DNA strand displacement reaction for programmable release of biomolecules
Sample cleanup is a major processing step in many analytical assays. Here, we propose an approach to capture-and-release of analytes based on the DNA strand displacement reaction (SDR) and demonstrate its application to a fluoroimmunoassay on beads for a thyroid cancer biomarker, thyroglobulin. The...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2015-05, Vol.51 (39), p.8307-8310 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Ramezani, Hamid Jed Harrison, D |
| description | Sample cleanup is a major processing step in many analytical assays. Here, we propose an approach to capture-and-release of analytes based on the DNA strand displacement reaction (SDR) and demonstrate its application to a fluoroimmunoassay on beads for a thyroid cancer biomarker, thyroglobulin. The SDR-based cleanup showed no interference from matrix molecules in serum. |
| doi_str_mv | 10.1039/c5cc01880c |
| format | Article |
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| ispartof | Chemical communications (Cambridge, England), 2015-05, Vol.51 (39), p.8307-8310 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1687665019 |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Antibodies - chemistry Antibodies - immunology Beads Biomarkers Cleaning Deoxyribonucleic acid Displacement DNA - chemistry Fluoresceins - chemistry Fluoroimmunoassay Humans Mathematical analysis Serum Albumin, Bovine - chemistry Serums Strands Thyroglobulin - blood Thyroglobulin - immunology |
| title | DNA strand displacement reaction for programmable release of biomolecules |
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