Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression

Pichia pastoris is a methylotrophic yeast increasingly important in the production of therapeutic proteins. Expression vectors are based on the methanol–inducibte AOX1 promoter and are integrated into the host chromosome. In most cases high copy number integration has been shown to be important for...

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Veröffentlicht in:	Bio/Technology 1994-02, Vol.12 (2), p.181-184
Hauptverfasser:	Scorer, C.A, Clare, J.J, McCombie, W.R, Romanos, M.A, Sreekrishna, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture ANTIBIOTICOS ANTIBIOTICS ANTIBIOTIQUE aox1 promoter ARN MENSAJERO ARN MESSAGER Base Sequence Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Blotting, Northern Blotting, Western CHEMICAL RESISTANCE Cloning, Molecular - methods Drug Resistance, Microbial DRUGS EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE GENE EXPRESSION Gene Products, env - biosynthesis Gene Products, env - isolation & purification GENE TRANSFER GENES Genes, Fungal Genetic engineering Genetic technics GENETIC TRANSFORMATION Genetic Vectors Gentamicins - pharmacology HIV Envelope Protein gp120 - biosynthesis HIV Envelope Protein gp120 - genetics HIV-1 - genetics IN VITRO SELECTION kanr gene Life Sciences MEDICAMENT MEDICAMENTOS MESSENGER RNA Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Mutagenesis, Site-Directed PICHIA Pichia - drug effects Pichia - genetics Pichia - metabolism Pichia pastoris PLASMID VECTORS Promoter Regions, Genetic PROMOTERS Recombinant Proteins - biosynthesis REPORTER GENES RESISTANCE AUX PRODUITS CHIMIQUES RESISTENCIA QUIMICA Restriction Mapping RNA, Messenger - biosynthesis SELECCION IN VITRO SELECTION IN VITRO TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transformation, Genetic
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