A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine
In this study, the histological, immunohistochemical, morphometric, and biochemical changes to pancreatic beta-cells in STZ-induced diabetes were evaluated in rats treated with different doses of caffeine. Fifty adult male Wistar albino rats were divided into five groups: the nondiabetic control gro...
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| Veröffentlicht in: | Folia histochemica et cytobiologica 2014-01, Vol.52 (1), p.42-50 |
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| creator | Abunasef, Siham K Amin, Hanan A Abdel-Hamid, Ghada A |
| description | In this study, the histological, immunohistochemical, morphometric, and biochemical changes to pancreatic beta-cells in STZ-induced diabetes were evaluated in rats treated with different doses of caffeine. Fifty adult male Wistar albino rats were divided into five groups: the nondiabetic control group, the diabetic untreated group, and three diabetic groups treated with different doses of caffeine (10, 50, and 100 mg/kg/day). Blood glucose and serum insulin levels were measured. The pancreata were collected and processed into paraffin sections. They were stained using hematoxylin and eosin (H&E) and Masson trichrome stains. The insulin expression in beta-cells was assessed using immunohistochemistry. Morphometrically, the percentage area of anti-insulin antibody reaction, the percentage of beta-cells per total islet cell number, and the average area of the islets were determined. STZ-induced degenerative changes in beta-cells led to decreases in the number of functioning beta-cells and insulin immunoreactivity and to increases in the number of collagen fibers in the islets. In STZ-treated rats, caffeine significantly decreased blood glucose concentration while increasing blood insulin levels at the highest applied dose. It also induced a significant increase in the number of immunoreactive beta-cells. In conclusion, caffeine may have a protective role in the biochemical and microscopic changes in pancreatic beta-cells in diabetes induced in rats through STZ administration. |
| doi_str_mv | 10.5603/FHC.2014.0005 |
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Fifty adult male Wistar albino rats were divided into five groups: the nondiabetic control group, the diabetic untreated group, and three diabetic groups treated with different doses of caffeine (10, 50, and 100 mg/kg/day). Blood glucose and serum insulin levels were measured. The pancreata were collected and processed into paraffin sections. They were stained using hematoxylin and eosin (H&E) and Masson trichrome stains. The insulin expression in beta-cells was assessed using immunohistochemistry. Morphometrically, the percentage area of anti-insulin antibody reaction, the percentage of beta-cells per total islet cell number, and the average area of the islets were determined. STZ-induced degenerative changes in beta-cells led to decreases in the number of functioning beta-cells and insulin immunoreactivity and to increases in the number of collagen fibers in the islets. In STZ-treated rats, caffeine significantly decreased blood glucose concentration while increasing blood insulin levels at the highest applied dose. It also induced a significant increase in the number of immunoreactive beta-cells. In conclusion, caffeine may have a protective role in the biochemical and microscopic changes in pancreatic beta-cells in diabetes induced in rats through STZ administration.</description><identifier>ISSN: 0239-8508</identifier><identifier>EISSN: 1897-5631</identifier><identifier>DOI: 10.5603/FHC.2014.0005</identifier><identifier>PMID: 24802960</identifier><language>eng</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Animals ; Antibodies ; Beta cells ; Blood ; Blood glucose ; Blood Glucose - drug effects ; Blood Glucose - metabolism ; Blood levels ; Caffeine ; Caffeine - pharmacology ; Cell Count ; Cell number ; Cell Shape - drug effects ; Collagen ; Diabetes ; Diabetes mellitus ; Diabetes Mellitus, Experimental - drug therapy ; Diabetes Mellitus, Experimental - metabolism ; Diabetes Mellitus, Experimental - pathology ; Dose-Response Relationship, Drug ; Glucose ; Immunohistochemistry ; Insulin ; Insulin - blood ; Insulin-Secreting Cells - drug effects ; Insulin-Secreting Cells - metabolism ; Insulin-Secreting Cells - pathology ; Male ; Pancreas ; Paraffin ; Paraffins ; Rats ; Rats, Wistar ; Streptozocin</subject><ispartof>Folia histochemica et cytobiologica, 2014-01, Vol.52 (1), p.42-50</ispartof><rights>Copyright Folia Histochemica et Cytobiologica 2014</rights><rights>2014. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-2126092661a8746d7ea867554cac4ed8678ad454fe5c4be297414ab8f8ebae723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24802960$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abunasef, Siham K</creatorcontrib><creatorcontrib>Amin, Hanan A</creatorcontrib><creatorcontrib>Abdel-Hamid, Ghada A</creatorcontrib><title>A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine</title><title>Folia histochemica et cytobiologica</title><addtitle>Folia Histochem Cytobiol</addtitle><description>In this study, the histological, immunohistochemical, morphometric, and biochemical changes to pancreatic beta-cells in STZ-induced diabetes were evaluated in rats treated with different doses of caffeine. Fifty adult male Wistar albino rats were divided into five groups: the nondiabetic control group, the diabetic untreated group, and three diabetic groups treated with different doses of caffeine (10, 50, and 100 mg/kg/day). Blood glucose and serum insulin levels were measured. The pancreata were collected and processed into paraffin sections. They were stained using hematoxylin and eosin (H&E) and Masson trichrome stains. The insulin expression in beta-cells was assessed using immunohistochemistry. Morphometrically, the percentage area of anti-insulin antibody reaction, the percentage of beta-cells per total islet cell number, and the average area of the islets were determined. STZ-induced degenerative changes in beta-cells led to decreases in the number of functioning beta-cells and insulin immunoreactivity and to increases in the number of collagen fibers in the islets. In STZ-treated rats, caffeine significantly decreased blood glucose concentration while increasing blood insulin levels at the highest applied dose. It also induced a significant increase in the number of immunoreactive beta-cells. In conclusion, caffeine may have a protective role in the biochemical and microscopic changes in pancreatic beta-cells in diabetes induced in rats through STZ administration.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Beta cells</subject><subject>Blood</subject><subject>Blood glucose</subject><subject>Blood Glucose - drug effects</subject><subject>Blood Glucose - metabolism</subject><subject>Blood levels</subject><subject>Caffeine</subject><subject>Caffeine - pharmacology</subject><subject>Cell Count</subject><subject>Cell number</subject><subject>Cell Shape - drug effects</subject><subject>Collagen</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - drug therapy</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Glucose</subject><subject>Immunohistochemistry</subject><subject>Insulin</subject><subject>Insulin - blood</subject><subject>Insulin-Secreting Cells - drug effects</subject><subject>Insulin-Secreting Cells - metabolism</subject><subject>Insulin-Secreting Cells - pathology</subject><subject>Male</subject><subject>Pancreas</subject><subject>Paraffin</subject><subject>Paraffins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Streptozocin</subject><issn>0239-8508</issn><issn>1897-5631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUtLxDAURoMoOj6WbiXgxk3HJM2rSxkcFQQ3ug5peutE2mZsUkR_vRlfCxe6SvLdw8cNB6FjSuZCkvJ8eb2YM0L5nBAittCM6koVQpZ0G80IK6tCC6L30H6MTxmQhNNdtMe4JqySZIbWF3jlYwpdePTOdtgODfZ9Pw3hI3Yr6D_ymKbmFYcW15AsdtB1EfshxyOsU3gLGc3Pxts89w6PNkWcZzZBg198WmFn2xb8AIdop7VdhKOv8wA9LC_vF9fF7d3VzeLitnClZqlglElSMSmp1YrLRoHVUgnBnXUcmnzXtuGCtyAcr4FVilNua91qqC0oVh6gs8_e9RieJ4jJ9D5u9rYDhCkaKrWSkhMl_kcFY1LTUqiMnv5Cn8I0DvkjhsmK6VzHxF8UFSUnNDMkU8Un5cYQ4witWY--t-OrocRs3Jrs1mzcmo3bzJ98tU51D80P_S2zfAdgeZ4H</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Abunasef, Siham K</creator><creator>Amin, Hanan A</creator><creator>Abdel-Hamid, Ghada A</creator><general>Wydawnictwo Via Medica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TO</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>AEUYN</scope><scope>7X8</scope></search><sort><creationdate>20140101</creationdate><title>A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine</title><author>Abunasef, Siham K ; Amin, Hanan A ; Abdel-Hamid, Ghada A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-2126092661a8746d7ea867554cac4ed8678ad454fe5c4be297414ab8f8ebae723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Beta cells</topic><topic>Blood</topic><topic>Blood glucose</topic><topic>Blood Glucose - drug effects</topic><topic>Blood Glucose - metabolism</topic><topic>Blood levels</topic><topic>Caffeine</topic><topic>Caffeine - pharmacology</topic><topic>Cell Count</topic><topic>Cell number</topic><topic>Cell Shape - drug effects</topic><topic>Collagen</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes Mellitus, Experimental - drug therapy</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetes Mellitus, Experimental - pathology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Glucose</topic><topic>Immunohistochemistry</topic><topic>Insulin</topic><topic>Insulin - blood</topic><topic>Insulin-Secreting Cells - drug effects</topic><topic>Insulin-Secreting Cells - metabolism</topic><topic>Insulin-Secreting Cells - pathology</topic><topic>Male</topic><topic>Pancreas</topic><topic>Paraffin</topic><topic>Paraffins</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Streptozocin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abunasef, Siham K</creatorcontrib><creatorcontrib>Amin, Hanan A</creatorcontrib><creatorcontrib>Abdel-Hamid, Ghada A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>East Europe, Central Europe Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>ProQuest One Sustainability</collection><collection>MEDLINE - Academic</collection><jtitle>Folia histochemica et cytobiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abunasef, Siham K</au><au>Amin, Hanan A</au><au>Abdel-Hamid, Ghada A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine</atitle><jtitle>Folia histochemica et cytobiologica</jtitle><addtitle>Folia Histochem Cytobiol</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>52</volume><issue>1</issue><spage>42</spage><epage>50</epage><pages>42-50</pages><issn>0239-8508</issn><eissn>1897-5631</eissn><abstract>In this study, the histological, immunohistochemical, morphometric, and biochemical changes to pancreatic beta-cells in STZ-induced diabetes were evaluated in rats treated with different doses of caffeine. Fifty adult male Wistar albino rats were divided into five groups: the nondiabetic control group, the diabetic untreated group, and three diabetic groups treated with different doses of caffeine (10, 50, and 100 mg/kg/day). Blood glucose and serum insulin levels were measured. The pancreata were collected and processed into paraffin sections. They were stained using hematoxylin and eosin (H&E) and Masson trichrome stains. The insulin expression in beta-cells was assessed using immunohistochemistry. Morphometrically, the percentage area of anti-insulin antibody reaction, the percentage of beta-cells per total islet cell number, and the average area of the islets were determined. STZ-induced degenerative changes in beta-cells led to decreases in the number of functioning beta-cells and insulin immunoreactivity and to increases in the number of collagen fibers in the islets. In STZ-treated rats, caffeine significantly decreased blood glucose concentration while increasing blood insulin levels at the highest applied dose. It also induced a significant increase in the number of immunoreactive beta-cells. In conclusion, caffeine may have a protective role in the biochemical and microscopic changes in pancreatic beta-cells in diabetes induced in rats through STZ administration.</abstract><cop>Poland</cop><pub>Wydawnictwo Via Medica</pub><pmid>24802960</pmid><doi>10.5603/FHC.2014.0005</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies Beta cells Blood Blood glucose Blood Glucose - drug effects Blood Glucose - metabolism Blood levels Caffeine Caffeine - pharmacology Cell Count Cell number Cell Shape - drug effects Collagen Diabetes Diabetes mellitus Diabetes Mellitus, Experimental - drug therapy Diabetes Mellitus, Experimental - metabolism Diabetes Mellitus, Experimental - pathology Dose-Response Relationship, Drug Glucose Immunohistochemistry Insulin Insulin - blood Insulin-Secreting Cells - drug effects Insulin-Secreting Cells - metabolism Insulin-Secreting Cells - pathology Male Pancreas Paraffin Paraffins Rats Rats, Wistar Streptozocin |
| title | A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine |
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