Analysis of actinobacteria community and diversity in the pit mud of Chinese luzhou-flavour liquor

In this study, the pit mud of Luzhou-flavour liquor which was used for 1, 50, 100 and 400 years were taken from a factory affiliated to the Luzhou Laojiao Group. Eight mixed samples were sampled from the wall and bottom layers of the pit mud in the cellars. Denaturing gradient gel electrophoresis (DGGE) was employed to analyze actinobacteria community structure and diversity from the samples. For the analysis, 16S rRNA fragments about 400 bp amplified from DNA extracted from the eight mixed pit mud samples. This result revealed the abundant diversity of actinobacteria in the pit mud of Chinese Luzhou-flavour liquor. The actinobacteria community structure and diversity were different among different samples, whereas the community succession is regular. To our knowledge, this was the first time the community and diversity of acinobacteria in pit mud were reported.