Analysis of lifespan-promoting effect of garlic extract by an integrated metabolo-proteomics approach
The beneficial effects of garlic (Allium sativum) consumption in treating human diseases have been reported worldwide over a long period of human history. The strong antioxidant effect of garlic extract (GE) has also recently been claimed to prevent cancer, thrombus formation, cardiovascular disease...
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| Veröffentlicht in: | The Journal of nutritional biochemistry 2015-08, Vol.26 (8), p.808-817 |
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| creator | Huang, Chun-Hao Hsu, Fang-Yu Wu, Yuan-Heng Zhong, Linda Tseng, Mu-Yun Kuo, Chao-Jen Hsu, Ao-Lin Liang, Shih-Shin Chiou, Shyh-Horng |
| description | The beneficial effects of garlic (Allium sativum) consumption in treating human diseases have been reported worldwide over a long period of human history. The strong antioxidant effect of garlic extract (GE) has also recently been claimed to prevent cancer, thrombus formation, cardiovascular disease and some age-related maladies. Using Caenorhabditis elegans as a model organism, aqueous GE was herein shown to increase the expression of longevity-related FOXO transcription factor daf-16 and extend lifespan by 20%. By employing microarray and proteomics analysis on C. elegans treated with aqueous GE, we have systematically mapped 229 genes and 46 proteins with differential expression profiles, which included many metabolic enzymes and yolky egg vitellogenins. To investigate the garlic components functionally involved in longevity, an integrated metabolo-proteomics approach was employed to identify metabolites and protein components associated with treatment of aqueous GE. Among potential lifespan-promoting substances, mannose-binding lectin and N-acetylcysteine were found to increase daf-16 expression. Our study points to the fact that the lifespan-promoting effect of aqueous GE may entail the DAF-16-mediated signaling pathway. The result also highlights the utility of metabolo-proteomics for unraveling the complexity and intricacy involved in the metabolism of natural products in vivo.
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| doi_str_mv | 10.1016/j.jnutbio.2015.02.010 |
| format | Article |
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[Display omitted]</description><identifier>ISSN: 0955-2863</identifier><identifier>EISSN: 1873-4847</identifier><identifier>DOI: 10.1016/j.jnutbio.2015.02.010</identifier><identifier>PMID: 25940980</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetylcysteine - metabolism ; Aging ; Amino Acid Sequence ; Animals ; C. elegans ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans Proteins - genetics ; Caenorhabditis elegans Proteins - metabolism ; Chromatography, Liquid ; Cloning, Molecular ; DAF-16 ; Down-Regulation ; Evolution, Molecular ; Forkhead Transcription Factors - genetics ; Forkhead Transcription Factors - metabolism ; Garlic - chemistry ; Garlic extract ; Longevity - drug effects ; Mannose-binding lectin ; Mannose-Binding Lectin - metabolism ; Metabolo-proteomics ; Metabolomics - methods ; Molecular Sequence Data ; N-Acetylcysteine ; Plant Extracts - pharmacology ; Proteomics - methods ; Signal Transduction ; Tandem Mass Spectrometry ; Up-Regulation ; Vitellogenins - genetics ; Vitellogenins - metabolism</subject><ispartof>The Journal of nutritional biochemistry, 2015-08, Vol.26 (8), p.808-817</ispartof><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-fb634ab14bbb166356d3c7be7700924cc86dac5c0dc025e9f7c0fe092854b5823</citedby><cites>FETCH-LOGICAL-c365t-fb634ab14bbb166356d3c7be7700924cc86dac5c0dc025e9f7c0fe092854b5823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jnutbio.2015.02.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25940980$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Chun-Hao</creatorcontrib><creatorcontrib>Hsu, Fang-Yu</creatorcontrib><creatorcontrib>Wu, Yuan-Heng</creatorcontrib><creatorcontrib>Zhong, Linda</creatorcontrib><creatorcontrib>Tseng, Mu-Yun</creatorcontrib><creatorcontrib>Kuo, Chao-Jen</creatorcontrib><creatorcontrib>Hsu, Ao-Lin</creatorcontrib><creatorcontrib>Liang, Shih-Shin</creatorcontrib><creatorcontrib>Chiou, Shyh-Horng</creatorcontrib><title>Analysis of lifespan-promoting effect of garlic extract by an integrated metabolo-proteomics approach</title><title>The Journal of nutritional biochemistry</title><addtitle>J Nutr Biochem</addtitle><description>The beneficial effects of garlic (Allium sativum) consumption in treating human diseases have been reported worldwide over a long period of human history. The strong antioxidant effect of garlic extract (GE) has also recently been claimed to prevent cancer, thrombus formation, cardiovascular disease and some age-related maladies. Using Caenorhabditis elegans as a model organism, aqueous GE was herein shown to increase the expression of longevity-related FOXO transcription factor daf-16 and extend lifespan by 20%. By employing microarray and proteomics analysis on C. elegans treated with aqueous GE, we have systematically mapped 229 genes and 46 proteins with differential expression profiles, which included many metabolic enzymes and yolky egg vitellogenins. To investigate the garlic components functionally involved in longevity, an integrated metabolo-proteomics approach was employed to identify metabolites and protein components associated with treatment of aqueous GE. Among potential lifespan-promoting substances, mannose-binding lectin and N-acetylcysteine were found to increase daf-16 expression. Our study points to the fact that the lifespan-promoting effect of aqueous GE may entail the DAF-16-mediated signaling pathway. The result also highlights the utility of metabolo-proteomics for unraveling the complexity and intricacy involved in the metabolism of natural products in vivo.
[Display omitted]</description><subject>Acetylcysteine - metabolism</subject><subject>Aging</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>C. elegans</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans Proteins - genetics</subject><subject>Caenorhabditis elegans Proteins - metabolism</subject><subject>Chromatography, Liquid</subject><subject>Cloning, Molecular</subject><subject>DAF-16</subject><subject>Down-Regulation</subject><subject>Evolution, Molecular</subject><subject>Forkhead Transcription Factors - genetics</subject><subject>Forkhead Transcription Factors - metabolism</subject><subject>Garlic - chemistry</subject><subject>Garlic extract</subject><subject>Longevity - drug effects</subject><subject>Mannose-binding lectin</subject><subject>Mannose-Binding Lectin - metabolism</subject><subject>Metabolo-proteomics</subject><subject>Metabolomics - methods</subject><subject>Molecular Sequence Data</subject><subject>N-Acetylcysteine</subject><subject>Plant Extracts - pharmacology</subject><subject>Proteomics - methods</subject><subject>Signal Transduction</subject><subject>Tandem Mass Spectrometry</subject><subject>Up-Regulation</subject><subject>Vitellogenins - genetics</subject><subject>Vitellogenins - metabolism</subject><issn>0955-2863</issn><issn>1873-4847</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFP3DAQha2qFSyUn9AqRy4JY8d2khNCqC1ISFzo2bKd8darJF5sL2L_fb3apdeeRjPz3jzNR8g3Cg0FKm82zWbZZeNDw4CKBlgDFD6RFe27tuY97z6TFQxC1KyX7Tm5SGkDAIwLeUbOmRg4DD2sCN4tetonn6rgqsk7TFu91NsY5pD9sq7QObT5sFzrOHlb4XuOukzMvtJL5ZeM66gzjtWMWZswhYM5Y5i9TZXelkbbP1_JF6enhFenekl-__zxcv9QPz3_ery_e6ptK0WunZEt14ZyYwyVshVybG1nsOsABsat7eWorbAwWmACB9dZcFhWveBG9Ky9JNfHuyX2dYcpq9kni9OkFwy7pKjshxaGQfAiFUepjSGliE5to5913CsK6kBYbdSJsDoQVsBUIVx8308ROzPj-M_1gbQIbo8CLI--eYwqWY-LxdHHglKNwf8n4i_FFJFe</recordid><startdate>201508</startdate><enddate>201508</enddate><creator>Huang, Chun-Hao</creator><creator>Hsu, Fang-Yu</creator><creator>Wu, Yuan-Heng</creator><creator>Zhong, Linda</creator><creator>Tseng, Mu-Yun</creator><creator>Kuo, Chao-Jen</creator><creator>Hsu, Ao-Lin</creator><creator>Liang, Shih-Shin</creator><creator>Chiou, Shyh-Horng</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201508</creationdate><title>Analysis of lifespan-promoting effect of garlic extract by an integrated metabolo-proteomics approach</title><author>Huang, Chun-Hao ; Hsu, Fang-Yu ; Wu, Yuan-Heng ; Zhong, Linda ; Tseng, Mu-Yun ; Kuo, Chao-Jen ; Hsu, Ao-Lin ; Liang, Shih-Shin ; Chiou, Shyh-Horng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-fb634ab14bbb166356d3c7be7700924cc86dac5c0dc025e9f7c0fe092854b5823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acetylcysteine - metabolism</topic><topic>Aging</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>C. elegans</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans Proteins - genetics</topic><topic>Caenorhabditis elegans Proteins - metabolism</topic><topic>Chromatography, Liquid</topic><topic>Cloning, Molecular</topic><topic>DAF-16</topic><topic>Down-Regulation</topic><topic>Evolution, Molecular</topic><topic>Forkhead Transcription Factors - genetics</topic><topic>Forkhead Transcription Factors - metabolism</topic><topic>Garlic - chemistry</topic><topic>Garlic extract</topic><topic>Longevity - drug effects</topic><topic>Mannose-binding lectin</topic><topic>Mannose-Binding Lectin - metabolism</topic><topic>Metabolo-proteomics</topic><topic>Metabolomics - methods</topic><topic>Molecular Sequence Data</topic><topic>N-Acetylcysteine</topic><topic>Plant Extracts - pharmacology</topic><topic>Proteomics - methods</topic><topic>Signal Transduction</topic><topic>Tandem Mass Spectrometry</topic><topic>Up-Regulation</topic><topic>Vitellogenins - genetics</topic><topic>Vitellogenins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Chun-Hao</creatorcontrib><creatorcontrib>Hsu, Fang-Yu</creatorcontrib><creatorcontrib>Wu, Yuan-Heng</creatorcontrib><creatorcontrib>Zhong, Linda</creatorcontrib><creatorcontrib>Tseng, Mu-Yun</creatorcontrib><creatorcontrib>Kuo, Chao-Jen</creatorcontrib><creatorcontrib>Hsu, Ao-Lin</creatorcontrib><creatorcontrib>Liang, Shih-Shin</creatorcontrib><creatorcontrib>Chiou, Shyh-Horng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutritional biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Chun-Hao</au><au>Hsu, Fang-Yu</au><au>Wu, Yuan-Heng</au><au>Zhong, Linda</au><au>Tseng, Mu-Yun</au><au>Kuo, Chao-Jen</au><au>Hsu, Ao-Lin</au><au>Liang, Shih-Shin</au><au>Chiou, Shyh-Horng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of lifespan-promoting effect of garlic extract by an integrated metabolo-proteomics approach</atitle><jtitle>The Journal of nutritional biochemistry</jtitle><addtitle>J Nutr Biochem</addtitle><date>2015-08</date><risdate>2015</risdate><volume>26</volume><issue>8</issue><spage>808</spage><epage>817</epage><pages>808-817</pages><issn>0955-2863</issn><eissn>1873-4847</eissn><abstract>The beneficial effects of garlic (Allium sativum) consumption in treating human diseases have been reported worldwide over a long period of human history. The strong antioxidant effect of garlic extract (GE) has also recently been claimed to prevent cancer, thrombus formation, cardiovascular disease and some age-related maladies. Using Caenorhabditis elegans as a model organism, aqueous GE was herein shown to increase the expression of longevity-related FOXO transcription factor daf-16 and extend lifespan by 20%. By employing microarray and proteomics analysis on C. elegans treated with aqueous GE, we have systematically mapped 229 genes and 46 proteins with differential expression profiles, which included many metabolic enzymes and yolky egg vitellogenins. To investigate the garlic components functionally involved in longevity, an integrated metabolo-proteomics approach was employed to identify metabolites and protein components associated with treatment of aqueous GE. Among potential lifespan-promoting substances, mannose-binding lectin and N-acetylcysteine were found to increase daf-16 expression. Our study points to the fact that the lifespan-promoting effect of aqueous GE may entail the DAF-16-mediated signaling pathway. The result also highlights the utility of metabolo-proteomics for unraveling the complexity and intricacy involved in the metabolism of natural products in vivo.
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| ispartof | The Journal of nutritional biochemistry, 2015-08, Vol.26 (8), p.808-817 |
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| subjects | Acetylcysteine - metabolism Aging Amino Acid Sequence Animals C. elegans Caenorhabditis elegans - genetics Caenorhabditis elegans Proteins - genetics Caenorhabditis elegans Proteins - metabolism Chromatography, Liquid Cloning, Molecular DAF-16 Down-Regulation Evolution, Molecular Forkhead Transcription Factors - genetics Forkhead Transcription Factors - metabolism Garlic - chemistry Garlic extract Longevity - drug effects Mannose-binding lectin Mannose-Binding Lectin - metabolism Metabolo-proteomics Metabolomics - methods Molecular Sequence Data N-Acetylcysteine Plant Extracts - pharmacology Proteomics - methods Signal Transduction Tandem Mass Spectrometry Up-Regulation Vitellogenins - genetics Vitellogenins - metabolism |
| title | Analysis of lifespan-promoting effect of garlic extract by an integrated metabolo-proteomics approach |
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