Integrin-based meningioma cell migration is promoted by photon but not by carbon-ion irradiation
Purpose Sublethal doses of photon irradiation (IR) are suspected to increase tumor cell migration and support locoregional recurrence of disease, which has already been shown in other cell lines. This manuscript describes the effect of photon and carbon-ion IR on WHO class I meningioma cell migratio...
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| Veröffentlicht in: | Strahlentherapie und Onkologie 2015-04, Vol.191 (4), p.347-355 |
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| creator | Simon, Florian Dittmar, Jan-Oliver Brons, Stephan Orschiedt, Lena Urbschat, Steffi Weber, Klaus-Josef Debus, Jürgen Combs, Stephanie E. Rieken, Stefan |
| description | Purpose
Sublethal doses of photon irradiation (IR) are suspected to increase tumor cell migration and support locoregional recurrence of disease, which has already been shown in other cell lines. This manuscript describes the effect of photon and carbon-ion IR on WHO class I meningioma cell migration and provides an approach to the underlying cellular mechanisms.
Materials and methods
Meningioma cells were gained operatively at the university hospital in Homburg/Saar, Germany. For migration, membranes (8-µm pore sizes) were coated with collagen I, with collagen IV, and with fibronectin. Cells were analyzed in migration experiments with or without serum stimulation, with or without photon and carbon IR 24 h prior to experiments, and with or without integrin antibodies. Fluorescence-activated cell sorting (FACS) analyses of the integrins ανβ
1
, ανβ
3
, and ανβ
5
were performed without IR and 6, 12 and 24 h after IR. Enzyme-linked immunosorbent assay (ELISA) analyses of matrix metalloproteinases (MMP)-2 and MMP-9 were realized with and without IR after cells were cultured on collagen I, collagen IV, or fibronectin for 24 h. Cells and supernatants for FACS and ELISA were stored at − 18 °C. The significance level was set at 5 % using both Student’s
t
test and two-way ANOVA.
Results
Migration of meningioma cells was serum-inducible (
p
|
| doi_str_mv | 10.1007/s00066-014-0778-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1689308139</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1689308139</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-723364a780aafd7d02b31d7f10976c6ea7707644df7697bbea8fc45d66b4872b3</originalsourceid><addsrcrecordid>eNp1kM1qGzEURkVpqB23D9BNGegmGyVXMxrd0TKE_BgM2aTQnSrNaFwZj-RKMwu_feTYKSGQleDqfJ-uDiHfGVwyALxKACAEBcYpIDZ0_4nMGa8kBSl_fyZzYCgpsrqZkfOUNgBMcMm_kFlZc16zup6TP0s_2nV0nhqdbFcM1ju_dmHQRWu322Jw66hHF3zhUrGLYQhjpsy-2P0NY56aaSx8GA-TVkcTPH1hY9Sde8l9JWe93ib77XQuyK-726ebB7p6vF_eXK9oW2E5UiyrSnCNDWjdd9hBaSrWYc9AomiF1YiAgvOuRyHRGKubvuV1J4ThDWZ4QS6OvXnJf5NNoxpcOnxBexumpJhoZAUNq2RGf75DN2GKPm-XKeRMlqypM8WOVBtDStH2ahfdoONeMVAH_eqoX2X96qBf7XPmx6l5MoPt_idefWegPAIpX_m1jW-e_rD1GUFJkLc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1674192185</pqid></control><display><type>article</type><title>Integrin-based meningioma cell migration is promoted by photon but not by carbon-ion irradiation</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Simon, Florian ; Dittmar, Jan-Oliver ; Brons, Stephan ; Orschiedt, Lena ; Urbschat, Steffi ; Weber, Klaus-Josef ; Debus, Jürgen ; Combs, Stephanie E. ; Rieken, Stefan</creator><creatorcontrib>Simon, Florian ; Dittmar, Jan-Oliver ; Brons, Stephan ; Orschiedt, Lena ; Urbschat, Steffi ; Weber, Klaus-Josef ; Debus, Jürgen ; Combs, Stephanie E. ; Rieken, Stefan</creatorcontrib><description>Purpose
Sublethal doses of photon irradiation (IR) are suspected to increase tumor cell migration and support locoregional recurrence of disease, which has already been shown in other cell lines. This manuscript describes the effect of photon and carbon-ion IR on WHO class I meningioma cell migration and provides an approach to the underlying cellular mechanisms.
Materials and methods
Meningioma cells were gained operatively at the university hospital in Homburg/Saar, Germany. For migration, membranes (8-µm pore sizes) were coated with collagen I, with collagen IV, and with fibronectin. Cells were analyzed in migration experiments with or without serum stimulation, with or without photon and carbon IR 24 h prior to experiments, and with or without integrin antibodies. Fluorescence-activated cell sorting (FACS) analyses of the integrins ανβ
1
, ανβ
3
, and ανβ
5
were performed without IR and 6, 12 and 24 h after IR. Enzyme-linked immunosorbent assay (ELISA) analyses of matrix metalloproteinases (MMP)-2 and MMP-9 were realized with and without IR after cells were cultured on collagen I, collagen IV, or fibronectin for 24 h. Cells and supernatants for FACS and ELISA were stored at − 18 °C. The significance level was set at 5 % using both Student’s
t
test and two-way ANOVA.
Results
Migration of meningioma cells was serum-inducible (
p
< 0.001). It could be increased by photon IR (
p
< 0.02). The integrins ανβ
1
and ανβ
5
showed a 21 and 11 % higher expression after serum stimulation (not significant), respectively, and ανβ
1
expression was raised by 14 % (
p
= 0.0057) after photon IR. Antibody blockage of the integrins ανβ
1
and ανβ
5
inhibited serum- and photon-induced migration. Expression of MMP-2 and MMP-9 remained unchanged after both IR and fetal bovine serum (FBS). Carbon-ion IR left both integrin expression and meningioma cell migration unaffected.
Conclusion
Photon but not carbon-ion IR promotes serum-based meningioma cell migration. Fibronectin receptor integrin ανβ
1
signaling can be identified as an important mechanism for serum- and photon-induced migration of WHO class I meningioma cells.</description><identifier>ISSN: 0179-7158</identifier><identifier>EISSN: 1439-099X</identifier><identifier>DOI: 10.1007/s00066-014-0778-y</identifier><identifier>PMID: 25445155</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Carbon ; Cell Movement - immunology ; Cell Movement - radiation effects ; Cells, Cultured ; Dose-Response Relationship, Radiation ; Heavy Ions ; Humans ; Integrin alphaV - immunology ; Medicine ; Medicine & Public Health ; Meningeal Neoplasms - immunology ; Meningeal Neoplasms - pathology ; Meningioma - immunology ; Meningioma - pathology ; Oncology ; Original Article ; Protons ; Radiation Dosage ; Radiotherapy</subject><ispartof>Strahlentherapie und Onkologie, 2015-04, Vol.191 (4), p.347-355</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-723364a780aafd7d02b31d7f10976c6ea7707644df7697bbea8fc45d66b4872b3</citedby><cites>FETCH-LOGICAL-c372t-723364a780aafd7d02b31d7f10976c6ea7707644df7697bbea8fc45d66b4872b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00066-014-0778-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00066-014-0778-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25445155$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simon, Florian</creatorcontrib><creatorcontrib>Dittmar, Jan-Oliver</creatorcontrib><creatorcontrib>Brons, Stephan</creatorcontrib><creatorcontrib>Orschiedt, Lena</creatorcontrib><creatorcontrib>Urbschat, Steffi</creatorcontrib><creatorcontrib>Weber, Klaus-Josef</creatorcontrib><creatorcontrib>Debus, Jürgen</creatorcontrib><creatorcontrib>Combs, Stephanie E.</creatorcontrib><creatorcontrib>Rieken, Stefan</creatorcontrib><title>Integrin-based meningioma cell migration is promoted by photon but not by carbon-ion irradiation</title><title>Strahlentherapie und Onkologie</title><addtitle>Strahlenther Onkol</addtitle><addtitle>Strahlenther Onkol</addtitle><description>Purpose
Sublethal doses of photon irradiation (IR) are suspected to increase tumor cell migration and support locoregional recurrence of disease, which has already been shown in other cell lines. This manuscript describes the effect of photon and carbon-ion IR on WHO class I meningioma cell migration and provides an approach to the underlying cellular mechanisms.
Materials and methods
Meningioma cells were gained operatively at the university hospital in Homburg/Saar, Germany. For migration, membranes (8-µm pore sizes) were coated with collagen I, with collagen IV, and with fibronectin. Cells were analyzed in migration experiments with or without serum stimulation, with or without photon and carbon IR 24 h prior to experiments, and with or without integrin antibodies. Fluorescence-activated cell sorting (FACS) analyses of the integrins ανβ
1
, ανβ
3
, and ανβ
5
were performed without IR and 6, 12 and 24 h after IR. Enzyme-linked immunosorbent assay (ELISA) analyses of matrix metalloproteinases (MMP)-2 and MMP-9 were realized with and without IR after cells were cultured on collagen I, collagen IV, or fibronectin for 24 h. Cells and supernatants for FACS and ELISA were stored at − 18 °C. The significance level was set at 5 % using both Student’s
t
test and two-way ANOVA.
Results
Migration of meningioma cells was serum-inducible (
p
< 0.001). It could be increased by photon IR (
p
< 0.02). The integrins ανβ
1
and ανβ
5
showed a 21 and 11 % higher expression after serum stimulation (not significant), respectively, and ανβ
1
expression was raised by 14 % (
p
= 0.0057) after photon IR. Antibody blockage of the integrins ανβ
1
and ανβ
5
inhibited serum- and photon-induced migration. Expression of MMP-2 and MMP-9 remained unchanged after both IR and fetal bovine serum (FBS). Carbon-ion IR left both integrin expression and meningioma cell migration unaffected.
Conclusion
Photon but not carbon-ion IR promotes serum-based meningioma cell migration. Fibronectin receptor integrin ανβ
1
signaling can be identified as an important mechanism for serum- and photon-induced migration of WHO class I meningioma cells.</description><subject>Carbon</subject><subject>Cell Movement - immunology</subject><subject>Cell Movement - radiation effects</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Heavy Ions</subject><subject>Humans</subject><subject>Integrin alphaV - immunology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Meningeal Neoplasms - immunology</subject><subject>Meningeal Neoplasms - pathology</subject><subject>Meningioma - immunology</subject><subject>Meningioma - pathology</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Protons</subject><subject>Radiation Dosage</subject><subject>Radiotherapy</subject><issn>0179-7158</issn><issn>1439-099X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kM1qGzEURkVpqB23D9BNGegmGyVXMxrd0TKE_BgM2aTQnSrNaFwZj-RKMwu_feTYKSGQleDqfJ-uDiHfGVwyALxKACAEBcYpIDZ0_4nMGa8kBSl_fyZzYCgpsrqZkfOUNgBMcMm_kFlZc16zup6TP0s_2nV0nhqdbFcM1ju_dmHQRWu322Jw66hHF3zhUrGLYQhjpsy-2P0NY56aaSx8GA-TVkcTPH1hY9Sde8l9JWe93ib77XQuyK-726ebB7p6vF_eXK9oW2E5UiyrSnCNDWjdd9hBaSrWYc9AomiF1YiAgvOuRyHRGKubvuV1J4ThDWZ4QS6OvXnJf5NNoxpcOnxBexumpJhoZAUNq2RGf75DN2GKPm-XKeRMlqypM8WOVBtDStH2ahfdoONeMVAH_eqoX2X96qBf7XPmx6l5MoPt_idefWegPAIpX_m1jW-e_rD1GUFJkLc</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Simon, Florian</creator><creator>Dittmar, Jan-Oliver</creator><creator>Brons, Stephan</creator><creator>Orschiedt, Lena</creator><creator>Urbschat, Steffi</creator><creator>Weber, Klaus-Josef</creator><creator>Debus, Jürgen</creator><creator>Combs, Stephanie E.</creator><creator>Rieken, Stefan</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20150401</creationdate><title>Integrin-based meningioma cell migration is promoted by photon but not by carbon-ion irradiation</title><author>Simon, Florian ; Dittmar, Jan-Oliver ; Brons, Stephan ; Orschiedt, Lena ; Urbschat, Steffi ; Weber, Klaus-Josef ; Debus, Jürgen ; Combs, Stephanie E. ; Rieken, Stefan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-723364a780aafd7d02b31d7f10976c6ea7707644df7697bbea8fc45d66b4872b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Carbon</topic><topic>Cell Movement - immunology</topic><topic>Cell Movement - radiation effects</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Heavy Ions</topic><topic>Humans</topic><topic>Integrin alphaV - immunology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Meningeal Neoplasms - immunology</topic><topic>Meningeal Neoplasms - pathology</topic><topic>Meningioma - immunology</topic><topic>Meningioma - pathology</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Protons</topic><topic>Radiation Dosage</topic><topic>Radiotherapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simon, Florian</creatorcontrib><creatorcontrib>Dittmar, Jan-Oliver</creatorcontrib><creatorcontrib>Brons, Stephan</creatorcontrib><creatorcontrib>Orschiedt, Lena</creatorcontrib><creatorcontrib>Urbschat, Steffi</creatorcontrib><creatorcontrib>Weber, Klaus-Josef</creatorcontrib><creatorcontrib>Debus, Jürgen</creatorcontrib><creatorcontrib>Combs, Stephanie E.</creatorcontrib><creatorcontrib>Rieken, Stefan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Strahlentherapie und Onkologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simon, Florian</au><au>Dittmar, Jan-Oliver</au><au>Brons, Stephan</au><au>Orschiedt, Lena</au><au>Urbschat, Steffi</au><au>Weber, Klaus-Josef</au><au>Debus, Jürgen</au><au>Combs, Stephanie E.</au><au>Rieken, Stefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrin-based meningioma cell migration is promoted by photon but not by carbon-ion irradiation</atitle><jtitle>Strahlentherapie und Onkologie</jtitle><stitle>Strahlenther Onkol</stitle><addtitle>Strahlenther Onkol</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>191</volume><issue>4</issue><spage>347</spage><epage>355</epage><pages>347-355</pages><issn>0179-7158</issn><eissn>1439-099X</eissn><abstract>Purpose
Sublethal doses of photon irradiation (IR) are suspected to increase tumor cell migration and support locoregional recurrence of disease, which has already been shown in other cell lines. This manuscript describes the effect of photon and carbon-ion IR on WHO class I meningioma cell migration and provides an approach to the underlying cellular mechanisms.
Materials and methods
Meningioma cells were gained operatively at the university hospital in Homburg/Saar, Germany. For migration, membranes (8-µm pore sizes) were coated with collagen I, with collagen IV, and with fibronectin. Cells were analyzed in migration experiments with or without serum stimulation, with or without photon and carbon IR 24 h prior to experiments, and with or without integrin antibodies. Fluorescence-activated cell sorting (FACS) analyses of the integrins ανβ
1
, ανβ
3
, and ανβ
5
were performed without IR and 6, 12 and 24 h after IR. Enzyme-linked immunosorbent assay (ELISA) analyses of matrix metalloproteinases (MMP)-2 and MMP-9 were realized with and without IR after cells were cultured on collagen I, collagen IV, or fibronectin for 24 h. Cells and supernatants for FACS and ELISA were stored at − 18 °C. The significance level was set at 5 % using both Student’s
t
test and two-way ANOVA.
Results
Migration of meningioma cells was serum-inducible (
p
< 0.001). It could be increased by photon IR (
p
< 0.02). The integrins ανβ
1
and ανβ
5
showed a 21 and 11 % higher expression after serum stimulation (not significant), respectively, and ανβ
1
expression was raised by 14 % (
p
= 0.0057) after photon IR. Antibody blockage of the integrins ανβ
1
and ανβ
5
inhibited serum- and photon-induced migration. Expression of MMP-2 and MMP-9 remained unchanged after both IR and fetal bovine serum (FBS). Carbon-ion IR left both integrin expression and meningioma cell migration unaffected.
Conclusion
Photon but not carbon-ion IR promotes serum-based meningioma cell migration. Fibronectin receptor integrin ανβ
1
signaling can be identified as an important mechanism for serum- and photon-induced migration of WHO class I meningioma cells.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25445155</pmid><doi>10.1007/s00066-014-0778-y</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| issn | 0179-7158 1439-099X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1689308139 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Carbon Cell Movement - immunology Cell Movement - radiation effects Cells, Cultured Dose-Response Relationship, Radiation Heavy Ions Humans Integrin alphaV - immunology Medicine Medicine & Public Health Meningeal Neoplasms - immunology Meningeal Neoplasms - pathology Meningioma - immunology Meningioma - pathology Oncology Original Article Protons Radiation Dosage Radiotherapy |
| title | Integrin-based meningioma cell migration is promoted by photon but not by carbon-ion irradiation |
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