-LOGICAL-c307t-2643dc175b6bb9033ac1fb91527f00199cef28a73a22d4866cdccd1120e46d063</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxS0EKkvhwhEJyQfEASll_NE4PpYVpZVW4lB6jib-2Bgl8dZOVux_j1e7KkdOHvn99EbvDSHvGVwxAPX12y0wxhsl4QVZMSl4BQrES7IC0FBJIdlr8ibn3wCFauQFuWikAi2aFdlv3N4NmUZPfdg7Oh5MnOOfMGUaJrpNeJx6THuXZ2ePfzfzgNMcDF3jhBYpZjo6zEsqcnegfdj2dOeSj2nEyTg6hKclWGr6FEecY7Hc9Ye35JXHIbt35_eSPN5-_7W-qzY_f9yvbzaVEaDmitdSWMPUdVd3nQYh0DDfaXbNlS9htDbO8waVQM6tbOraWGNs6QKcrC3U4pJ8PvnuUnxaSoZ2DNm4oURwccktqxstGOf_ByXXSrKmgF9OoEkx5-R8u0thxHRoGbTHa7T_rlHgj2fXpRudfUbP9Rf901nHbHDwqTQW8jMmVdnKVME-nDCPscVtKsjjg645SCbEXwO4meA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14297418</pqid></control><display><type>article</type><title>Levels of five mycotoxins in grains harvested in Atlantic Canada as measured by high performance liquid chromatography</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Stratton, G.W. (Department of Biology, Nova Scotia, Canada.) ; Robinson, A.R ; Smith, H.C ; Kittilsen, L ; Barbour, M</creator><creatorcontrib>Stratton, G.W. (Department of Biology, Nova Scotia, Canada.) ; Robinson, A.R ; Smith, H.C ; Kittilsen, L ; Barbour, M</creatorcontrib><description>An analytical protocol using high performance liquid chromatography (HPLC) was used to analyze samples of spring wheat, winter wheat, 2-row barley, and 6-row barley over a period of three years for the presence of five mycotoxins. These included deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, and diacetoxyscirpenol. The protocol employed a single extraction step using acetonitrile-water and two cleanup procedures. One utilized a solid-phase extraction column and the other a charcoal-alumina column. Detection limits ranged from 0.02 to 0.15 micrograms of mycotoxin g-1 grain. Little T-2 toxin, HT-2 toxin, or diacetoxyscirpenol was found in the samples. Deoxynivalenol was detected in 53 to 62% of the samples tested and zearalenone in 25-29% of the samples. Several enzyme-linked immunosorbent assays were used for comparison purposes with deoxynivalenol, zearalenone, and T-2 toxin. These kits provided reliable qualitative, but not quantitative, data.</description><identifier>ISSN: 0090-4341</identifier><identifier>EISSN: 1432-0703</identifier><identifier>DOI: 10.1007/BF01128740</identifier><identifier>PMID: 8470938</identifier><identifier>CODEN: AECTCV</identifier><language>eng</language><publisher>Heidelberg: Springer-Verlag</publisher><subject>Agronomy. Soil science and plant productions ; ALIMENTOS ; ANALYTICAL METHODS ; BARLEY ; Biological and medical sciences ; BLE ; Canada ; CEBADA ; CHEMICAL COMPOSITION ; Chromatography, High Pressure Liquid ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; CONTAMINACION ; CONTAMINATION ; DETECTION ; Edible Grain - chemistry ; Food Contamination - analysis ; Food industries ; FOOD SAFETY ; Food toxicology ; FOODS ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; HPLC ; INNOCUITE DES PRODUITS ALIMENTAIRES ; INOCUIDAD ALIMENTARIA ; MICOTOXINAS ; MYCOTOXINE ; MYCOTOXINS ; Mycotoxins - analysis ; ORGE ; Pathology, epidemiology, host-fungus relationships. Damages, economic importance ; Phytopathology. Animal pests. Plant and forest protection ; PRODUIT ALIMENTAIRE ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; TRIGO ; WHEATS</subject><ispartof>Archives of environmental contamination and toxicology, 1993-04, Vol.24 (3), p.399-409</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c307t-2643dc175b6bb9033ac1fb91527f00199cef28a73a22d4866cdccd1120e46d063</citedby><cites>FETCH-LOGICAL-c307t-2643dc175b6bb9033ac1fb91527f00199cef28a73a22d4866cdccd1120e46d063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4742917$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8470938$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stratton, G.W. (Department of Biology, Nova Scotia, Canada.)</creatorcontrib><creatorcontrib>Robinson, A.R</creatorcontrib><creatorcontrib>Smith, H.C</creatorcontrib><creatorcontrib>Kittilsen, L</creatorcontrib><creatorcontrib>Barbour, M</creatorcontrib><title>Levels of five mycotoxins in grains harvested in Atlantic Canada as measured by high performance liquid chromatography</title><title>Archives of environmental contamination and toxicology</title><addtitle>Arch Environ Contam Toxicol</addtitle><description>An analytical protocol using high performance liquid chromatography (HPLC) was used to analyze samples of spring wheat, winter wheat, 2-row barley, and 6-row barley over a period of three years for the presence of five mycotoxins. These included deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, and diacetoxyscirpenol. The protocol employed a single extraction step using acetonitrile-water and two cleanup procedures. One utilized a solid-phase extraction column and the other a charcoal-alumina column. Detection limits ranged from 0.02 to 0.15 micrograms of mycotoxin g-1 grain. Little T-2 toxin, HT-2 toxin, or diacetoxyscirpenol was found in the samples. Deoxynivalenol was detected in 53 to 62% of the samples tested and zearalenone in 25-29% of the samples. Several enzyme-linked immunosorbent assays were used for comparison purposes with deoxynivalenol, zearalenone, and T-2 toxin. These kits provided reliable qualitative, but not quantitative, data.</description><subject>Agronomy. Soil science and plant productions</subject><subject>ALIMENTOS</subject><subject>ANALYTICAL METHODS</subject><subject>BARLEY</subject><subject>Biological and medical sciences</subject><subject>BLE</subject><subject>Canada</subject><subject>CEBADA</subject><subject>CHEMICAL COMPOSITION</subject><subject>Chromatography, High Pressure Liquid</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>CONTAMINACION</subject><subject>CONTAMINATION</subject><subject>DETECTION</subject><subject>Edible Grain - chemistry</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>FOOD SAFETY</subject><subject>Food toxicology</subject><subject>FOODS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>HPLC</subject><subject>INNOCUITE DES PRODUITS ALIMENTAIRES</subject><subject>INOCUIDAD ALIMENTARIA</subject><subject>MICOTOXINAS</subject><subject>MYCOTOXINE</subject><subject>MYCOTOXINS</subject><subject>Mycotoxins - analysis</subject><subject>ORGE</subject><subject>Pathology, epidemiology, host-fungus relationships. Damages, economic importance</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>PRODUIT ALIMENTAIRE</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>TRIGO</subject><subject>WHEATS</subject><issn>0090-4341</issn><issn>1432-0703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EKkvhwhEJyQfEASll_NE4PpYVpZVW4lB6jib-2Bgl8dZOVux_j1e7KkdOHvn99EbvDSHvGVwxAPX12y0wxhsl4QVZMSl4BQrES7IC0FBJIdlr8ibn3wCFauQFuWikAi2aFdlv3N4NmUZPfdg7Oh5MnOOfMGUaJrpNeJx6THuXZ2ePfzfzgNMcDF3jhBYpZjo6zEsqcnegfdj2dOeSj2nEyTg6hKclWGr6FEecY7Hc9Ye35JXHIbt35_eSPN5-_7W-qzY_f9yvbzaVEaDmitdSWMPUdVd3nQYh0DDfaXbNlS9htDbO8waVQM6tbOraWGNs6QKcrC3U4pJ8PvnuUnxaSoZ2DNm4oURwccktqxstGOf_ByXXSrKmgF9OoEkx5-R8u0thxHRoGbTHa7T_rlHgj2fXpRudfUbP9Rf901nHbHDwqTQW8jMmVdnKVME-nDCPscVtKsjjg645SCbEXwO4meA</recordid><startdate>199304</startdate><enddate>199304</enddate><creator>Stratton, G.W. (Department of Biology, Nova Scotia, Canada.)</creator><creator>Robinson, A.R</creator><creator>Smith, H.C</creator><creator>Kittilsen, L</creator><creator>Barbour, M</creator><general>Springer-Verlag</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>199304</creationdate><title>Levels of five mycotoxins in grains harvested in Atlantic Canada as measured by high performance liquid chromatography</title><author>Stratton, G.W. (Department of Biology, Nova Scotia, Canada.) ; Robinson, A.R ; Smith, H.C ; Kittilsen, L ; Barbour, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c307t-2643dc175b6bb9033ac1fb91527f00199cef28a73a22d4866cdccd1120e46d063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>ALIMENTOS</topic><topic>ANALYTICAL METHODS</topic><topic>BARLEY</topic><topic>Biological and medical sciences</topic><topic>BLE</topic><topic>Canada</topic><topic>CEBADA</topic><topic>CHEMICAL COMPOSITION</topic><topic>Chromatography, High Pressure Liquid</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>CONTAMINACION</topic><topic>CONTAMINATION</topic><topic>DETECTION</topic><topic>Edible Grain - chemistry</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>FOOD SAFETY</topic><topic>Food toxicology</topic><topic>FOODS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>HPLC</topic><topic>INNOCUITE DES PRODUITS ALIMENTAIRES</topic><topic>INOCUIDAD ALIMENTARIA</topic><topic>MICOTOXINAS</topic><topic>MYCOTOXINE</topic><topic>MYCOTOXINS</topic><topic>Mycotoxins - analysis</topic><topic>ORGE</topic><topic>Pathology, epidemiology, host-fungus relationships. Damages, economic importance</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>PRODUIT ALIMENTAIRE</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>TRIGO</topic><topic>WHEATS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stratton, G.W. (Department of Biology, Nova Scotia, Canada.)</creatorcontrib><creatorcontrib>Robinson, A.R</creatorcontrib><creatorcontrib>Smith, H.C</creatorcontrib><creatorcontrib>Kittilsen, L</creatorcontrib><creatorcontrib>Barbour, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Archives of environmental contamination and toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stratton, G.W. (Department of Biology, Nova Scotia, Canada.)</au><au>Robinson, A.R</au><au>Smith, H.C</au><au>Kittilsen, L</au><au>Barbour, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Levels of five mycotoxins in grains harvested in Atlantic Canada as measured by high performance liquid chromatography</atitle><jtitle>Archives of environmental contamination and toxicology</jtitle><addtitle>Arch Environ Contam Toxicol</addtitle><date>1993-04</date><risdate>1993</risdate><volume>24</volume><issue>3</issue><spage>399</spage><epage>409</epage><pages>399-409</pages><issn>0090-4341</issn><eissn>1432-0703</eissn><coden>AECTCV</coden><abstract>An analytical protocol using high performance liquid chromatography (HPLC) was used to analyze samples of spring wheat, winter wheat, 2-row barley, and 6-row barley over a period of three years for the presence of five mycotoxins. These included deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, and diacetoxyscirpenol. The protocol employed a single extraction step using acetonitrile-water and two cleanup procedures. One utilized a solid-phase extraction column and the other a charcoal-alumina column. Detection limits ranged from 0.02 to 0.15 micrograms of mycotoxin g-1 grain. Little T-2 toxin, HT-2 toxin, or diacetoxyscirpenol was found in the samples. Deoxynivalenol was detected in 53 to 62% of the samples tested and zearalenone in 25-29% of the samples. Several enzyme-linked immunosorbent assays were used for comparison purposes with deoxynivalenol, zearalenone, and T-2 toxin. These kits provided reliable qualitative, but not quantitative, data.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><cop>New York, NY</cop><pub>Springer-Verlag</pub><pmid>8470938</pmid><doi>10.1007/BF01128740</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0090-4341 |
| ispartof | Archives of environmental contamination and toxicology, 1993-04, Vol.24 (3), p.399-409 |
| issn | 0090-4341 1432-0703 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16893122 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Agronomy. Soil science and plant productions ALIMENTOS ANALYTICAL METHODS BARLEY Biological and medical sciences BLE Canada CEBADA CHEMICAL COMPOSITION Chromatography, High Pressure Liquid COMPOSICION QUIMICA COMPOSITION CHIMIQUE CONTAMINACION CONTAMINATION DETECTION Edible Grain - chemistry Food Contamination - analysis Food industries FOOD SAFETY Food toxicology FOODS Fundamental and applied biological sciences. Psychology Fungal plant pathogens HPLC INNOCUITE DES PRODUITS ALIMENTAIRES INOCUIDAD ALIMENTARIA MICOTOXINAS MYCOTOXINE MYCOTOXINS Mycotoxins - analysis ORGE Pathology, epidemiology, host-fungus relationships. Damages, economic importance Phytopathology. Animal pests. Plant and forest protection PRODUIT ALIMENTAIRE TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS TRIGO WHEATS |
| title | Levels of five mycotoxins in grains harvested in Atlantic Canada as measured by high performance liquid chromatography |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T14%3A05%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Levels%20of%20five%20mycotoxins%20in%20grains%20harvested%20in%20Atlantic%20Canada%20as%20measured%20by%20high%20performance%20liquid%20chromatography&rft.jtitle=Archives%20of%20environmental%20contamination%20and%20toxicology&rft.au=Stratton,%20G.W.%20(Department%20of%20Biology,%20Nova%20Scotia,%20Canada.)&rft.date=1993-04&rft.volume=24&rft.issue=3&rft.spage=399&rft.epage=409&rft.pages=399-409&rft.issn=0090-4341&rft.eissn=1432-0703&rft.coden=AECTCV&rft_id=info:doi/10.1007/BF01128740&rft_dat=%3Cproquest_cross%3E16893122%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14297418&rft_id=info:pmid/8470938&rfr_iscdi=true |