Detection of Carbofuran and Other Carbamates Using Cholinesterase Inhibition with N-Methylindoxyl Acetate as Substrate

Trace amounts of carbamate insecticide residues in meat extracts were detected based on cholinesterase inhibition. Assay solutions with inhibitory chemicals remain colorless when substrate is added. Both fluorescence and a yellow color mark negatives and negative controls, followed by generation of a blue precipitate that can act as a permanent marker for analyses judged negative Meat samples with carbamate pesticide concentrations that exceed tolerance levels can be rapidly identified.