Pokemon proto-oncogene in oral cancer: potential role in the early phase of tumorigenesis
Objectives Oral squamous cell carcinoma (OSCC) represents about 90% of all oral neoplasms with a poor clinical prognosis. To improve survival of OSCC patients, it is fundamental to understand the basic molecular mechanisms characterizing oral carcinogenesis. Dysregulation of oncogenes and tumor supp...
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| Veröffentlicht in: | Oral diseases 2015-05, Vol.21 (4), p.462-469 |
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| creator | Sartini, D Lo Muzio, L Morganti, S Pozzi, V Di Ruscio, G Rocchetti, R Rubini, C Santarelli, A Emanuelli, M |
| description | Objectives
Oral squamous cell carcinoma (OSCC) represents about 90% of all oral neoplasms with a poor clinical prognosis. To improve survival of OSCC patients, it is fundamental to understand the basic molecular mechanisms characterizing oral carcinogenesis. Dysregulation of oncogenes and tumor suppressor genes seems to play a central role in tumorigenesis, including malignant transformation of the oral cavity.
Materials and Methods
We analyzed the expression levels of the pro‐oncogenic transcription factor Pokemon through real‐time PCR, Western blot and immunohistochemistry in tumor, and normal oral tissue samples obtained from 22 patients with OSCC. The relationship between tumor characteristics and the level of Pokemon intratumor expression was also analyzed.
Results
Pokemon was significantly downregulated in OSCC. In particular, both mRNA and protein levels (tumor vs normal tissue) inversely correlated with histological grading, suggesting its potential role as a prognostic factor for OSCC. Moreover, a significant inverse correlation was found between Pokemon protein expression levels (OSCC vs normal oral mucosa) and tumor size, supporting the hypothesis that Pokemon could play an important role in the early phase of tumor expansion.
Conclusion
This work shows that reduced expression of Pokemon is a peculiar feature of OSCC. Additional studies may establish the effective role of Pokemon in oral tumorigenesis. |
| doi_str_mv | 10.1111/odi.12304 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1687669630</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1687669630</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4944-234e627dbfaaccae4645ef457a5bbb8ca2aedac712c5a1f5b189a7a6d48988243</originalsourceid><addsrcrecordid>eNp1kE9PFjEQxhujEUQPfgGziRc9LPR_u94MKJIQ4ICiXprZ7qwUdrev7W70_fYWXuBA4lxm0vk9T6YPIa8Z3WWl9mIXdhkXVD4h20xTVlPL1dMyCyVrxcX3LfIi5ytKmWkEf062uJKioUpskx9n8RrHOFWrFOdYx8nHXzhhFaYqJhgqD5PH9KFaxRmnOZSXFIfb9XyJFUIa1tXqEjJWsa_mZYwp3OhzyC_Jsx6GjK_u-g75-vnT-f6X-vj08Gj_43HtZSNlzYVEzU3X9gDeA0otFfZSGVBt21oPHLADbxj3ClivWmYbMKA7aRtruRQ75N3Gt_zg94J5dmPIHocBJoxLdkxbo3WjBS3o20foVVzSVK4rlOFWC2VFod5vKJ9izgl7t0phhLR2jLqbvF3J293mXdg3d45LO2L3QN4HXIC9DfAnDLj-v5M7PTi6t6w3ipBn_PuggHTttBFGuYuTQ2e-_Ty40Gfnjot_xWaZQA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1672863583</pqid></control><display><type>article</type><title>Pokemon proto-oncogene in oral cancer: potential role in the early phase of tumorigenesis</title><source>MEDLINE</source><source>Wiley Online Library Journals</source><creator>Sartini, D ; Lo Muzio, L ; Morganti, S ; Pozzi, V ; Di Ruscio, G ; Rocchetti, R ; Rubini, C ; Santarelli, A ; Emanuelli, M</creator><creatorcontrib>Sartini, D ; Lo Muzio, L ; Morganti, S ; Pozzi, V ; Di Ruscio, G ; Rocchetti, R ; Rubini, C ; Santarelli, A ; Emanuelli, M</creatorcontrib><description>Objectives
Oral squamous cell carcinoma (OSCC) represents about 90% of all oral neoplasms with a poor clinical prognosis. To improve survival of OSCC patients, it is fundamental to understand the basic molecular mechanisms characterizing oral carcinogenesis. Dysregulation of oncogenes and tumor suppressor genes seems to play a central role in tumorigenesis, including malignant transformation of the oral cavity.
Materials and Methods
We analyzed the expression levels of the pro‐oncogenic transcription factor Pokemon through real‐time PCR, Western blot and immunohistochemistry in tumor, and normal oral tissue samples obtained from 22 patients with OSCC. The relationship between tumor characteristics and the level of Pokemon intratumor expression was also analyzed.
Results
Pokemon was significantly downregulated in OSCC. In particular, both mRNA and protein levels (tumor vs normal tissue) inversely correlated with histological grading, suggesting its potential role as a prognostic factor for OSCC. Moreover, a significant inverse correlation was found between Pokemon protein expression levels (OSCC vs normal oral mucosa) and tumor size, supporting the hypothesis that Pokemon could play an important role in the early phase of tumor expansion.
Conclusion
This work shows that reduced expression of Pokemon is a peculiar feature of OSCC. Additional studies may establish the effective role of Pokemon in oral tumorigenesis.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.12304</identifier><identifier>PMID: 25439053</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Aged ; Aged, 80 and over ; Biomarkers, Tumor - biosynthesis ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Carcinogenesis - genetics ; Carcinogenesis - metabolism ; Carcinogenesis - pathology ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; DNA-Binding Proteins - biosynthesis ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Down-Regulation ; Female ; Genes ; Head and Neck Neoplasms - genetics ; Head and Neck Neoplasms - metabolism ; Head and Neck Neoplasms - pathology ; histological grading ; Humans ; Male ; Medical prognosis ; Middle Aged ; Mouth Mucosa - pathology ; Mouth Neoplasms - genetics ; Mouth Neoplasms - metabolism ; Mouth Neoplasms - pathology ; Oral cancer ; Pokemon ; Proto-Oncogenes ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Rodents ; Squamous Cell Carcinoma of Head and Neck ; Transcription Factors - biosynthesis ; Transcription Factors - genetics ; Transcription Factors - metabolism ; tumor size ; Tumorigenesis</subject><ispartof>Oral diseases, 2015-05, Vol.21 (4), p.462-469</ispartof><rights>2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4944-234e627dbfaaccae4645ef457a5bbb8ca2aedac712c5a1f5b189a7a6d48988243</citedby><cites>FETCH-LOGICAL-c4944-234e627dbfaaccae4645ef457a5bbb8ca2aedac712c5a1f5b189a7a6d48988243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.12304$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.12304$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25439053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sartini, D</creatorcontrib><creatorcontrib>Lo Muzio, L</creatorcontrib><creatorcontrib>Morganti, S</creatorcontrib><creatorcontrib>Pozzi, V</creatorcontrib><creatorcontrib>Di Ruscio, G</creatorcontrib><creatorcontrib>Rocchetti, R</creatorcontrib><creatorcontrib>Rubini, C</creatorcontrib><creatorcontrib>Santarelli, A</creatorcontrib><creatorcontrib>Emanuelli, M</creatorcontrib><title>Pokemon proto-oncogene in oral cancer: potential role in the early phase of tumorigenesis</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objectives
Oral squamous cell carcinoma (OSCC) represents about 90% of all oral neoplasms with a poor clinical prognosis. To improve survival of OSCC patients, it is fundamental to understand the basic molecular mechanisms characterizing oral carcinogenesis. Dysregulation of oncogenes and tumor suppressor genes seems to play a central role in tumorigenesis, including malignant transformation of the oral cavity.
Materials and Methods
We analyzed the expression levels of the pro‐oncogenic transcription factor Pokemon through real‐time PCR, Western blot and immunohistochemistry in tumor, and normal oral tissue samples obtained from 22 patients with OSCC. The relationship between tumor characteristics and the level of Pokemon intratumor expression was also analyzed.
Results
Pokemon was significantly downregulated in OSCC. In particular, both mRNA and protein levels (tumor vs normal tissue) inversely correlated with histological grading, suggesting its potential role as a prognostic factor for OSCC. Moreover, a significant inverse correlation was found between Pokemon protein expression levels (OSCC vs normal oral mucosa) and tumor size, supporting the hypothesis that Pokemon could play an important role in the early phase of tumor expansion.
Conclusion
This work shows that reduced expression of Pokemon is a peculiar feature of OSCC. Additional studies may establish the effective role of Pokemon in oral tumorigenesis.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers, Tumor - biosynthesis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Carcinogenesis - genetics</subject><subject>Carcinogenesis - metabolism</subject><subject>Carcinogenesis - pathology</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>DNA-Binding Proteins - biosynthesis</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Genes</subject><subject>Head and Neck Neoplasms - genetics</subject><subject>Head and Neck Neoplasms - metabolism</subject><subject>Head and Neck Neoplasms - pathology</subject><subject>histological grading</subject><subject>Humans</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Middle Aged</subject><subject>Mouth Mucosa - pathology</subject><subject>Mouth Neoplasms - genetics</subject><subject>Mouth Neoplasms - metabolism</subject><subject>Mouth Neoplasms - pathology</subject><subject>Oral cancer</subject><subject>Pokemon</subject><subject>Proto-Oncogenes</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Rodents</subject><subject>Squamous Cell Carcinoma of Head and Neck</subject><subject>Transcription Factors - biosynthesis</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>tumor size</subject><subject>Tumorigenesis</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE9PFjEQxhujEUQPfgGziRc9LPR_u94MKJIQ4ICiXprZ7qwUdrev7W70_fYWXuBA4lxm0vk9T6YPIa8Z3WWl9mIXdhkXVD4h20xTVlPL1dMyCyVrxcX3LfIi5ytKmWkEf062uJKioUpskx9n8RrHOFWrFOdYx8nHXzhhFaYqJhgqD5PH9KFaxRmnOZSXFIfb9XyJFUIa1tXqEjJWsa_mZYwp3OhzyC_Jsx6GjK_u-g75-vnT-f6X-vj08Gj_43HtZSNlzYVEzU3X9gDeA0otFfZSGVBt21oPHLADbxj3ClivWmYbMKA7aRtruRQ75N3Gt_zg94J5dmPIHocBJoxLdkxbo3WjBS3o20foVVzSVK4rlOFWC2VFod5vKJ9izgl7t0phhLR2jLqbvF3J293mXdg3d45LO2L3QN4HXIC9DfAnDLj-v5M7PTi6t6w3ipBn_PuggHTttBFGuYuTQ2e-_Ty40Gfnjot_xWaZQA</recordid><startdate>201505</startdate><enddate>201505</enddate><creator>Sartini, D</creator><creator>Lo Muzio, L</creator><creator>Morganti, S</creator><creator>Pozzi, V</creator><creator>Di Ruscio, G</creator><creator>Rocchetti, R</creator><creator>Rubini, C</creator><creator>Santarelli, A</creator><creator>Emanuelli, M</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7TO</scope><scope>H94</scope></search><sort><creationdate>201505</creationdate><title>Pokemon proto-oncogene in oral cancer: potential role in the early phase of tumorigenesis</title><author>Sartini, D ; Lo Muzio, L ; Morganti, S ; Pozzi, V ; Di Ruscio, G ; Rocchetti, R ; Rubini, C ; Santarelli, A ; Emanuelli, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4944-234e627dbfaaccae4645ef457a5bbb8ca2aedac712c5a1f5b189a7a6d48988243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers, Tumor - biosynthesis</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Carcinogenesis - genetics</topic><topic>Carcinogenesis - metabolism</topic><topic>Carcinogenesis - pathology</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>DNA-Binding Proteins - biosynthesis</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Genes</topic><topic>Head and Neck Neoplasms - genetics</topic><topic>Head and Neck Neoplasms - metabolism</topic><topic>Head and Neck Neoplasms - pathology</topic><topic>histological grading</topic><topic>Humans</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>Middle Aged</topic><topic>Mouth Mucosa - pathology</topic><topic>Mouth Neoplasms - genetics</topic><topic>Mouth Neoplasms - metabolism</topic><topic>Mouth Neoplasms - pathology</topic><topic>Oral cancer</topic><topic>Pokemon</topic><topic>Proto-Oncogenes</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Rodents</topic><topic>Squamous Cell Carcinoma of Head and Neck</topic><topic>Transcription Factors - biosynthesis</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>tumor size</topic><topic>Tumorigenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sartini, D</creatorcontrib><creatorcontrib>Lo Muzio, L</creatorcontrib><creatorcontrib>Morganti, S</creatorcontrib><creatorcontrib>Pozzi, V</creatorcontrib><creatorcontrib>Di Ruscio, G</creatorcontrib><creatorcontrib>Rocchetti, R</creatorcontrib><creatorcontrib>Rubini, C</creatorcontrib><creatorcontrib>Santarelli, A</creatorcontrib><creatorcontrib>Emanuelli, M</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sartini, D</au><au>Lo Muzio, L</au><au>Morganti, S</au><au>Pozzi, V</au><au>Di Ruscio, G</au><au>Rocchetti, R</au><au>Rubini, C</au><au>Santarelli, A</au><au>Emanuelli, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pokemon proto-oncogene in oral cancer: potential role in the early phase of tumorigenesis</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2015-05</date><risdate>2015</risdate><volume>21</volume><issue>4</issue><spage>462</spage><epage>469</epage><pages>462-469</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Objectives
Oral squamous cell carcinoma (OSCC) represents about 90% of all oral neoplasms with a poor clinical prognosis. To improve survival of OSCC patients, it is fundamental to understand the basic molecular mechanisms characterizing oral carcinogenesis. Dysregulation of oncogenes and tumor suppressor genes seems to play a central role in tumorigenesis, including malignant transformation of the oral cavity.
Materials and Methods
We analyzed the expression levels of the pro‐oncogenic transcription factor Pokemon through real‐time PCR, Western blot and immunohistochemistry in tumor, and normal oral tissue samples obtained from 22 patients with OSCC. The relationship between tumor characteristics and the level of Pokemon intratumor expression was also analyzed.
Results
Pokemon was significantly downregulated in OSCC. In particular, both mRNA and protein levels (tumor vs normal tissue) inversely correlated with histological grading, suggesting its potential role as a prognostic factor for OSCC. Moreover, a significant inverse correlation was found between Pokemon protein expression levels (OSCC vs normal oral mucosa) and tumor size, supporting the hypothesis that Pokemon could play an important role in the early phase of tumor expansion.
Conclusion
This work shows that reduced expression of Pokemon is a peculiar feature of OSCC. Additional studies may establish the effective role of Pokemon in oral tumorigenesis.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>25439053</pmid><doi>10.1111/odi.12304</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Oral diseases, 2015-05, Vol.21 (4), p.462-469 |
| issn | 1354-523X 1601-0825 |
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| subjects | Aged Aged, 80 and over Biomarkers, Tumor - biosynthesis Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Carcinogenesis - genetics Carcinogenesis - metabolism Carcinogenesis - pathology Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Down-Regulation Female Genes Head and Neck Neoplasms - genetics Head and Neck Neoplasms - metabolism Head and Neck Neoplasms - pathology histological grading Humans Male Medical prognosis Middle Aged Mouth Mucosa - pathology Mouth Neoplasms - genetics Mouth Neoplasms - metabolism Mouth Neoplasms - pathology Oral cancer Pokemon Proto-Oncogenes RNA, Messenger - biosynthesis RNA, Messenger - genetics Rodents Squamous Cell Carcinoma of Head and Neck Transcription Factors - biosynthesis Transcription Factors - genetics Transcription Factors - metabolism tumor size Tumorigenesis |
| title | Pokemon proto-oncogene in oral cancer: potential role in the early phase of tumorigenesis |
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