Differential expression of Dickkopf-1 among non-small cell lung cancer cells
Dickkopf-1 (DKK1) is a negative regulator of the Wnt/β-catenin signaling pathway, which is expressed in various human cancers. It was hypothesized that DKK1 was oncogenic and involved in invasive growth in non-small cell lung cancer (NSCLC) cells. The present study aimed to investigate whether DKK1...
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| Veröffentlicht in: | Molecular medicine reports 2015-08, Vol.12 (2), p.1935-1940 |
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| description | Dickkopf-1 (DKK1) is a negative regulator of the Wnt/β-catenin signaling pathway, which is expressed in various human cancers. It was hypothesized that DKK1 was oncogenic and involved in invasive growth in non-small cell lung cancer (NSCLC) cells. The present study aimed to investigate whether DKK1 gene expression levels differ among various NSCLC cells. The DKK1 expression pattern was analyzed in various human NSCLC cell lines and tissues. The DKK1 protein and gene expression levels were quantified using immunoblotting, polymerase chain reaction analysis and immunohistochemistry. The majority of the lung cancer cell lines analyzed revealed increased expression levels of DKK1. Furthermore, DKK1 expression was highly transactivated in the majority of these cancer cell lines. Clinical samples were obtained from 98 NSCLC patients for immunohistochemical analysis. Of the 98 samples analyzed, 62 (63.3%) demonstrated positive staining for DKK1, whereas the remaining 36 (37%) exhibited negative staining. However, no immunohistopathological staining was detected in normal tissues. The relative effects of DKK1 were assessed in a high-expression cell line (LTEP-a-2) and a low-expression cell line (95D). The differential expression of genes involved in cell cycle, apoptosis, signaling pathway, invasion and metastasis were evaluated, relative to DKK1 levels. In conclusion, the results of the present study indicated that DKK1 functioned as a key regulator in the progression of NSCLC. The results confirmed the differential expression of DKK1 in NSCLC cells, which may present a potential therapeutic target for cancer prevention. |
| doi_str_mv | 10.3892/mmr.2015.3654 |
| format | Article |
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It was hypothesized that DKK1 was oncogenic and involved in invasive growth in non-small cell lung cancer (NSCLC) cells. The present study aimed to investigate whether DKK1 gene expression levels differ among various NSCLC cells. The DKK1 expression pattern was analyzed in various human NSCLC cell lines and tissues. The DKK1 protein and gene expression levels were quantified using immunoblotting, polymerase chain reaction analysis and immunohistochemistry. The majority of the lung cancer cell lines analyzed revealed increased expression levels of DKK1. Furthermore, DKK1 expression was highly transactivated in the majority of these cancer cell lines. Clinical samples were obtained from 98 NSCLC patients for immunohistochemical analysis. Of the 98 samples analyzed, 62 (63.3%) demonstrated positive staining for DKK1, whereas the remaining 36 (37%) exhibited negative staining. However, no immunohistopathological staining was detected in normal tissues. The relative effects of DKK1 were assessed in a high-expression cell line (LTEP-a-2) and a low-expression cell line (95D). The differential expression of genes involved in cell cycle, apoptosis, signaling pathway, invasion and metastasis were evaluated, relative to DKK1 levels. In conclusion, the results of the present study indicated that DKK1 functioned as a key regulator in the progression of NSCLC. The results confirmed the differential expression of DKK1 in NSCLC cells, which may present a potential therapeutic target for cancer prevention.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2015.3654</identifier><identifier>PMID: 25901391</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Analysis ; Apoptosis ; cancer prevention ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - pathology ; Cell cycle ; Cell Line, Tumor ; Development and progression ; Dickkopf-1 ; differential expression ; Dkk1 protein ; Esophagus ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Humans ; Immunoblotting ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins - analysis ; Intercellular Signaling Peptides and Proteins - genetics ; Invasiveness ; Laboratories ; Lung - pathology ; Lung cancer ; Lung cancer, Non-small cell ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; Medical prognosis ; Metastases ; Metastasis ; Multiple myeloma ; Mutation ; non-small cell lung cancer ; Non-small cell lung carcinoma ; Patients ; Polymerase chain reaction ; Proteins ; Rodents ; Signal transduction ; Studies ; Tumor cell lines ; Tumors ; Wnt protein ; β-catenin</subject><ispartof>Molecular medicine reports, 2015-08, Vol.12 (2), p.1935-1940</ispartof><rights>Copyright © 2015, Spandidos Publications</rights><rights>COPYRIGHT 2015 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c595t-ca9dfa5e9bca300c0e182dcce69fdee2b69ba9b9c90b34c1e5b05e1e8e4c4eb03</citedby><cites>FETCH-LOGICAL-c595t-ca9dfa5e9bca300c0e182dcce69fdee2b69ba9b9c90b34c1e5b05e1e8e4c4eb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,5569,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25901391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>XIANG, XIAO JUN</creatorcontrib><creatorcontrib>LIU, YA WEN</creatorcontrib><creatorcontrib>CHEN, DIAN DIAN</creatorcontrib><creatorcontrib>YU, SHUANG</creatorcontrib><title>Differential expression of Dickkopf-1 among non-small cell lung cancer cells</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Dickkopf-1 (DKK1) is a negative regulator of the Wnt/β-catenin signaling pathway, which is expressed in various human cancers. It was hypothesized that DKK1 was oncogenic and involved in invasive growth in non-small cell lung cancer (NSCLC) cells. The present study aimed to investigate whether DKK1 gene expression levels differ among various NSCLC cells. The DKK1 expression pattern was analyzed in various human NSCLC cell lines and tissues. The DKK1 protein and gene expression levels were quantified using immunoblotting, polymerase chain reaction analysis and immunohistochemistry. The majority of the lung cancer cell lines analyzed revealed increased expression levels of DKK1. Furthermore, DKK1 expression was highly transactivated in the majority of these cancer cell lines. Clinical samples were obtained from 98 NSCLC patients for immunohistochemical analysis. Of the 98 samples analyzed, 62 (63.3%) demonstrated positive staining for DKK1, whereas the remaining 36 (37%) exhibited negative staining. However, no immunohistopathological staining was detected in normal tissues. The relative effects of DKK1 were assessed in a high-expression cell line (LTEP-a-2) and a low-expression cell line (95D). The differential expression of genes involved in cell cycle, apoptosis, signaling pathway, invasion and metastasis were evaluated, relative to DKK1 levels. In conclusion, the results of the present study indicated that DKK1 functioned as a key regulator in the progression of NSCLC. The results confirmed the differential expression of DKK1 in NSCLC cells, which may present a potential therapeutic target for cancer prevention.</description><subject>Analysis</subject><subject>Apoptosis</subject><subject>cancer prevention</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - pathology</subject><subject>Cell cycle</subject><subject>Cell Line, Tumor</subject><subject>Development and progression</subject><subject>Dickkopf-1</subject><subject>differential expression</subject><subject>Dkk1 protein</subject><subject>Esophagus</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Intercellular Signaling Peptides and Proteins - analysis</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Invasiveness</subject><subject>Laboratories</subject><subject>Lung - pathology</subject><subject>Lung cancer</subject><subject>Lung cancer, Non-small cell</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - pathology</subject><subject>Medical prognosis</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Multiple myeloma</subject><subject>Mutation</subject><subject>non-small cell lung cancer</subject><subject>Non-small cell lung carcinoma</subject><subject>Patients</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Signal transduction</subject><subject>Studies</subject><subject>Tumor cell lines</subject><subject>Tumors</subject><subject>Wnt protein</subject><subject>β-catenin</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkc1r3DAQxUVIaNK0x1yDIYf2oq0kS_LqGDb9CCzk0pyFLI8WbWzJkdaQ_PeRs5vQliIYDcNvHm94CF1QsqiXin0bhrRghIpFLQU_Qme0URTXhPDjQ8-Uak7Rx5y3hEjBhPqATksltFb0DK1vvHOQIOy86St4GhPk7GOooqtuvH14iKPDtDJDDJsqxIDzYPq-slBKP5WZNcFCeh3kT-jEmT7D58N_ju5_fP-9-oXXdz9vV9drbIUSO2yN6pwRoFprilNLgC5ZZy1I5ToA1krVGtUqq0hbc0tBtEQAhSVwy6El9Tn6utcdU3ycIO_04PPswASIU9ZULptytZC8oFf_oNs4pVDcaapqxhvWiD-ojelB--DiLhk7i-przoRsuJSiUIv_UOV1MHgbAzhf5n8t4P2CTTHnBE6PyQ8mPWtK9JyeLunpOT09p1f4y4PZqR2ge6ff4irAlz2QRxM638X8zhQlTBkmDJe7RP0CwdGhWQ</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>XIANG, XIAO JUN</creator><creator>LIU, YA WEN</creator><creator>CHEN, DIAN DIAN</creator><creator>YU, SHUANG</creator><general>D.A. 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LIU, YA WEN ; CHEN, DIAN DIAN ; YU, SHUANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c595t-ca9dfa5e9bca300c0e182dcce69fdee2b69ba9b9c90b34c1e5b05e1e8e4c4eb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Apoptosis</topic><topic>cancer prevention</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - pathology</topic><topic>Cell cycle</topic><topic>Cell Line, Tumor</topic><topic>Development and progression</topic><topic>Dickkopf-1</topic><topic>differential expression</topic><topic>Dkk1 protein</topic><topic>Esophagus</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Intercellular Signaling Peptides and Proteins - analysis</topic><topic>Intercellular Signaling Peptides and Proteins - genetics</topic><topic>Invasiveness</topic><topic>Laboratories</topic><topic>Lung - pathology</topic><topic>Lung cancer</topic><topic>Lung cancer, Non-small cell</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - pathology</topic><topic>Medical prognosis</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Multiple myeloma</topic><topic>Mutation</topic><topic>non-small cell lung cancer</topic><topic>Non-small cell lung carcinoma</topic><topic>Patients</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Rodents</topic><topic>Signal transduction</topic><topic>Studies</topic><topic>Tumor cell lines</topic><topic>Tumors</topic><topic>Wnt protein</topic><topic>β-catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>XIANG, XIAO JUN</creatorcontrib><creatorcontrib>LIU, YA WEN</creatorcontrib><creatorcontrib>CHEN, DIAN DIAN</creatorcontrib><creatorcontrib>YU, SHUANG</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>XIANG, XIAO JUN</au><au>LIU, YA WEN</au><au>CHEN, DIAN DIAN</au><au>YU, SHUANG</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of Dickkopf-1 among non-small cell lung cancer cells</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>12</volume><issue>2</issue><spage>1935</spage><epage>1940</epage><pages>1935-1940</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>Dickkopf-1 (DKK1) is a negative regulator of the Wnt/β-catenin signaling pathway, which is expressed in various human cancers. It was hypothesized that DKK1 was oncogenic and involved in invasive growth in non-small cell lung cancer (NSCLC) cells. The present study aimed to investigate whether DKK1 gene expression levels differ among various NSCLC cells. The DKK1 expression pattern was analyzed in various human NSCLC cell lines and tissues. The DKK1 protein and gene expression levels were quantified using immunoblotting, polymerase chain reaction analysis and immunohistochemistry. The majority of the lung cancer cell lines analyzed revealed increased expression levels of DKK1. Furthermore, DKK1 expression was highly transactivated in the majority of these cancer cell lines. Clinical samples were obtained from 98 NSCLC patients for immunohistochemical analysis. Of the 98 samples analyzed, 62 (63.3%) demonstrated positive staining for DKK1, whereas the remaining 36 (37%) exhibited negative staining. However, no immunohistopathological staining was detected in normal tissues. The relative effects of DKK1 were assessed in a high-expression cell line (LTEP-a-2) and a low-expression cell line (95D). The differential expression of genes involved in cell cycle, apoptosis, signaling pathway, invasion and metastasis were evaluated, relative to DKK1 levels. In conclusion, the results of the present study indicated that DKK1 functioned as a key regulator in the progression of NSCLC. The results confirmed the differential expression of DKK1 in NSCLC cells, which may present a potential therapeutic target for cancer prevention.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>25901391</pmid><doi>10.3892/mmr.2015.3654</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Apoptosis cancer prevention Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - pathology Cell cycle Cell Line, Tumor Development and progression Dickkopf-1 differential expression Dkk1 protein Esophagus Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Humans Immunoblotting Immunohistochemistry Intercellular Signaling Peptides and Proteins - analysis Intercellular Signaling Peptides and Proteins - genetics Invasiveness Laboratories Lung - pathology Lung cancer Lung cancer, Non-small cell Lung Neoplasms - genetics Lung Neoplasms - pathology Medical prognosis Metastases Metastasis Multiple myeloma Mutation non-small cell lung cancer Non-small cell lung carcinoma Patients Polymerase chain reaction Proteins Rodents Signal transduction Studies Tumor cell lines Tumors Wnt protein β-catenin |
| title | Differential expression of Dickkopf-1 among non-small cell lung cancer cells |
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