Expression of Homeobox-containing Genes in Common Carp Cyprinus Carpio L

Expression of Homeobox-containing Genes in Common Carp Cyprinus Carpio L

分子遺傳學家在研究果蠅體節突變機制的過程，發現了控制體節發生位置的基因，稱之為同位基因（homeotic gene）。所有同位基因皆包含由60個保留性氨基酸組成之同位胜肽區（homeodomain），其180個鹼基核甘酸對序列稱為同位序列（homeo box）。利用果蠅觸角足（Antennapedia）基因（Antp）同位序列當探針，南氏轉移雜合法（Southern hybridization）證明鯉魚至少有兩種Antp型同位基因存在，RNA slot雜合法證明鯉魚Antp型同位基因主要表現於腸、眼、腎、心等器官，在肝、睪丸、小腦亦有少量表現，而在大腦、腦垂體、肌肉則不表現。利用北方轉移雜合法...

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Veröffentlicht in:Zoological Studies 1995-07, Vol.34 (3), p.170-176
Hauptverfasser: 成之綱(Jr-Gang Cheng), 廖欽峰(Ching-Fong Liao), 徐亞莉(Ya-Li Hsu), 吳金洌(Jen-Leih Wu)
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Cyprinus carpio
Freshwater
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container_end_page 176
container_issue 3
container_start_page 170
container_title Zoological Studies
container_volume 34
creator 成之綱(Jr-Gang Cheng)
廖欽峰(Ching-Fong Liao)
徐亞莉(Ya-Li Hsu)
吳金洌(Jen-Leih Wu)
description 分子遺傳學家在研究果蠅體節突變機制的過程，發現了控制體節發生位置的基因，稱之為同位基因（homeotic gene）。所有同位基因皆包含由60個保留性氨基酸組成之同位胜肽區（homeodomain），其180個鹼基核甘酸對序列稱為同位序列（homeo box）。利用果蠅觸角足（Antennapedia）基因（Antp）同位序列當探針，南氏轉移雜合法（Southern hybridization）證明鯉魚至少有兩種Antp型同位基因存在，RNA slot雜合法證明鯉魚Antp型同位基因主要表現於腸、眼、腎、心等器官，在肝、睪丸、小腦亦有少量表現，而在大腦、腦垂體、肌肉則不表現。利用北方轉移雜合法（Northern hybridization）證明，鯉魚胚胎發生時期，至少有五種Antp型同位基因轉錄本（transcript）存在，而其表現之強弱視胚胎發生階段而定。
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Cyprinus carpio
Freshwater
title Expression of Homeobox-containing Genes in Common Carp Cyprinus Carpio L
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